##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062091_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1382418
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.115962755114587	32.0	32.0	32.0	32.0	32.0
2	31.54060421666963	32.0	32.0	32.0	32.0	32.0
3	31.658107026962902	32.0	32.0	32.0	32.0	32.0
4	31.713794235896813	32.0	32.0	32.0	32.0	32.0
5	31.738291168083748	32.0	32.0	32.0	32.0	32.0
6	35.387348833710206	36.0	36.0	36.0	36.0	36.0
7	35.412707299818145	36.0	36.0	36.0	36.0	36.0
8	35.36732160605548	36.0	36.0	36.0	36.0	36.0
9	35.45320879791785	36.0	36.0	36.0	36.0	36.0
10	35.34828322547884	36.0	36.0	36.0	36.0	36.0
11	35.43358882769177	36.0	36.0	36.0	36.0	36.0
12	35.385175829597124	36.0	36.0	36.0	36.0	36.0
13	35.39213754450535	36.0	36.0	36.0	36.0	36.0
14	35.374053289236684	36.0	36.0	36.0	36.0	36.0
15	35.340610437653446	36.0	36.0	36.0	36.0	36.0
16	35.36969353697651	36.0	36.0	36.0	36.0	36.0
17	35.37528663544601	36.0	36.0	36.0	36.0	36.0
18	35.348236929785344	36.0	36.0	36.0	36.0	36.0
19	35.28627954786468	36.0	36.0	36.0	36.0	36.0
20	35.250317921207625	36.0	36.0	36.0	36.0	36.0
21	35.209310063960395	36.0	36.0	36.0	36.0	36.0
22	35.1249491832427	36.0	36.0	36.0	36.0	36.0
23	35.138785808633855	36.0	36.0	36.0	36.0	36.0
24	35.13601240724586	36.0	36.0	36.0	36.0	36.0
25	35.03015079375413	36.0	36.0	36.0	36.0	36.0
26	35.05410592165322	36.0	36.0	36.0	36.0	36.0
27	34.95948692797692	36.0	36.0	36.0	36.0	36.0
28	34.85194998907711	36.0	36.0	36.0	36.0	36.0
29	34.76558537287564	36.0	36.0	36.0	32.0	36.0
30	34.676530542860405	36.0	36.0	36.0	32.0	36.0
31	34.67668823756635	36.0	36.0	36.0	32.0	36.0
32	34.598568595026975	36.0	36.0	36.0	32.0	36.0
33	34.689509251181626	36.0	36.0	36.0	32.0	36.0
34	34.64696640234719	36.0	36.0	36.0	32.0	36.0
35	34.62826077206749	36.0	36.0	36.0	32.0	36.0
36	34.484581363957936	36.0	36.0	36.0	32.0	36.0
37	34.3096979350674	36.0	36.0	36.0	32.0	36.0
38	34.270565776776635	36.0	36.0	36.0	32.0	36.0
39	33.92098048491845	36.0	36.0	36.0	32.0	36.0
40	34.153797910617484	36.0	36.0	36.0	32.0	36.0
41	34.1593454367637	36.0	36.0	36.0	32.0	36.0
42	34.15102667934011	36.0	36.0	36.0	32.0	36.0
43	34.222471061574716	36.0	36.0	36.0	32.0	36.0
44	34.02632271859886	36.0	36.0	36.0	32.0	36.0
45	33.993457839814006	36.0	36.0	36.0	32.0	36.0
46	33.68607396605079	36.0	36.0	36.0	27.0	36.0
47	33.74034336937164	36.0	36.0	36.0	27.0	36.0
48	33.66914999660016	36.0	36.0	36.0	27.0	36.0
49	33.56842792845579	36.0	36.0	36.0	21.0	36.0
50	33.3743368503593	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	7.0
20	11.0
21	29.0
22	106.0
23	268.0
24	762.0
25	1934.0
26	4707.0
27	9208.0
28	15846.0
29	24216.0
30	34175.0
31	48419.0
32	70534.0
33	116816.0
34	246019.0
35	809360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.63506306488845	20.549239820248786	12.997011382796275	25.818685732066488
2	10.681840870520116	20.777214966441107	49.64777269945441	18.89317146358437
3	20.206711168595177	25.70977393136055	30.118228140380726	23.965286759663545
4	10.036761674110146	18.07825129591773	41.07324991428063	30.81173711569149
5	9.60252948278269	41.80750092048926	37.366293961952145	11.223675634775903
6	29.636129263737203	40.70813808309233	18.542419275267758	11.113313377902712
7	26.39064306164995	31.533443574953452	22.117622889748255	19.958290473648347
8	21.03061447405922	46.47328087452565	19.40621432880648	13.089890322608646
9	25.986062102779332	14.491203094867108	20.858307689859362	38.664427112494195
10	15.423844307582801	30.1455131515938	35.36180807830917	19.068834462514232
11	33.95998894690318	20.228758595446532	29.62555464410909	16.1856978135412
