##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062092_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117943
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9989825593719	32.0	32.0	32.0	32.0	32.0
2	30.73288792043614	32.0	32.0	32.0	32.0	32.0
3	30.765030565612204	32.0	32.0	32.0	32.0	32.0
4	30.68920580280305	32.0	32.0	32.0	32.0	32.0
5	30.74891261032872	32.0	32.0	32.0	32.0	32.0
6	34.32170624793332	36.0	36.0	36.0	32.0	36.0
7	34.237063666347304	36.0	36.0	36.0	32.0	36.0
8	34.13648966025962	36.0	36.0	36.0	32.0	36.0
9	34.151624089602606	36.0	36.0	36.0	32.0	36.0
10	33.994437991233056	36.0	36.0	36.0	32.0	36.0
11	34.26594202284154	36.0	36.0	36.0	32.0	36.0
12	34.14162773543152	36.0	36.0	36.0	32.0	36.0
13	34.125560652179445	36.0	36.0	36.0	32.0	36.0
14	34.055009623292605	36.0	36.0	36.0	32.0	36.0
15	33.98515384550164	36.0	36.0	36.0	32.0	36.0
16	34.02532579296779	36.0	36.0	36.0	32.0	36.0
17	33.958522337061126	36.0	36.0	36.0	32.0	36.0
18	33.91990198655282	36.0	36.0	36.0	32.0	36.0
19	33.91678183529332	36.0	36.0	36.0	32.0	36.0
20	33.815538014125465	36.0	36.0	36.0	32.0	36.0
21	33.76559015795766	36.0	36.0	36.0	32.0	36.0
22	33.67184996142204	36.0	36.0	36.0	27.0	36.0
23	33.71974597898985	36.0	36.0	36.0	27.0	36.0
24	33.63757916959887	36.0	36.0	36.0	27.0	36.0
25	33.23150165758036	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	5.0
6	26.0
7	5.0
8	14.0
9	16.0
10	7.0
11	2.0
12	1.0
13	6.0
14	77.0
15	94.0
16	106.0
17	124.0
18	182.0
19	203.0
20	311.0
21	388.0
22	594.0
23	731.0
24	909.0
25	1220.0
26	1515.0
27	1763.0
28	2427.0
29	3041.0
30	4187.0
31	5460.0
32	7634.0
33	10689.0
34	23137.0
35	53068.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.01538057164175	17.528255653250355	11.828795753809109	24.627568021298785
2	13.582947550490921	20.96793339098879	41.204999067338186	24.244119991182107
3	19.303392316627523	27.50735524787397	27.413241989774722	25.77601044572378
4	11.891740645587975	17.31997049322107	35.6463934745928	35.14189538659815
5	12.247684635478508	38.74207008854361	34.207178478135496	14.803066797842385
6	31.869390386670965	35.277999287398835	18.5513836339266	14.301226692003596
7	27.38313473877177	32.06113996673117	22.02193027124283	18.533795023254235
8	26.52867242067028	35.07735655302934	20.227274656075224	18.166696370225154
9	28.116857213182932	14.685237525356692	19.33983483139392	37.85807043006646
10	16.00363352349905	28.479013854930724	32.7484039663135	22.768948655256725
11	33.09193099232493	22.374855668002443	24.23588942470964	20.297323914962984
12	26.13866933806488	24.546017947041797	30.480257405064904	18.835055309828423
13	30.903025364164204	21.992462055617807	24.330243455230722	22.774269124987267
14	22.36811950940033	22.202605780248692	27.14425158086831	28.28502312948266
15	24.334006825474983	30.004923850111208	24.1472400971187	21.51382922729511
16	21.170268756048287	26.75761022733061	30.0419347719054	22.03018624471571
17	21.83457268004482	27.9396285355336	28.429425146854097	21.796373637567484
18	22.380180440151754	24.690425468711542	32.26789336547194	20.66150072566477
