FastQCFastQC Report
Thu 2 Feb 2017
SRR4062091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382418
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA68150.4929767986238605No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC51400.37181228832379204No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA45310.3277590424893194No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA40920.2960030902375403No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG34500.24956272270760363No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA25940.18764223266768806No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC25180.1821446190660133No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC23030.16659215953495976No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA22330.161528568059733No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA21410.1548735621208636No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA21130.1528481255307729No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA18160.13136403027159657No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA16130.11667961499343903No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT15240.1102416201177936No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA14580.10546737672686554No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA14540.10517802864256685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC28300.029.7726524
GGTATCA10300.028.6367781
GTGTAGG3800.027.2251851
TTCGTAA858.22547E-925.88599244
GTATCAA26150.025.4209521
TGTAGGA79200.025.0017072
AGGACCT47000.024.6670915
CTGTAGG76550.024.5854131
TACCGTC450.001398643424.443467
GACGTGT1001.7462298E-924.1990247
GGACCTG45850.024.086276
GGCGATA551.5942146E-423.99903319
GTAGGAC82000.023.662463
TGTGGCG1153.5106495E-1022.95559716
CCTACAC4450.022.7406563
TAGGACA17100.022.5137144
GGACCTA2350.022.4671826
ACTGTTC24350.022.3153218
GTCCTAC77250.022.1400761
GATATAC26100.022.096191
Produced by FastQC (version 0.11.5)