##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062093_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	145968
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.92420256494574	32.0	32.0	32.0	32.0	32.0
2	31.39439466184369	32.0	32.0	32.0	32.0	32.0
3	31.49950674120355	32.0	32.0	32.0	32.0	32.0
4	31.599638276882605	32.0	32.0	32.0	32.0	32.0
5	31.588690671928095	32.0	32.0	32.0	32.0	32.0
6	35.15408171654061	36.0	36.0	36.0	36.0	36.0
7	35.15150580949249	36.0	36.0	36.0	36.0	36.0
8	35.15590403376083	36.0	36.0	36.0	36.0	36.0
9	35.152163487887755	36.0	36.0	36.0	36.0	36.0
10	35.083929354379045	36.0	36.0	36.0	36.0	36.0
11	35.16261783404582	36.0	36.0	36.0	36.0	36.0
12	35.07470130439548	36.0	36.0	36.0	36.0	36.0
13	35.06684341773539	36.0	36.0	36.0	36.0	36.0
14	35.03179463992108	36.0	36.0	36.0	36.0	36.0
15	35.008796448536664	36.0	36.0	36.0	36.0	36.0
16	35.00291159706237	36.0	36.0	36.0	36.0	36.0
17	34.98420201688041	36.0	36.0	36.0	36.0	36.0
18	34.942898443494464	36.0	36.0	36.0	36.0	36.0
19	34.92897758412803	36.0	36.0	36.0	36.0	36.0
20	34.89857365998027	36.0	36.0	36.0	32.0	36.0
21	34.77445056450729	36.0	36.0	36.0	32.0	36.0
22	34.6970226350981	36.0	36.0	36.0	32.0	36.0
23	34.55528609010194	36.0	36.0	36.0	32.0	36.0
24	34.561999890386936	36.0	36.0	36.0	32.0	36.0
25	34.518695878548726	36.0	36.0	36.0	32.0	36.0
26	34.39805984873397	36.0	36.0	36.0	32.0	36.0
27	34.35120711388797	36.0	36.0	36.0	32.0	36.0
28	34.287706894661845	36.0	36.0	36.0	32.0	36.0
29	34.15656171215609	36.0	36.0	36.0	32.0	36.0
30	34.186691603639154	36.0	36.0	36.0	32.0	36.0
31	34.00323358544338	36.0	36.0	36.0	32.0	36.0
32	34.0634865175929	36.0	36.0	36.0	32.0	36.0
33	33.921722569330264	36.0	36.0	36.0	32.0	36.0
34	33.82185821549929	36.0	36.0	36.0	32.0	36.0
35	33.67537405458731	36.0	36.0	36.0	27.0	36.0
36	33.5021717088677	36.0	36.0	36.0	21.0	36.0
37	33.37025238408418	36.0	36.0	36.0	21.0	36.0
38	33.19989312726077	36.0	36.0	36.0	21.0	36.0
39	32.81463745478461	36.0	36.0	36.0	14.0	36.0
40	32.543879480434065	36.0	36.0	36.0	14.0	36.0
41	32.49999314918338	36.0	36.0	36.0	14.0	36.0
42	32.263749588951	36.0	36.0	36.0	14.0	36.0
43	32.06874794475502	36.0	32.0	36.0	14.0	36.0
44	32.3048750411049	36.0	36.0	36.0	14.0	36.0
45	31.6681806971391	36.0	32.0	36.0	14.0	36.0
46	31.908473089992327	36.0	32.0	36.0	14.0	36.0
47	31.739634714457964	36.0	32.0	36.0	14.0	36.0
48	31.671592403814536	36.0	32.0	36.0	14.0	36.0
49	31.866826975775513	36.0	32.0	36.0	14.0	36.0
50	30.99389592239395	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	9.0
21	21.0
22	57.0
23	138.0
24	315.0
25	705.0
26	1280.0
27	2150.0
28	3532.0
29	4999.0
30	6701.0
31	9374.0
32	13378.0
33	18642.0
34	25897.0
35	58768.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.48669170253009	17.611907568323236	13.241139223157262	23.660261505989418
2	10.989312140312414	23.74965744039463	36.78268018635242	28.47835023294053
3	19.494683766304945	30.04494135700976	27.32174175161679	23.13863312506851
4	12.106078044502905	19.478241806423327	32.46876027622493	35.946919872848845
5	11.142168146443057	39.44837224597172	34.64800504220103	14.761454565384193
6	32.57037549583125	31.713332465557283	19.77159220919797	15.944699829413498
7	25.405568343746577	31.97550147977639	23.539405897182945	19.07952427929409
8	29.651704483174395	32.350926230406664	21.919187767181846	16.07818151923709
9	28.146580072344623	16.068590375972818	18.133426504439328	37.65140304724323
10	15.823331141072018	30.00383645730571	32.85788666008988	21.31494574153239
11	32.25432971610216	23.994300120574373	19.556341115861013	24.19502904746246
12	22.97626877123753	26.76477036062699	30.312123205086046	19.946837663049436
13	32.96544448098213	20.615477364901896	23.39759399320399	23.02148416091198
