##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062093_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	145968
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.75242518908254	32.0	32.0	32.0	32.0	32.0
2	30.770833333333332	32.0	32.0	32.0	32.0	32.0
3	30.754446179984654	32.0	32.0	32.0	32.0	32.0
4	30.66083662172531	32.0	32.0	32.0	32.0	32.0
5	30.902444371369068	32.0	32.0	32.0	32.0	32.0
6	34.302956812452045	36.0	36.0	36.0	32.0	36.0
7	34.19460429683218	36.0	36.0	36.0	32.0	36.0
8	34.07088539953963	36.0	36.0	36.0	32.0	36.0
9	34.0150512441083	36.0	36.0	36.0	32.0	36.0
10	34.10270744272717	36.0	36.0	36.0	32.0	36.0
11	34.21366326866163	36.0	36.0	36.0	32.0	36.0
12	34.09205442288721	36.0	36.0	36.0	32.0	36.0
13	33.9888194672805	36.0	36.0	36.0	32.0	36.0
14	33.97150745368848	36.0	36.0	36.0	32.0	36.0
15	33.92046886988929	36.0	36.0	36.0	32.0	36.0
16	34.00141126822317	36.0	36.0	36.0	32.0	36.0
17	33.89724460155651	36.0	36.0	36.0	32.0	36.0
18	33.870320892250355	36.0	36.0	36.0	32.0	36.0
19	33.919269976981255	36.0	36.0	36.0	32.0	36.0
20	33.863004220103036	36.0	36.0	36.0	32.0	36.0
21	33.756254795571635	36.0	36.0	36.0	32.0	36.0
22	33.6915693850707	36.0	36.0	36.0	27.0	36.0
23	33.60707141291242	36.0	36.0	36.0	27.0	36.0
24	33.641256987832946	36.0	36.0	36.0	27.0	36.0
25	32.86188068617779	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	3.0
5	13.0
6	21.0
7	8.0
8	33.0
9	20.0
10	7.0
11	3.0
12	6.0
13	7.0
14	91.0
15	112.0
16	186.0
17	229.0
18	277.0
19	379.0
20	541.0
21	610.0
22	805.0
23	911.0
24	1107.0
25	1382.0
26	1683.0
27	2248.0
28	2820.0
29	3675.0
30	4833.0
31	6695.0
32	9604.0
33	13792.0
34	30753.0
35	63114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.741021881808095	16.708000493265555	12.816683337215515	23.734294287710835
2	11.290145785377618	23.001616792721695	37.49177902005919	28.2164584018415
3	19.872163761920422	29.686643647922832	27.15184149950674	23.28935109065001
4	12.227140057826439	19.363635117913862	32.50955780588405	35.89966701837565
5	11.131364352771877	39.43740149386692	34.68717878434866	14.74405536901254
6	32.344351974992115	31.43534831429864	20.096795864924523	16.123503845784718
7	25.516782574105317	32.00279823875561	23.49491790461298	18.985501282526094
8	29.14931996351191	32.500462959787654	21.94497980123593	16.405237275464504
9	27.619367716357203	16.523194479693252	18.296304933842745	37.5611328701068
10	15.622020236666096	30.050420168067227	32.80672268907563	21.52083690619105
11	31.85205005763214	24.42299248037763	19.592870080685	24.13208738130523
12	23.142412205780456	26.793444332393477	30.056871582731343	20.00727187909472
13	32.239852373210404	20.944207775102385	23.29443724146447	23.521502610222743
14	24.25838666090523	21.70072086531273	24.858536183872097	29.182356289909944
15	29.45207828825059	23.59829593397773	26.959778828145904	19.98984694962578
16	22.83196159122085	25.24142661179698	31.31687242798354	20.60973936899863
17	24.464626232971614	25.365947347481928	30.199744831465296	19.96968158808116
18	27.129512783935038	21.58592121145616	32.17861845714913	19.105947547459674
19	22.381305027565208	32.1107271181327	27.794920321456978	17.713047532845113
20	22.41406501779384	23.83620068981123	33.80280724370358	19.946927048691347
21	21.68463205244247	28.931128116514444	25.76935736032255	23.614882470720534
22	21.051476538603975	29.36000658372996	23.553979727598172	26.034537150067894
23	18.628472698351754	29.942681622466612	25.22625675342128	26.202588925760363
24	19.821024480559558	34.04374957141877	24.81451004594391	21.32071590207776
25	24.421482786755984	24.78144905277448	22.64564920773139	28.151418952738144
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	266.0