12	25.959080393918484	23.39393728958969	35.25525564626618	15.391726670225648
13	31.938603229992662	23.479656659563172	26.143829145743187	18.43791096470098
14	17.588384989200083	27.51823254616187	27.0761086733535	27.817273791284546
15	19.33004344561486	41.659035110943286	21.896850301428366	17.114071142013486
16	17.151339218220922	28.00469614318752	36.887510299035526	17.956454339556036
17	17.248400990149147	32.8122897705325	31.63898328870139	18.300325950616962
18	17.27734829649809	27.901060244484842	39.376179546403144	15.44541191261392
19	24.752209534308726	24.892688029235728	27.0769767176064	23.278125718849147
20	23.856925431781555	30.259647630373575	29.95069487244034	15.932732065404526
21	20.66829328632388	27.31751707335775	26.381185995253244	25.633003645065127
22	22.261645898707915	34.08513199336235	27.492914588785737	16.160307519143995
23	18.886183484300698	33.31127054190556	29.965176958054656	17.83736901573909
24	24.10856184276487	27.682778007249702	31.71178261589799	16.49687753408743
25	20.535614176081967	31.18076869936842	31.51055437123349	16.773062753316122
26	15.088555365549489	33.76750201821165	33.81632971936008	17.32761289687879
27	17.191172247367465	32.17193414703131	32.02906801368332	18.6078255919179
28	16.6064809590361	33.771632855609276	27.89448403776653	21.727402147588087
29	19.382483318962272	28.389013952465554	27.24130647392087	24.987196254651302
30	16.912676912170543	30.263491983116257	30.91525938678887	21.90857171792433
31	23.083600379048182	27.32192330784644	29.512445656498432	20.08203065660694
32	18.7667868678137	32.43745501499199	30.12149116937511	18.6742669478192
33	17.304030925657475	32.71817663619818	33.29217338969859	16.68561904844576
34	18.719857871295687	32.573572654691915	32.84368084987872	15.862888624133667
35	17.00748328106972	33.85647580502237	32.07538980696824	17.060651106939666
36	15.67720257665028	31.880513449289833	33.856114119132094	18.58616985492779
37	17.17409263007432	30.995108514021247	28.491133548707246	23.339665307197183
38	19.356952817454633	34.494197847539596	27.581238091517907	18.56761124348786
39	22.50153355594007	28.09814468666269	29.838730678641905	19.56159107875533
40	24.976255355656072	28.129934744726086	30.662455684500404	16.231354215117435
41	23.206513286627292	26.03281961509109	33.8348023914844	16.92586470679721
42	19.8630875260337	33.83089435834666	30.965614178246643	15.340403937372995
43	16.271617048550375	33.83298682396311	32.95072622287425	16.944669904612265
44	15.806740275777356	33.86841364722461	30.788289770947525	19.536556306050507
45	16.43719576991326	33.93748331685213	30.174199190817596	19.451121722417014
46	18.310166849322005	28.3314706105752	30.61759206009903	22.74077048000376
47	23.19945254590028	28.792917240246847	29.41249234121263	18.595137872640244
48	17.630412184318637	30.37283152737298	28.928280261319895	23.068476026988492
49	21.783062770053885	29.010108054107352	29.391918041787868	19.8149111340509
50	18.018728366349706	34.80893666426745	26.861208265365544	20.311126704017305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	399.0
1	218.0
2	37.0
3	87.0
4	137.0
5	145.5
6	154.0
7	134.0
8	114.0
9	167.0
10	220.0
11	375.5
12	531.0
13	945.5
14	1360.0
15	1927.5
16	2495.0
17	2856.0
18	3217.0
19	3529.5
20	3842.0
21	4781.0
22	5720.0
23	7322.0
24	8924.0
25	14038.5
26	19153.0
27	32042.0
28	44931.0
29	56501.0
30	68071.0
31	82033.5
32	95996.0
33	120578.5
34	145161.0
35	170417.5
36	195674.0
37	190312.0
38	184950.0
39	161170.5
40	137391.0
41	115372.5
42	93354.0
43	78666.5
44	63979.0
45	58068.0
46	52157.0
47	49273.0
48	46389.0
49	44279.0
50	42169.0
51	38646.0
52	35123.0
53	32367.5
54	29612.0
55	27647.0
56	25682.0