14	24.73076290693851	21.34508933464869	25.13153567905294	28.79261207935986
15	29.58730680697139	23.697659761043514	26.866162446563628	19.848870985421463
16	23.007782527677296	24.938342650443936	31.2198564068837	20.834018414995068
17	24.551956593225913	25.479557163213855	30.307327633453905	19.661158610106323
18	27.15115641784501	21.32179655814973	32.32763345390771	19.199413570097555
19	22.93036829990135	31.67680587526033	27.88487887756221	17.507946947276114
20	22.90639044174066	23.556532938726296	33.8533103145895	19.683766304943546
21	21.95138660528335	28.525430231283572	25.81593225912529	23.707250904307795
22	21.61569659103365	28.931683656691874	23.541461142168146	25.911158610106327
23	19.07061821769155	28.826866162446564	25.485037816507727	26.617477803354163
24	20.292257839100618	33.33767221358252	25.015243171401757	21.354826775915104
25	25.16784500712485	23.824399868464322	22.5193193028609	28.488435821549928
26	20.97871436694595	24.574047558694772	28.25775689025602	26.189481184103258
27	25.77568902560168	27.017065501106412	25.12417190186823	22.08307357142368
28	22.872293052539273	24.391129501873714	27.55759863530798	25.178978810279034
29	22.83993752055245	25.3076016661186	30.693713690671924	21.158747122657022
30	25.096768447662825	25.842142402049777	30.18010920276501	18.880979947522388
31	24.16368083196887	24.038310028979154	26.024375372515706	25.773633766536268
32	18.223982132947857	27.656936156802566	29.50529914295697	24.61378256729261
33	20.217444919434396	28.080127151156418	28.131508275786477	23.570919653622713
34	17.85677584659546	28.522885309693287	30.407557872669848	23.2127809710414
35	21.35003117142916	29.325806517911584	29.355265231182393	19.968897079476868
36	23.861394278197963	27.760194015126604	27.6656527458073	20.712758960868136
37	24.00284997876218	28.177794828932768	27.59615252867106	20.223202663633995
38	24.46084073221528	28.640523950454895	24.191603639153787	22.707031678176037
39	22.987545044736446	26.245152980831154	27.14056698135182	23.626734993080582
40	23.939493587635646	28.387728817275022	25.050696042968323	22.622081552121013
41	20.01411277891578	28.102242287640355	26.98486644241507	24.898778491028793
42	26.824238687356562	29.33511458226287	26.022676669064502	17.817970061316068
43	25.778097489124107	24.181139314219163	24.21813448429418	25.822628712362555
44	23.400975583020468	27.129292154229812	29.251733304102384	20.21799895864734
45	28.66264292614084	24.62611412168504	26.139469880178396	20.571773071995725
46	20.895563289578252	26.404455893233948	31.918829300375435	20.781151516812365
47	26.884659651430447	25.26033103145895	29.124191603639154	18.730817713471446
48	23.13636830747097	31.72952420100709	25.349227554550747	19.78487993697119
49	21.360061384999042	27.43690224986983	32.7786303472089	18.424406017922227
50	21.401412648064287	31.736625401631873	23.925955866737002	22.936006083566834
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	147.0
1	104.0
2	61.0
3	190.0
4	319.0
5	281.0
6	243.0
7	201.0
8	159.0
9	203.0
10	247.0
11	366.0
12	485.0
13	680.5
14	876.0
15	1018.0
16	1160.0
17	1191.5
18	1223.0
19	1110.5
20	998.0
21	1034.0
22	1070.0
23	1131.5
24	1193.0
25	1242.5
26	1292.0
27	1606.0
28	1920.0
29	2191.5
30	2463.0
31	2622.0
32	2781.0
33	3344.0
34	3907.0
35	4410.5
36	4914.0
37	5022.5
38	5131.0
39	5084.5
40	5038.0
41	5344.0
42	5650.0
43	6228.0
44	6806.0
45	10414.0
46	14022.0
47	18734.5
48	23447.0
49	22367.5
50	21288.0
51	15935.0
52	10582.0
53	8001.5
54	5421.0
55	5160.5
56	4900.0
57	4741.0
58	4582.0
59	4235.5
60	3889.0
61	3475.0
62	3061.0
63	2584.0
64	2107.0
65	1770.5
66	1434.0
67	1243.0
68	1052.0
69	918.0
70	784.0
71	617.5
72	451.0
73	384.0
74	317.0
75	252.0
76	187.0
77	164.5
78	142.0
79	117.5
80	93.0
81	71.5
82	50.0
83	45.5
