FastQCFastQC Report
Thu 2 Feb 2017
SRR4062091_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062091_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382418
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA136150.984868541931601No Hit
GTCCTACAGTGGACATTTCTAAATT133770.96765233091583No Hit
GTCCTAAAGTGTGTATTTCTCATTT122040.8828010051952448No Hit
CTTTAGGACGTGAAATATGGCGAGG109350.7910053254514915No Hit
GTCCTACAGTGTGCATTTCTCATTT90020.6511778637141588No Hit
CTGTAGGACCTGGAATATGGCGAGA69030.4993424564784313No Hit
GATATACACTGTTCTACAAATCCCG58970.42657141327731557No Hit
TTGTAGAACAGTGTATATCAATGAG50540.3655913045113706No Hit
CTGAAGGACCTGGAATATGGCGAGA47440.3431668279782237No Hit
GTGTATATCAATGAGTTACAATGAA46750.33817557352407157No Hit
ATTTAGAAATGTCCACTGTAGGACG46240.33448638544926357No Hit
GTCCTTCAGTGTGCATTTCTCATTT43950.3179212076231646No Hit
GTGTATATCAATGAGTTACAATGAG40640.2939776536474496No Hit
GAATATGGCAAGAAAACTGAAAATC36120.2612813201216998No Hit
TTTCTAAATTTTCCACCTTTTTCAG35170.2544093031196064No Hit
GTTCTACAGTGTGGTTTTTATCATT30010.21708340024507783No Hit
CTGTAGGACATGGAATATGGCAAGA30000.21701106322400315No Hit
GGAATATGGCGAGAAAACTGAAAAT29730.21505796365498714No Hit
ATTCCAGGTCCTTCAGTGTGCATTT27620.19979485220823226No Hit
GATATACACTGTTCTACAATGCCGG26820.19400789052225884No Hit
GTTCTACAAATCCCGTTTCCAACGA26740.1934291943536615No Hit
TTCCAGGTCCTTCAGTGTGCATTTC24840.1796851603494746No Hit
GAAATATGGCGAGGAAAACTGAAAA24290.1757066241903679No Hit
GCCATATTCCACGTCCTACAGTGGA24000.17360885057920253No Hit
ACCTGGAATATGGCGAGAAAACTGA23440.16955797739902112No Hit
GACCTGGAATATGGCGAGAAAACTG22940.16594112634528776No Hit
ACAGTGGACATTTCTAAATTTTCCA22390.16196259018618103No Hit
GTATCAACGCAGAGTACTTTTTTTT21860.15812872806922365No Hit
CTGTAGAACATATTAGATGAGTGAG21540.15581394339483426No Hit
CCATATTCCAGGTCCTTCAGTGTGC21280.15393318084689292No Hit
GTCCACTGTAGGACGTGGAATATGG20950.15154605915142888No Hit
CCATATTTCACGTCCTAAAGTGTGT20840.15075035191960753No Hit
ATACACACTTTAGGACGTGAAATAT19480.14091251705345273No Hit
CCACTGTAGGACGTGGAATATGGCA19360.1400444728005567No Hit
CCTAAAGTGTGTATTTCTCATTTTC18920.13686164387327132No Hit
GTCCTACAGTGGACATTTCTAAATA17910.12955560474472988No Hit
CACTTTAGGACGTGAAATATGGCGA16830.12174320646866578No Hit
GTAGGACGTGGAATATGGCAAGAAA16770.12130918434221777No Hit
ATGTTCTACAGTGTGGTTTTTATCA16670.1205858141314711No Hit
TCCTAAAGTGTGTATTTCTCATTTT16150.11682428903558838No Hit
CTACAGTGGACATTTCTAAATTTTC15900.11501586350872169No Hit
TTGATATACACTGTTCTACAAATCC15880.11487118946657233No Hit
GAAATACACACTTTAGGACGTGAAA15840.11458184138227367No Hit
GTGTATTTCTCATTTTCCGTGATTT15530.11233939372895897No Hit
ATTCCACGTCCTACAGTGGACATTT15270.1104586311810176No Hit
GTAGAACAGTGTATATCAATGAGTT15250.11031395713886827No Hit
GTAGAACATATTAGATGAGTGAGTT15160.10966292394919627No Hit
AGTGTGTATTTCTCATTTTCCGTGA14000.10127182950453481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGTG307.715104E-419.0025737
GTCGATA402.7572247E-416.6278539
AAGGCGT350.002168598416.2873296
CGACGGG350.002169648216.28614814
AATGGCG702.5034751E-816.2849716
CCAACGT2000.015.19930419
CTATGAC508.7216074E-515.1982023
GGCGATA653.3730648E-614.61471619
TAGGACC24250.014.5333564
TAAGCTC602.5654506E-514.25141212
TGCACCG400.005276504414.2508965
AGCTTCG400.005280310314.24934919
TAGAAAT12100.014.21009354
AGGACGG551.9588569E-413.8190515
GGTATCA8800.013.8110471
CACCTTT9950.013.4626714
ACCTTTT10250.013.439383515
CCACCTT10050.013.42421613
TCCAACG7450.013.38864918
AAATGTC12400.013.3324497
Produced by FastQC (version 0.11.5)