1	266.0
2	259.0
3	252.0
4	252.0
5	252.0
6	731.5
7	1211.0
8	1211.0
9	1211.0
10	986.5
11	762.0
12	762.0
13	762.0
14	692.0
15	622.0
16	622.0
17	622.0
18	904.5
19	1187.0
20	1187.0
21	1187.0
22	1842.5
23	2498.0
24	2498.0
25	2498.0
26	3590.5
27	4683.0
28	4683.0
29	4683.0
30	5870.5
31	7058.0
32	7058.0
33	7058.0
34	7395.5
35	7733.0
36	7733.0
37	7733.0
38	8535.5
39	9338.0
40	9338.0
41	9338.0
42	12179.0
43	15020.0
44	15020.0
45	15020.0
46	29498.5
47	43977.0
48	43977.0
49	43977.0
50	33556.0
51	23135.0
52	23135.0
53	23135.0
54	16662.0
55	10189.0
56	10189.0
57	10189.0
58	8485.0
59	6781.0
60	6781.0
61	6781.0
62	5892.0
63	5003.0
64	5003.0
65	5003.0
66	4066.0
67	3129.0
68	3129.0
69	3129.0
70	2367.5
71	1606.0
72	1606.0
73	1606.0
74	1192.5
75	779.0
76	779.0
77	779.0
78	604.0
79	429.0
80	429.0
81	429.0
82	292.0
83	155.0
84	155.0
85	155.0
86	111.5
87	68.0
88	68.0
89	68.0
90	47.0
91	26.0
92	26.0
93	26.0
94	16.0
95	6.0
96	6.0
97	6.0
98	30.5
99	55.0
100	55.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013701633234681574
2	0.0
3	0.0
4	0.009591143264277102
5	0.02603310314589499
6	0.0643976762030034
7	0.11098322920092074
8	0.11440863750959114
9	0.12262961745040009
10	0.13222076071467717
11	0.147977638934561
12	0.13770141400854982
13	0.13359092403814535
14	0.11714896415652747
15	0.13633125068508165
16	0.11509371917132522
17	0.12468486243560233
18	0.10961306587745259
19	0.09043077934889839
20	0.08974569768716431
21	0.09043077934889839
22	0.10550257590704812
23	0.07946947276115314
24	0.09454126931930287
25	0.08289488106982353
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	145968.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.057820892250355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.71597993005929	37.72059629507837
2	10.50478941766763	9.4664584018415
3	2.3779838832294358	3.214403156856297
4	0.9289949825148244	1.6743395812780881
5	0.5032689676144139	1.1338101501699003
6	0.3314581116010339	0.896086813548175
7	0.2463129086209518	0.7768826044064452
8	0.15052455526835942	0.5425846760933903
9	0.16876995590694846	0.6843965800723446
>10	0.8681769803861943	7.269401512660309
>50	0.0881861030865136	2.7718404033760824
>100	0.0760225026607876	8.084648690123863
>500	0.02280675079823628	7.980516277540282
>1k	0.01672495058537327	17.78403485695495
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4199	2.8766578976213966	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4064	2.784171873287296	No Hit
TCCATGTACTCTGCGTTGATACCAC	3850	2.637564397676203	No Hit
CCCATGTACTCTGCGTTGATACCAC	3737	2.5601501699002522	No Hit
CATGTACTCTGCGTTGATACCACTG	1961	1.3434451386605282	No Hit
GTATCAACGCAGAGTACATGGGAAG	1704	1.1673791515948702	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1629	1.1159980269648142	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1289	0.8830702619752275	No Hit
TATCAACGCAGAGTACATGGGAAGC	1222	0.8371697906390441	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1214	0.8316891373451716	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1090	0.7467390112901459	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	942	0.6453469253535021	No Hit
GCGTTGATACCACTGCTTCCCATGT	940	0.643976762030034	No Hit
GCTTCCCATGTACTCTGCGTTGATA	932	0.6384961087361614	No Hit
GCGTTGATACCACTGCTTCCATGTA	930	0.6371259454126932	No Hit
CAGTGGTATCAACGCAGAGTACATG	885	0.6062972706346597	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	879	0.6021867806642551	No Hit
CATGGAAGCAGTGGTATCAACGCAG	873	0.5980762906938507	No Hit
GGTATCAACGCAGAGTACATGGGAA	797	0.5460100844020608	No Hit
GTATCAACGCAGAGTACATGGAAGC	753	0.5158664912857612	No Hit
GTATCAACGCAGAGTACTTTTTTTT	739	0.5062753480214841	No Hit
