##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062091_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1382418
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.394471860175432	32.0	32.0	32.0	32.0	32.0
2	31.068902459314042	32.0	32.0	32.0	32.0	32.0
3	31.06986960528581	32.0	32.0	32.0	32.0	32.0
4	31.061259329667294	32.0	32.0	32.0	32.0	32.0
5	31.06777834200654	32.0	32.0	32.0	32.0	32.0
6	34.769008360712895	36.0	36.0	36.0	36.0	36.0
7	34.686686660619294	36.0	36.0	36.0	32.0	36.0
8	34.68072753682316	36.0	36.0	36.0	32.0	36.0
9	34.72340420914658	36.0	36.0	36.0	32.0	36.0
10	34.46943905533637	36.0	36.0	36.0	32.0	36.0
11	34.69133069737229	36.0	36.0	36.0	32.0	36.0
12	34.53951771461309	36.0	36.0	36.0	32.0	36.0
13	34.588235974936666	36.0	36.0	36.0	32.0	36.0
14	34.56493405033789	36.0	36.0	36.0	32.0	36.0
15	34.5185175540249	36.0	36.0	36.0	32.0	36.0
16	34.50867176208643	36.0	36.0	36.0	32.0	36.0
17	34.47231228181346	36.0	36.0	36.0	32.0	36.0
18	34.47707784476186	36.0	36.0	36.0	32.0	36.0
19	34.34567475249888	36.0	36.0	36.0	32.0	36.0
20	34.298887890638	36.0	36.0	36.0	32.0	36.0
21	34.2423666358511	36.0	36.0	36.0	32.0	36.0
22	34.14587049647791	36.0	36.0	36.0	32.0	36.0
23	34.16595993397077	36.0	36.0	36.0	32.0	36.0
24	34.09375528964467	36.0	36.0	36.0	32.0	36.0
25	33.74060595275814	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	18.0
5	101.0
6	310.0
7	44.0
8	228.0
9	184.0
10	117.0
11	20.0
12	51.0
13	65.0
14	151.0
15	231.0
16	377.0
17	483.0
18	726.0
19	891.0
20	1418.0
21	2062.0
22	3161.0
23	4542.0
24	6469.0
25	9291.0
26	12666.0
27	16355.0
28	21914.0
29	29874.0
30	39558.0
31	54960.0
32	79605.0
33	117254.0
34	278815.0
35	700475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93621658611817	19.981741858525446	12.625606103022506	25.45643545233388
2	10.93853080404162	20.30799701392345	50.110241779609034	18.643230402425896
3	20.35145817599963	25.497970214378928	29.804139431660026	24.346432177961418
4	10.08429563372925	17.63928818949589	40.98249411478733	31.293922061987526
5	9.862871946277643	41.623972444135696	37.47814634711126	11.035009262475397
6	29.398365132871863	40.5407381295516	18.831518060917006	11.229378676659532
7	26.263299942719637	31.531210556387123	22.034546359076185	20.170943141817055
8	20.86559747530449	46.43690180235046	19.641847204651395	13.055653517693653
9	26.17826792912504	14.65058611035068	20.780302404841258	38.39084355568302
10	15.408300961837249	29.88214433425782	35.606013963625614	19.103540740279314
11	34.129179095720254	20.326518903992895	29.307237512151378	16.237064488135474
12	25.904950090576495	23.40944269923026	35.308970513566194	15.376636696627049
13	31.8241441775536	23.594501354815296	25.938067448869397	18.64328701876171
14	17.590642580286325	27.591670963667646	26.93379528507946	27.883891170966564
15	19.400175289916483	41.558269410460895	21.889147236286462	17.152408063336157
16	17.0215400927306	27.963736281245154	37.18508604594235	17.829637580081897
17	17.0832947052484	32.61844819394389	32.01121469875888	18.287042402048833
18	17.19929174828821	27.95617239918457	39.413628414073784	15.430907438453435
19	24.55362670383578	25.0908169360824	26.807667939829482	23.547888420252338
20	23.651777646486412	30.31396978753968	30.07518361483747	15.959068951136443
21	20.600341600133802	27.69683458748417	26.02829387458395	25.674529937798074
22	22.011377229899406	34.47129769356208	27.275663213140582	16.241661863397933
23	18.844598180124127	33.425106584145546	29.99674944599411	17.733545789736212
