##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062092_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117943
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95880213323385	32.0	32.0	32.0	32.0	32.0
2	31.407756289054884	32.0	32.0	32.0	32.0	32.0
3	31.559660174830213	32.0	32.0	32.0	32.0	32.0
4	31.687857693970816	32.0	32.0	32.0	32.0	32.0
5	31.676063861356756	32.0	32.0	32.0	32.0	32.0
6	35.307606216562235	36.0	36.0	36.0	36.0	36.0
7	35.27648949068618	36.0	36.0	36.0	36.0	36.0
8	35.27176687043742	36.0	36.0	36.0	36.0	36.0
9	35.29142891057545	36.0	36.0	36.0	36.0	36.0
10	35.22432870115225	36.0	36.0	36.0	36.0	36.0
11	35.29786422254818	36.0	36.0	36.0	36.0	36.0
12	35.227652340537375	36.0	36.0	36.0	36.0	36.0
13	35.25168089670434	36.0	36.0	36.0	36.0	36.0
14	35.204954935858844	36.0	36.0	36.0	36.0	36.0
15	35.170039764971214	36.0	36.0	36.0	36.0	36.0
16	35.186691876584455	36.0	36.0	36.0	36.0	36.0
17	35.16835250926295	36.0	36.0	36.0	36.0	36.0
18	35.11483513222489	36.0	36.0	36.0	36.0	36.0
19	35.1007690155414	36.0	36.0	36.0	36.0	36.0
20	35.050846595389295	36.0	36.0	36.0	36.0	36.0
21	35.00970807932645	36.0	36.0	36.0	36.0	36.0
22	34.91785014795283	36.0	36.0	36.0	36.0	36.0
23	34.91359385465861	36.0	36.0	36.0	36.0	36.0
24	34.89055730310404	36.0	36.0	36.0	36.0	36.0
25	34.79979312040562	36.0	36.0	36.0	32.0	36.0
26	34.74731862001136	36.0	36.0	36.0	32.0	36.0
27	34.67164647329642	36.0	36.0	36.0	32.0	36.0
28	34.62289410986663	36.0	36.0	36.0	32.0	36.0
29	34.51798750243762	36.0	36.0	36.0	32.0	36.0
30	34.48475958725825	36.0	36.0	36.0	32.0	36.0
31	34.387619443290404	36.0	36.0	36.0	32.0	36.0
32	34.38482996023503	36.0	36.0	36.0	32.0	36.0
33	34.33734091891846	36.0	36.0	36.0	32.0	36.0
34	34.28426443281924	36.0	36.0	36.0	32.0	36.0
35	34.18339367321503	36.0	36.0	36.0	32.0	36.0
36	34.05138075171905	36.0	36.0	36.0	32.0	36.0
37	33.91973241311481	36.0	36.0	36.0	32.0	36.0
38	33.88273148893957	36.0	36.0	36.0	32.0	36.0
39	33.61759493992861	36.0	36.0	36.0	27.0	36.0
40	33.57392130096741	36.0	36.0	36.0	21.0	36.0
41	33.53992182664508	36.0	36.0	36.0	21.0	36.0
42	33.41603995150199	36.0	36.0	36.0	21.0	36.0
43	33.38676309742842	36.0	36.0	36.0	21.0	36.0
44	33.34116479994574	36.0	36.0	36.0	21.0	36.0
45	33.141509034024914	36.0	36.0	36.0	14.0	36.0
46	33.024350745699195	36.0	36.0	36.0	14.0	36.0
47	32.949984314456984	36.0	36.0	36.0	14.0	36.0
48	32.878933043928	36.0	36.0	36.0	14.0	36.0
49	32.83323300238251	36.0	36.0	36.0	14.0	36.0
50	32.46144323953096	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	8.0
21	10.0
22	33.0
23	81.0
24	183.0
25	479.0
26	983.0
27	1676.0
28	2500.0
29	3248.0
30	3963.0
31	5116.0
32	6790.0
33	9157.0
34	17706.0
35	66007.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.990364225490694	18.510696048993164	12.276282083905881	25.222657641610258
2	13.225705249412822	21.447043760651873	40.64966889101809	24.67758209891722
3	18.6632525881146	27.562466615229393	28.356918172337487	25.417362624318528
4	11.854031184555252	17.412648482741663	35.60279117878976	35.13052915391333
5	11.88370653620817	38.85944905589989	34.368296550028404	14.888547857863543
6	31.940852784819786	35.358605428045756	18.246101930593593	14.454439856540871
7	27.202123059443966	31.69666703407578	22.306537903902733	18.794672002577517
8	26.89095580068338	35.11611541168192	20.1343021629092	17.858626624725503
9	27.900765624072644	14.179730886953868	19.55351313770211	38.36599035127138
10	15.977209329930558	28.371331914568902	32.97016355358097	22.68129520191957
11	33.366965398539975	21.837667347786642	24.38889972274743	20.406467530925955
12	26.17874736101337	24.266806847375427	30.69872735134768	18.855718440263516
13	31.17014150903403	21.66978964415014	24.823855591260184	22.336213255555652
14	22.303994302332484	21.99960998109256	27.211449598534887	28.48494611804007
15	24.421118675970597	29.71604927804109	24.784005833326265	21.07882621266205
16	21.156161503111697	26.062810533991282	30.40307947974428	22.377948483152736
17	21.55532757348889	27.55737941208889	29.035211924404162	21.85208109001806
18	22.686382405060073	23.716541041011336	33.16093367134972	20.436142882578874
