FastQCFastQC Report
Thu 2 Feb 2017
SRR4062092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117943
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.4256293294218394No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3020.25605589140517027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.24503361793408682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.22892414132250324No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2210.18737864900841933No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA2170.18398718024808594No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2030.17211703958691912No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA2030.17211703958691912No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA1930.16363836768608567No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1810.1534639614050855No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1740.14752889107450207No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC1500.1271800785125018No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.12294074256208506No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1280.10852700033066821No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1180.10004832842983476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAAGG353.2018617E-431.4261478
GTAAATT300.005714568329.34351739
CTTCCAC300.00572643629.3310743
ATAGATT300.00572643629.3310744
TAGGACC554.914342E-627.997844
TGTAGAA406.997456E-427.497882
TAGAACA651.7965256E-523.6904814
TTCGTCA1407.2759576E-1221.99830244
GTCCTAA1650.021.3407381
GTAGAAC550.004462059619.9984573
TTTCGTC1504.5110937E-1019.06519743
TTAGGAC1901.8189894E-1218.5248873
GTCCACT951.5783306E-518.52488711
GATTTCG1556.9485395E-1018.45801741
ATTTCGT1556.9485395E-1018.45801742
GTCCTAC2050.018.2502351
ATGGAAT851.4178513E-418.11625110
AGTGTGC1601.0677468E-917.8736238
TGATTTC1852.1827873E-1117.8440340
TTTCTCG1002.4450339E-517.6061141
Produced by FastQC (version 0.11.5)