24	23.83334081493463	27.852263956685874	31.86677054025367	16.44762468812583
25	20.32268690971355	31.25776942764161	31.77622600391684	16.643317658728005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	733.0
1	733.0
2	614.5
3	496.0
4	496.0
5	496.0
6	1079.5
7	1663.0
8	1663.0
9	1663.0
10	2033.0
11	2403.0
12	2403.0
13	2403.0
14	3596.5
15	4790.0
16	4790.0
17	4790.0
18	9264.5
19	13739.0
20	13739.0
21	13739.0
22	27536.0
23	41333.0
24	41333.0
25	41333.0
26	72064.0
27	102795.0
28	102795.0
29	102795.0
30	147189.5
31	191584.0
32	191584.0
33	191584.0
34	196349.5
35	201115.0
36	201115.0
37	201115.0
38	196847.0
39	192579.0
40	192579.0
41	192579.0
42	188135.5
43	183692.0
44	183692.0
45	183692.0
46	178990.5
47	174289.0
48	174289.0
49	174289.0
50	147764.0
51	121239.0
52	121239.0
53	121239.0
54	90996.0
55	60753.0
56	60753.0
57	60753.0
58	49142.0
59	37531.0
60	37531.0
61	37531.0
62	30242.0
63	22953.0
64	22953.0
65	22953.0
66	18247.5
67	13542.0
68	13542.0
69	13542.0
70	10345.5
71	7149.0
72	7149.0
73	7149.0
74	5705.0
75	4261.0
76	4261.0
77	4261.0
78	3122.5
79	1984.0
80	1984.0
81	1984.0
82	1383.0
83	782.0
84	782.0
85	782.0
86	555.0
87	328.0
88	328.0
89	328.0
90	222.5
91	117.0
92	117.0
93	117.0
94	85.5
95	54.0
96	54.0
97	54.0
98	284.0
99	514.0
100	514.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0015190774425680221
2	1.4467404214933544E-4
3	4.340221264480063E-4
4	0.01012718295045348
5	0.03602383649518452
6	0.06503098194612628
7	0.10785449842232955
8	0.10843319459092692
9	0.1171859741409617
10	0.1324490855877166
11	0.13859773237906334
12	0.13143636729267125
13	0.1292662566604312
14	0.11646260393021503
15	0.13367881494598594
16	0.11892206264675373
17	0.12514304645917515
18	0.11233939372895897
19	0.09714861930327875
20	0.09157866868052933
21	0.09194035378590266
22	0.10228454779958014
23	0.0806557784982545
24	0.09056595038548398
25	0.08702143635282526
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1382418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.7421806545413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.01167336538649	23.97616023332453
2	15.802577528534542	10.980320066074837
3	6.1004841834145225	6.358323707410777
4	2.863345613117257	3.9791548226923226
5	1.580672751844307	2.745800915014292
6	0.94281591942153	1.965328859791215
7	0.6385604324392841	1.5529487331853336
8	0.4398950179264548	1.2226329745466862
9	0.3359951963134286	1.0505885228441285
>10	1.9102968060161238	12.64550638823666
>50	0.19540684388007049	4.729878221083847
>100	0.1461657501839397	10.29669678484028
>500	0.018557419775760985	4.421728464518349
>1k	0.011885089069869395	8.497913181993782
>5k	8.340413382364487E-4	1.9454764927850192
>10k+	8.340413382364487E-4	3.631541631657946
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGA	13615	0.984868541931601	No Hit
GTCCTACAGTGGACATTTCTAAATT	13377	0.96765233091583	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	12204	0.8828010051952448	No Hit
CTTTAGGACGTGAAATATGGCGAGG	10935	0.7910053254514915	No Hit
GTCCTACAGTGTGCATTTCTCATTT	9002	0.6511778637141588	No Hit
CTGTAGGACCTGGAATATGGCGAGA	6903	0.4993424564784313	No Hit
GATATACACTGTTCTACAAATCCCG	5897	0.42657141327731557	No Hit
TTGTAGAACAGTGTATATCAATGAG	5054	0.3655913045113706	No Hit
CTGAAGGACCTGGAATATGGCGAGA	4744	0.3431668279782237	No Hit
GTGTATATCAATGAGTTACAATGAA	4675	0.33817557352407157	No Hit
ATTTAGAAATGTCCACTGTAGGACG	4624	0.33448638544926357	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	4395	0.3179212076231646	No Hit