84	41.0
85	30.5
86	20.0
87	13.0
88	6.0
89	5.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030143593116299466
2	0.00548065329387263
3	0.0
4	0.0
5	0.0
6	6.850816617340787E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	6.850816617340787E-4
25	0.0
26	6.850816617340787E-4
27	6.850816617340787E-4
28	6.850816617340787E-4
29	0.0
30	6.850816617340787E-4
31	6.850816617340787E-4
32	6.850816617340787E-4
33	0.0
34	6.850816617340787E-4
35	6.850816617340787E-4
36	0.0
37	0.0013701633234681574
38	0.0
39	0.0013701633234681574
40	0.0
41	6.850816617340787E-4
42	0.002055244985202236
43	0.002055244985202236
44	0.002740326646936315
45	6.850816617340787E-4
46	0.002740326646936315
47	0.0
48	0.002055244985202236
49	0.002740326646936315
50	6.850816617340787E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	145968.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.370821001863426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.55596996119147	45.97377507398882
2	9.911294793609194	10.777704702400525
3	2.367572198981906	3.8618053271950017
4	0.8908321153167684	1.9374109393839747
5	0.46494632326999646	1.2639756658993753
6	0.2948440098785343	0.9618546530746465
7	0.23310317020311475	0.8871807519456318
8	0.17010231339146212	0.739888194672805
9	0.11844161080590697	0.5795790858270305
>10	0.8454714984123785	8.48062589060616
>50	0.05796078826672043	2.092239394935876
>100	0.06552089108411874	7.156363038474186
>500	0.015120205634796633	5.747150060287186
>1k	0.00882011995363137	9.54044722130878
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2924	2.003178778910446	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2638	1.8072454236544997	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2475	1.6955771127918449	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2473	1.6942069494683767	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	1245	0.852926668858928	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1163	0.7967499725967336	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1008	0.6905623150279513	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	930	0.6371259454126932	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	837	0.5734133508714239	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	836	0.5727282692096898	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	746	0.5110709196536227	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	693	0.47476159158171655	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	675	0.4624301216705031	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	653	0.4473583251123534	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	638	0.4370821001863422	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	628	0.4302312835690015	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	611	0.41858489531952203	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	582	0.3987175271292338	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	560	0.3836457305710841	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	475	0.3254137893236874	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	424	0.29047462457524936	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	390	0.2671818480762907	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	382	0.26170119478241805	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	373	0.25553545982681136	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	332	0.2274471116957141	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	329	0.22539186671051192	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	317	0.21717088676970292	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	313	0.21443056012276662	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	