19	24.272281344942886	25.723475397593226	28.921194222380635	21.083049035083253
20	24.86656200941915	25.0422164707879	29.530315244600956	20.56090627519199
21	22.75720443976783	25.54139370693459	27.542344115949902	24.15905773734768
22	24.054145803275905	28.041245862683528	26.162267673767293	21.742340660273275
23	20.198712020295435	28.848030273462356	28.70633553653094	22.246922169711265
24	23.130971979430086	27.632762512516763	28.777515656557085	20.458749851496073
25	22.445481544336022	26.531183708103523	28.21977089520577	22.80356385235469
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	422.0
1	422.0
2	330.5
3	239.0
4	239.0
5	239.0
6	572.5
7	906.0
8	906.0
9	906.0
10	824.5
11	743.0
12	743.0
13	743.0
14	599.5
15	456.0
16	456.0
17	456.0
18	797.0
19	1138.0
20	1138.0
21	1138.0
22	1830.0
23	2522.0
24	2522.0
25	2522.0
26	3814.0
27	5106.0
28	5106.0
29	5106.0
30	6637.5
31	8169.0
32	8169.0
33	8169.0
34	8458.0
35	8747.0
36	8747.0
37	8747.0
38	9560.0
39	10373.0
40	10373.0
41	10373.0
42	11621.0
43	12869.0
44	12869.0
45	12869.0
46	16292.0
47	19715.0
48	19715.0
49	19715.0
50	17621.5
51	15528.0
52	15528.0
53	15528.0
54	13094.0
55	10660.0
56	10660.0
57	10660.0
58	9330.0
59	8000.0
60	8000.0
61	8000.0
62	6823.0
63	5646.0
64	5646.0
65	5646.0
66	4594.0
67	3542.0
68	3542.0
69	3542.0
70	2657.0
71	1772.0
72	1772.0
73	1772.0
74	1264.0
75	756.0
76	756.0
77	756.0
78	556.5
79	357.0
80	357.0
81	357.0
82	249.5
83	142.0
84	142.0
85	142.0
86	101.0
87	60.0
88	60.0
89	60.0
90	41.5
91	23.0
92	23.0
93	23.0
94	17.0
95	11.0
96	11.0
97	11.0
98	26.0
99	41.0
100	41.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0016957343801666908
2	8.478671900833454E-4
3	0.0
4	0.0033914687603333817
5	0.029675351652917084
6	0.05511136735541745
7	0.09750472685958471
8	0.09496112528933469
9	0.10513553157033481
10	0.12802794570258513
11	0.1348108832232519
12	0.1288758128926685
13	0.117853539421585
14	0.10852700033066821
15	0.12633221132241845
16	0.11954927380175169
17	0.117853539421585
18	0.10174406281000144
19	0.09241752371908464
20	0.08309098462816784
21	0.0839388518182512
22	0.09580899247941803
23	0.07122084396700101
24	0.08563458619841788
25	0.07885164867775112
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	117943.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.84621384906268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.44829451075829	56.264466733930796
2	10.32822137237481	13.601485463317026
3	2.1078791156436307	4.163875770499309
4	0.6966173497637167	1.8347845993403593
5	0.3425142606971324	1.1276633628108494
6	0.21246185343995055	0.8393885181825119
7	0.1313400548537876	0.6053771737195086
8	0.07983415098955718	0.42054212628133925
9	0.07597120819973989	0.45021747793425637
>10	0.45969019198825667	5.803650916120499
>50	0.06051943704047076	2.8098318679362064
>100	0.047642961074413154	7.531604249510357
>500	0.006438237983028805	2.705544203555955
>1k	0.002575295193211522	1.841567536861026
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	1101	0.9335017762817633	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1071	0.9080657605792628	No Hit