19	24.23713149572251	25.213874498698523	29.399794816140002	21.149199189438967
20	25.00720693222092	24.440826847094336	30.513303149005445	20.0386630716793
21	22.66518572530799	24.841660802251937	28.58160297770957	23.911550494730506
22	24.07773246398684	26.94691503522888	27.224167606386136	21.751184894398143
23	20.201283670925786	28.100862280932315	29.549867308784748	22.147986739357147
24	23.231561008283663	26.496697557294624	29.638893363743502	20.632848070678207
25	22.56174592811782	25.38599153828544	29.220894839032415	22.83136769456432
26	20.879577422992462	26.387322689773875	30.5096529679591	22.223446919274565
27	22.00893652018348	26.940132097708215	28.72065319688324	22.330278185225065
28	21.17923368463893	26.34113667002993	29.330767078454485	23.14886256687666
29	23.042486624895076	25.10873896712819	29.401490550520165	22.44728385745657
30	21.67675637177596	25.620220108188768	30.837191161757477	21.8658323582778
31	23.636841525143502	24.601714387458347	29.185284417048912	22.576159670349234
32	20.656265897914196	27.001865355265387	29.909275903001525	22.43259284381889
33	20.047989689847554	27.89337131810551	30.81429855352631	21.244340438520627
34	20.59656441301657	27.13706737209815	30.71848874870699	21.54787946617829
35	20.629631513794916	27.86369571484289	30.807515558494853	20.69915721286734
36	21.462910049769803	26.790907472253544	30.77588326564527	20.97029921233138
37	21.168749682036324	27.593311740066817	28.453085519510253	22.78485305838661
38	22.298907099191982	28.752872150106406	27.824457576965145	21.123763173736464
39	23.249563344694852	26.384154923606516	28.383442709128214	21.982839022570417
40	23.166275234647244	26.521285705807042	29.288724214239082	21.023714845306632
41	22.81525821795274	26.158398548451373	29.177653612338162	21.848689621257726
42	22.63439036798372	28.08207563167712	29.39545531626251	19.88807868407665
43	21.472481070401994	27.790260902007002	28.789947175186754	21.94731085240425
44	20.615567237578432	28.497541122604712	28.976598270306937	21.91029336950992
45	21.9654061387146	27.998982533491606	28.350856367644568	21.684754960149228
46	21.0964990376381	27.102146024639858	29.309219172623134	22.492135765098908
47	23.590208829688915	27.23773348142747	28.210237148453064	20.961820540430548
48	20.947446963658873	28.719327104071628	27.611965609048823	22.721260323220672
49	22.12791575163011	27.86318118995735	28.245588746534168	21.763314311878375
50	21.030497782827297	30.6113970307691	26.466174338451626	21.891930847951976
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	226.0
1	157.0
2	88.0
3	203.0
4	318.0
5	292.5
6	267.0
7	217.0
8	167.0
9	215.5
10	264.0
11	360.0
12	456.0
13	751.5
14	1047.0
15	1274.0
16	1501.0
17	1384.5
18	1268.0
19	1140.0
20	1012.0
21	1004.0
22	996.0
23	957.0
24	918.0
25	923.0
26	928.0
27	1175.0
28	1422.0
29	1813.5
30	2205.0
31	2933.0
32	3661.0
33	4540.0
34	5419.0
35	6266.5
36	7114.0
37	6907.5
38	6701.0
39	6343.5
40	5986.0
41	6045.5
42	6105.0
43	6275.5
44	6446.0
45	6956.0
46	7466.0
47	7951.0
48	8436.0
49	8374.0
50	8312.0
51	7856.0
52	7400.0
53	6829.0
54	6258.0
55	6016.0
56	5774.0
57	5405.5
58	5037.0
59	4695.5
60	4354.0
61	3849.0
62	3344.0
63	2876.5
64	2409.0
65	2047.5
66	1686.0
67	1405.5
68	1125.0
69	889.5
70	654.0
71	545.5
72	437.0
73	358.0
74	279.0
75	226.0
76	173.0
77	146.5
78	120.0
79	97.0
80	74.0
81	59.5
82	45.0
83	32.0
84	19.0
85	17.0
86	15.0
87	12.0
88	9.0
89	4.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04154549231408392
2	0.005087203140500072
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.478671900833454E-4
17	0.0
18	0.0
19	0.0
20	8.478671900833454E-4
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0016957343801666908
29	0.0
30	8.478671900833454E-4
31	0.0
32	0.002543601570250036
33	8.478671900833454E-4
34	8.478671900833454E-4
35	8.478671900833454E-4
36	0.0
37	0.0042393359504167265
38	0.0
39	8.478671900833454E-4
40	0.0
41	0.0
42	0.002543601570250036
43	0.005087203140500072
44	0.002543601570250036
45	0.002543601570250036
46	0.0033914687603333817
47	0.0
48	0.0042393359504167265
49	0.005087203140500072