GTGTATATCAATGAGTTACAATGAG	4064	0.2939776536474496	No Hit
GAATATGGCAAGAAAACTGAAAATC	3612	0.2612813201216998	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	3517	0.2544093031196064	No Hit
GTTCTACAGTGTGGTTTTTATCATT	3001	0.21708340024507783	No Hit
CTGTAGGACATGGAATATGGCAAGA	3000	0.21701106322400315	No Hit
GGAATATGGCGAGAAAACTGAAAAT	2973	0.21505796365498714	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	2762	0.19979485220823226	No Hit
GATATACACTGTTCTACAATGCCGG	2682	0.19400789052225884	No Hit
GTTCTACAAATCCCGTTTCCAACGA	2674	0.1934291943536615	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	2484	0.1796851603494746	No Hit
GAAATATGGCGAGGAAAACTGAAAA	2429	0.1757066241903679	No Hit
GCCATATTCCACGTCCTACAGTGGA	2400	0.17360885057920253	No Hit
ACCTGGAATATGGCGAGAAAACTGA	2344	0.16955797739902112	No Hit
GACCTGGAATATGGCGAGAAAACTG	2294	0.16594112634528776	No Hit
ACAGTGGACATTTCTAAATTTTCCA	2239	0.16196259018618103	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2186	0.15812872806922365	No Hit
CTGTAGAACATATTAGATGAGTGAG	2154	0.15581394339483426	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	2128	0.15393318084689292	No Hit
GTCCACTGTAGGACGTGGAATATGG	2095	0.15154605915142888	No Hit
CCATATTTCACGTCCTAAAGTGTGT	2084	0.15075035191960753	No Hit
ATACACACTTTAGGACGTGAAATAT	1948	0.14091251705345273	No Hit
CCACTGTAGGACGTGGAATATGGCA	1936	0.1400444728005567	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	1892	0.13686164387327132	No Hit
GTCCTACAGTGGACATTTCTAAATA	1791	0.12955560474472988	No Hit
CACTTTAGGACGTGAAATATGGCGA	1683	0.12174320646866578	No Hit
GTAGGACGTGGAATATGGCAAGAAA	1677	0.12130918434221777	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	1667	0.1205858141314711	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	1615	0.11682428903558838	No Hit
CTACAGTGGACATTTCTAAATTTTC	1590	0.11501586350872169	No Hit
TTGATATACACTGTTCTACAAATCC	1588	0.11487118946657233	No Hit
GAAATACACACTTTAGGACGTGAAA	1584	0.11458184138227367	No Hit
GTGTATTTCTCATTTTCCGTGATTT	1553	0.11233939372895897	No Hit
ATTCCACGTCCTACAGTGGACATTT	1527	0.1104586311810176	No Hit
GTAGAACAGTGTATATCAATGAGTT	1525	0.11031395713886827	No Hit
GTAGAACATATTAGATGAGTGAGTT	1516	0.10966292394919627	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	1400	0.10127182950453481	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.233702107466772E-5	0.0
5	0.0	0.0	0.0	7.233702107466772E-5	0.0
6	0.0	0.0	0.0	7.233702107466772E-5	0.0
7	0.0	0.0	0.0	1.4467404214933544E-4	0.0
8	0.0	0.0	0.0	1.4467404214933544E-4	0.0
9	0.0	0.0	0.0	1.4467404214933544E-4	0.0
10	0.0	0.0	0.0	1.4467404214933544E-4	0.0
11	0.0	0.0	0.0	2.1701106322400315E-4	0.0
12	0.0	0.0	0.0	2.893480842986709E-4	0.0
13	0.0	0.0	0.0	2.893480842986709E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCGTG	30	7.715104E-4	19.002573	7
GTCGATA	40	2.7572247E-4	16.627853	9
AAGGCGT	35	0.0021685984	16.287329	6
CGACGGG	35	0.0021696482	16.286148	14
AATGGCG	70	2.5034751E-8	16.28497	16
CCAACGT	200	0.0	15.199304	19
CTATGAC	50	8.7216074E-5	15.198202	3
GGCGATA	65	3.3730648E-6	14.614716	19
TAGGACC	2425	0.0	14.533356	4
TAAGCTC	60	2.5654506E-5	14.251412	12
TGCACCG	40	0.0052765044	14.250896	5
AGCTTCG	40	0.0052803103	14.249349	19
TAGAAAT	1210	0.0	14.2100935	4
AGGACGG	55	1.9588569E-4	13.819051	5
GGTATCA	880	0.0	13.811047	1
CACCTTT	995	0.0	13.46267	14
ACCTTTT	1025	0.0	13.4393835	15
CCACCTT	1005	0.0	13.424216	13
TCCAACG	745	0.0	13.388649	18
AAATGTC	1240	0.0	13.332449	7
>>END_MODULE