TATCAACGCAGAGTACATGGAAGCA	672	0.46037487668530086	No Hit
TATCAACGCAGAGTACTTTTTTTTT	583	0.3994026087909679	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	579	0.39666228214403154	No Hit
AAAAAGTACTCTGCGTTGATACCAC	574	0.3932368738353612	No Hit
GTACTCTGCGTTGATACCACTGCTT	571	0.39118162885015895	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	440	0.3014359311629946	No Hit
GGTATCAACGCAGAGTACATGGAAG	426	0.29184478789871754	No Hit
GTGGTATCAACGCAGAGTACATGGG	416	0.2849939712813767	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	406	0.278143154664036	No Hit
ATACCACTGCTTCCATGTACTCTGC	404	0.2767729913405678	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	386	0.26444152142935434	No Hit
ATACCACTGCTTCCCATGTACTCTG	365	0.2500548065329387	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	355	0.24320398991559794	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	351	0.24046366326866161	No Hit
GCAGTGGTATCAACGCAGAGTACAT	336	0.23018743834265043	No Hit
AAAGTACTCTGCGTTGATACCACTG	296	0.2027841718732873	No Hit
GTGGTATCAACGCAGAGTACATGGA	294	0.2014140085498191	No Hit
GGTATCAACGCAGAGTACTTTTTTT	285	0.19524827359421243	No Hit
CCATGTACTCTGCGTTGATACCACT	283	0.19387811027074428	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	280	0.19182286528554204	No Hit
CCACTGCTTCCATGTACTCTGCGTT	274	0.18771237531513754	No Hit
CCACTGCTTCCCATGTACTCTGCGT	273	0.18702729365340348	No Hit
TACCACTGCTTCCCATGTACTCTGC	259	0.17743615038912638	No Hit
CATGGGAAGCAGTGGTATCAACGCA	258	0.1767510687273923	No Hit
GTTGATACCACTGCTTCCATGTACT	249	0.17058533377178559	No Hit
GATACCACTGCTTCCCATGTACTCT	244	0.1671599254631152	No Hit
GATACCACTGCTTCCATGTACTCTG	242	0.16578976213964705	No Hit
ATCAACGCAGAGTACATGGGAAGCA	241	0.16510468047791296	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	241	0.16510468047791296	No Hit
ACATGGAAGCAGTGGTATCAACGCA	239	0.16373451715444481	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	236	0.16167927216924258	No Hit
CCCCATGTACTCTGCGTTGATACCA	235	0.1609941905075085	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	231	0.15825386386057216	No Hit
GTTGATACCACTGCTTCCCATGTAC	217	0.1486627205962951	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	215	0.14729255727282692	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	198	0.13564616902334758	No Hit
ACATGGGAAGCAGTGGTATCAACGC	192	0.1315356790529431	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	171	0.11714896415652747	No Hit
CTTCCCATGTACTCTGCGTTGATAC	167	0.11440863750959114	No Hit
CTGTAGGACGTGGAATATGGCAAGA	155	0.1061876575687822	No Hit
CTTCCATGTACTCTGCGTTGATACC	149	0.10207716759837772	No Hit
ATCAACGCAGAGTACATGGAAGCAG	147	0.10070700427490956	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	146	0.1000219226131755	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	146	0.1000219226131755	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	6.850816617340787E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGAGTT	35	9.8503486E-5	19.002745	11
ACTGTTC	30	7.6872285E-4	18.99623	8
TGTATAT	30	7.6872285E-4	18.99623	2
TGAGTTA	40	2.7366335E-4	16.627401	12
CAATGAG	40	2.743809E-4	16.6217	9
ATACCAA	35	0.0021505237	16.293653	19
TTACAAT	35	0.0021554613	16.288067	16
GTTACAA	35	0.0021554613	16.288067	15
TATATCA	35	0.0021604088	16.282482	4
TCAATGA	35	0.0021604088	16.282482	8
GTATATC	35	0.0021604088	16.282482	3
CTGTTCT	35	0.0021604088	16.282482	9
CACTGTT	45	6.720612E-4	14.774845	7
AATGAGT	45	6.720612E-4	14.774845	10
ATATCAA	40	0.0052557327	14.247172	5
TGTTCTA	55	1.9443309E-4	13.815438	10
GAGTTAC	50	0.001486594	13.30192	13
TATACAC	50	0.0014904445	13.297361	3
GATATAC	50	0.0014904445	13.297361	1
TCCTACA	70	1.07989144E-4	12.211862	2
>>END_MODULE