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	117943.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.32585231849282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.21654947178992	65.80551622393868
2	10.012108285844414	15.283653968442385
3	2.0728496684107043	4.746360530086567
4	0.6387398495906511	1.9500945371916945
5	0.31548194310216504	1.2039714099183503
6	0.17107119449906133	0.7834292836370111
7	0.10330922784683573	0.5519615407442579
8	0.08220304151253596	0.5019373765293405
9	0.04887748414258895	0.3357554072730048
>10	0.29104320103087056	4.24272741917706
>50	0.027771297808289176	1.5261609421500215
>100	0.018884482509636643	2.6428020314897873
>500	0.001110851912331567	0.4256293294218394
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	502	0.4256293294218394	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	302	0.25605589140517027	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	289	0.24503361793408682	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	270	0.22892414132250324	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	221	0.18737864900841933	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	217	0.18398718024808594	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	203	0.17211703958691912	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	203	0.17211703958691912	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	193	0.16363836768608567	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	181	0.1534639614050855	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	174	0.14752889107450207	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	150	0.1271800785125018	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	145	0.12294074256208506	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	128	0.10852700033066821	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	118	0.10004832842983476	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	8.478671900833453E-4	0.0
14	0.0	0.0	0.0	0.0016957343801666906	0.0
15	0.0	0.0	0.0	0.0016957343801666906	0.0
16	0.0	0.0	0.0	0.0016957343801666906	0.0
17	0.0	0.0	0.0	0.0016957343801666906	0.0
18	0.0	0.0	0.0	0.0016957343801666906	0.0
19	0.0	0.0	0.0	0.0016957343801666906	0.0
20	0.0	0.0	0.0	0.0033914687603333812	0.0
21	0.0	0.0	0.0	0.0033914687603333812	8.478671900833453E-4
22	0.0	0.0	0.0	0.0042393359504167265	8.478671900833453E-4
23	0.0	0.0	0.0	0.008478671900833453	8.478671900833453E-4
24	0.0	0.0	0.0	0.013565875041333525	8.478671900833453E-4
25	0.0	0.0	0.0	0.01441374223141687	8.478671900833453E-4
26	0.0	0.0	0.0	0.017805210991750253	0.0016957343801666906
27	0.0	0.0	0.0	0.022044546942166978	0.0016957343801666906
28	0.0	0.0	0.0	0.032218953223167125	0.0016957343801666906
29	0.0	0.0	0.0	0.043241226694250616	0.0016957343801666906
30	0.0	0.0	0.0	0.06019857049591752	0.0016957343801666906
31	0.0	0.0	0.0	0.07969951586783446	0.0016957343801666906
32	0.0	0.0	0.0	0.09580899247941803	0.0016957343801666906
33	0.0	0.0	0.0	0.12209287537200172	0.0016957343801666906
34	0.0	0.0	0.0	0.14837675826458543	0.0016957343801666906
35	0.0	0.0	0.0	0.19331371933900274	0.0016957343801666906
36	0.0	0.0	0.0	0.23570707884317	0.0016957343801666906
37	0.0	0.0	0.0	0.2933620477688375	0.0016957343801666906
38	0.0	0.0	0.0	0.39171464181850557	0.0016957343801666906
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCAAGG	35	3.2018617E-4	31.426147	8
GTAAATT	30	0.0057145683	29.343517	39
CTTCCAC	30	0.005726436	29.331074	3
ATAGATT	30	0.005726436	29.331074	4
TAGGACC	55	4.914342E-6	27.99784	4
TGTAGAA	40	6.997456E-4	27.49788	2
TAGAACA	65	1.7965256E-5	23.690481	4
TTCGTCA	140	7.2759576E-12	21.998302	44
GTCCTAA	165	0.0	21.340738	1
GTAGAAC	55	0.0044620596	19.998457	3
TTTCGTC	150	4.5110937E-10	19.065197	43
TTAGGAC	190	1.8189894E-12	18.524887	3
GTCCACT	95	1.5783306E-5	18.524887	11
GATTTCG	155	6.9485395E-10	18.458017	41
ATTTCGT	155	6.9485395E-10	18.458017	42
GTCCTAC	205	0.0	18.250235	1
ATGGAAT	85	1.4178513E-4	18.116251	10
AGTGTGC	160	1.0677468E-9	17.873623	8
TGATTTC	185	2.1827873E-11	17.84403	40
TTTCTCG	100	2.4450339E-5	17.60611	41
>>END_MODULE