CCCATGTACTCTGCGTTGATACCAC	711	0.6028335721492586	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	710	0.6019857049591751	No Hit
GTATCAACGCAGAGTACATGGGAAG	650	0.5511136735541744	No Hit
TATCAACGCAGAGTACTTTTTTTTT	605	0.512959650000424	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	515	0.4366516028929229	No Hit
GTACATGGAAGCAGTGGTATCAACG	481	0.40782411843008914	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	433	0.36712649330608854	No Hit
TCCATGTACTCTGCGTTGATACCAC	387	0.3281246025622546	No Hit
GGTATCAACGCAGAGTACTTTTTTT	385	0.32642886818208794	No Hit
GTCCTACAGTGGACATTTCTAAATT	381	0.3230373994217546	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	365	0.309471524380421	No Hit
CTGTAGGACGTGGAATATGGCAAGA	363	0.30777579000025435	No Hit
CTTTAGGACGTGAAATATGGCGAGG	362	0.306927922810171	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	359	0.304384321239921	No Hit
TATCAACGCAGAGTACATGGGAAGC	353	0.2992971180994209	No Hit
GAGTACATGGGAAGCAGTGGTATCA	352	0.29844925090933755	No Hit
GCGTTGATACCACTGCTTCCCATGT	327	0.27725257115725394	No Hit
CATGTACTCTGCGTTGATACCACTG	302	0.25605589140517027	No Hit
GGTATCAACGCAGAGTACATGGGAA	281	0.23825068041342007	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	274	0.23231561008283663	No Hit
AAAAAGTACTCTGCGTTGATACCAC	228	0.19331371933900274	No Hit
GTCCTACAGTGTGCATTTCTCATTT	225	0.1907701177687527	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	215	0.18229144586791923	No Hit
CTGAAGGACCTGGAATATGGCGAGA	193	0.16363836768608567	No Hit
ACTCTGCGTTGATACCACTGCTTCC	189	0.16024689892575228	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	189	0.16024689892575228	No Hit
CAGTGGTATCAACGCAGAGTACATG	185	0.1568554301654189	No Hit
GAGTACATGGAAGCAGTGGTATCAA	183	0.1551596957852522	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	176	0.14922462545466877	No Hit
GCTTCCCATGTACTCTGCGTTGATA	168	0.14244168793400203	No Hit
GTACATGGGAGTGGTATCAACGCAA	167	0.14159382074391869	No Hit
GTATCAACGCAGAGTACATGGGAGT	161	0.1365066176034186	No Hit
ATTTAGAAATGTCCACTGTAGGACG	152	0.1288758128926685	No Hit
CTGTAGGACCTGGAATATGGCGAGA	135	0.11446207066125162	No Hit
AAAGTACTCTGCGTTGATACCACTG	122	0.10343979719016813	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	121	0.10259193000008479	No Hit
GTGGTATCAACGCAGAGTACATGGG	120	0.10174406281000144	No Hit
GTACTCTGCGTTGATACCACTGCTT	119	0.10089619561991808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAA	35	0.0021496892	16.29082	13
TGATTTC	35	0.0021558122	16.283892	18
CGAGAAA	40	0.005229906	14.254466	14
TTAGAAA	40	0.005244701	14.248405	3
TAGAAAT	40	0.005244701	14.248405	4
GGCGAGA	110	2.6411726E-9	12.953096	19
GGACCTG	120	7.075869E-10	12.66525	6
AGGACCT	145	7.2759576E-12	12.446882	5
TAGGACC	55	0.0030421012	12.089556	4
ATTTCTC	225	0.0	11.403573	15
GACCTGG	150	1.6734703E-10	11.3987255	7
AAGGACC	100	1.882534E-6	11.398725	4
ACAGTGT	110	4.827034E-7	11.226016	6
TGGCGAG	195	0.0	11.203875	18
CACTGTG	60	0.0058259666	11.082093	7
TACAGTG	250	0.0	11.018766	5
TCTCATT	235	0.0	10.9136715	18
GTCCTAA	105	3.399513E-6	10.851315	1
CGTGGAA	115	8.5672946E-7	10.737929	9
ACGTGGA	115	8.5672946E-7	10.737929	8
>>END_MODULE