FastQCFastQC Report
Thu 2 Feb 2017
SRR4062092_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062092_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117943
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC11010.9335017762817633No Hit
GTATCAACGCAGAGTACTTTTTTTT10710.9080657605792628No Hit
CCCATGTACTCTGCGTTGATACCAC7110.6028335721492586No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7100.6019857049591751No Hit
GTATCAACGCAGAGTACATGGGAAG6500.5511136735541744No Hit
TATCAACGCAGAGTACTTTTTTTTT6050.512959650000424No Hit
GTCCTAAAGTGTGTATTTCTCATTT5150.4366516028929229No Hit
GTACATGGAAGCAGTGGTATCAACG4810.40782411843008914No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4330.36712649330608854No Hit
TCCATGTACTCTGCGTTGATACCAC3870.3281246025622546No Hit
GGTATCAACGCAGAGTACTTTTTTT3850.32642886818208794No Hit
GTCCTACAGTGGACATTTCTAAATT3810.3230373994217546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA3650.309471524380421No Hit
CTGTAGGACGTGGAATATGGCAAGA3630.30777579000025435No Hit
CTTTAGGACGTGAAATATGGCGAGG3620.306927922810171No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3590.304384321239921No Hit
TATCAACGCAGAGTACATGGGAAGC3530.2992971180994209No Hit
GAGTACATGGGAAGCAGTGGTATCA3520.29844925090933755No Hit
GCGTTGATACCACTGCTTCCCATGT3270.27725257115725394No Hit
CATGTACTCTGCGTTGATACCACTG3020.25605589140517027No Hit
GGTATCAACGCAGAGTACATGGGAA2810.23825068041342007No Hit
ACGCAGAGTACATGGGAAGCAGTGG2740.23231561008283663No Hit
AAAAAGTACTCTGCGTTGATACCAC2280.19331371933900274No Hit
GTCCTACAGTGTGCATTTCTCATTT2250.1907701177687527No Hit
GTCCTTCAGTGTGCATTTCTCATTT2150.18229144586791923No Hit
CTGAAGGACCTGGAATATGGCGAGA1930.16363836768608567No Hit
ACTCTGCGTTGATACCACTGCTTCC1890.16024689892575228No Hit
TTTCTAAATTTTCCACCTTTTTCAG1890.16024689892575228No Hit
CAGTGGTATCAACGCAGAGTACATG1850.1568554301654189No Hit
GAGTACATGGAAGCAGTGGTATCAA1830.1551596957852522No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1760.14922462545466877No Hit
GCTTCCCATGTACTCTGCGTTGATA1680.14244168793400203No Hit
GTACATGGGAGTGGTATCAACGCAA1670.14159382074391869No Hit
GTATCAACGCAGAGTACATGGGAGT1610.1365066176034186No Hit
ATTTAGAAATGTCCACTGTAGGACG1520.1288758128926685No Hit
CTGTAGGACCTGGAATATGGCGAGA1350.11446207066125162No Hit
AAAGTACTCTGCGTTGATACCACTG1220.10343979719016813No Hit
ATCAACGCAGAGTACTTTTTTTTTT1210.10259193000008479No Hit
GTGGTATCAACGCAGAGTACATGGG1200.10174406281000144No Hit
GTACTCTGCGTTGATACCACTGCTT1190.10089619561991808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAA350.002149689216.2908213
TGATTTC350.002155812216.28389218
CGAGAAA400.00522990614.25446614
TTAGAAA400.00524470114.2484053
TAGAAAT400.00524470114.2484054
GGCGAGA1102.6411726E-912.95309619
GGACCTG1207.075869E-1012.665256
AGGACCT1457.2759576E-1212.4468825
TAGGACC550.003042101212.0895564
ATTTCTC2250.011.40357315
GACCTGG1501.6734703E-1011.39872557
AAGGACC1001.882534E-611.3987254
ACAGTGT1104.827034E-711.2260166
TGGCGAG1950.011.20387518
CACTGTG600.005825966611.0820937
TACAGTG2500.011.0187665
TCTCATT2350.010.913671518
GTCCTAA1053.399513E-610.8513151
CGTGGAA1158.5672946E-710.7379299
ACGTGGA1158.5672946E-710.7379298
Produced by FastQC (version 0.11.5)