310	0.2123753151375644	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	281	0.1925079469472761	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	267	0.182916803682999	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	242	0.16578976213964705	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	216	0.147977638934561	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	214	0.14660747561109283	No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA	210	0.14386714896415653	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	205	0.14044174065548615	No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA	195	0.13359092403814535	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG	189	0.12948043406774087	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	186	0.12742518908253864	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	184	0.1260550257590705	No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT	178	0.12194453578866601	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	177	0.12125945412693193	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	176	0.12057437246519787	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	174	0.11920420914172969	No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	171	0.11714896415652747	No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG	170	0.11646388249479336	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	167	0.11440863750959114	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	165	0.11303847418612299	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	162	0.11098322920092074	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	159	0.10892798421571852	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	159	0.10892798421571852	No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	159	0.10892798421571852	No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	155	0.1061876575687822	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	153	0.10481749424531406	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	150	0.10276224926011181	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	6.850816617340787E-4	0.0
19	0.0	0.0	0.0	6.850816617340787E-4	0.0
20	0.0	0.0	0.0	6.850816617340787E-4	0.0
21	0.0	0.0	0.0	6.850816617340787E-4	0.0
22	0.0	0.0	0.0	6.850816617340787E-4	0.0
23	0.0	0.0	0.0	0.0013701633234681574	0.0
24	0.0	0.0	0.0	0.0013701633234681574	0.0
25	0.0	0.0	0.0	0.0013701633234681574	0.0
26	0.0	0.0	0.0	0.002740326646936315	0.0
27	0.0	0.0	0.0	0.002740326646936315	0.0
28	0.0	0.0	0.0	0.0034254083086703935	0.0
29	0.0	0.0	0.0	0.00548065329387263	0.0
30	0.0	0.0	0.0	0.007535898279074866	0.0
31	0.0	0.0	0.0	0.011646388249479339	0.0
32	0.0	0.0	0.0	0.01575687821988381	0.0
33	0.0	0.0	0.0	0.023977858160692755	0.0
34	0.0	0.0	0.0	0.037679491395374326	0.0
35	0.0	0.0	0.0	0.05001096130658775	0.0
36	0.0	0.0	0.0	0.060287186232598926	0.0
37	0.0	0.0	0.0	0.08015455442288721	0.0
38	0.0	0.0	0.0	0.11920420914172969	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGA	25	0.0023460428	35.2	1
ATTATCT	25	0.0023460428	35.2	3
GTACTCA	40	1.8091841E-5	33.0	35
CGTTAGG	35	3.2041236E-4	31.428572	15
TCGTTAG	40	7.0023455E-4	27.500002	14
CTCGTTA	40	7.0023455E-4	27.500002	13
ACCTCTA	40	7.0023455E-4	27.500002	28
ATACATG	40	7.0023455E-4	27.500002	1
AAGCTCG	40	7.0023455E-4	27.500002	10
TATCACC	50	8.292202E-5	26.400002	3
CTATCAC	50	8.292202E-5	26.400002	2
AAAGTAC	185	0.0	26.162163	3
TATAGAA	60	9.676536E-6	25.666666	2
AAAAAGT	200	0.0	25.300001	1
CACCTCT	45	0.0013923387	24.444445	27
GTTCCAG	45	0.0013923387	24.444445	19
CTACCTA	45	0.0013923387	24.444445	32
TAGGCTT	45	0.0013923387	24.444445	18
CCGCCTG	45	0.0013923387	24.444445	9
TCCTAAA	85	2.3233406E-7	23.294117	2
>>END_MODULE