57	23159.0
58	20636.0
59	18455.5
60	16275.0
61	13756.0
62	11237.0
63	9472.0
64	7707.0
65	6786.5
66	5866.0
67	5379.0
68	4892.0
69	4221.0
70	3550.0
71	2692.5
72	1835.0
73	1629.5
74	1424.0
75	1071.5
76	719.0
77	604.5
78	490.0
79	421.0
80	352.0
81	279.0
82	206.0
83	172.0
84	138.0
85	106.0
86	74.0
87	51.0
88	28.0
89	21.5
90	15.0
91	11.0
92	7.0
93	7.5
94	8.0
95	5.5
96	3.0
97	4.0
98	5.0
99	7.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0367472067059312
2	0.005208265517376076
3	4.340221264480063E-4
4	0.0
5	7.233702107466772E-5
6	2.893480842986709E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.06359147522674E-4
17	0.0
18	7.233702107466772E-5
19	0.0
20	4.340221264480063E-4
21	7.233702107466772E-5
22	0.0
23	0.0
24	0.0012297293582693514
25	3.6168510537333857E-4
26	4.340221264480063E-4
27	3.6168510537333857E-4
28	0.002170110632240032
29	7.233702107466771E-4
30	0.0016637514847173574
31	5.786961685973418E-4
32	9.403812739706805E-4
33	4.340221264480063E-4
34	6.510331896720095E-4
35	2.1701106322400315E-4
36	2.1701106322400315E-4
37	0.0011573923371946836
38	0.0
39	7.233702107466771E-4
40	7.233702107466772E-5
41	9.403812739706805E-4
42	0.005425276580600079
43	0.004340221264480064
44	0.0051359284963014085
45	0.00253179573761337
46	0.0023871216954640347
47	0.0013744034004186867
48	0.0036891880748080538
49	0.003978536159106725
50	0.0016637514847173574
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1382418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.51222224226277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.68312513591181	33.34141447341462
2	14.86324222395094	13.826448511219802
3	5.60322830644384	7.818558007903606
4	2.609093238718173	4.854188982801793
5	1.4327434807603272	3.332004159663874
6	0.8580148818918578	2.3944907324233764
7	0.5712897154841811	1.860036794792351
8	0.39700874943648823	1.4772607348730205
9	0.28784702372981824	1.2049564265545674
>10	1.483658108311013	12.57438302736168
>50	0.1225759439378716	3.95029736808451
>100	0.0778550728683573	7.16388515321901
>500	0.006409740314651665	2.13461986477673
>1k	0.0035957079813899585	3.198148620132516
>5k	3.1267025925130074E-4	0.8693071427785056
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	6815	0.4929767986238605	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	5140	0.37181228832379204	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	4531	0.3277590424893194	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	4092	0.2960030902375403	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	3450	0.24956272270760363	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	2594	0.18764223266768806	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	2518	0.1821446190660133	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	2303	0.16659215953495976	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	2233	0.161528568059733	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	2141	0.1548735621208636	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	2113	0.1528481255307729	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	1816	0.13136403027159657	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1613	0.11667961499343903	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	1524	0.1102416201177936	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1458	0.10546737672686554	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	1454	0.10517802864256685	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.4467404214933544E-4	0.0
13	0.0	0.0	0.0	1.4467404214933544E-4	0.0
14	0.0	0.0	0.0	1.4467404214933544E-4	0.0
15	0.0	0.0	0.0	2.1701106322400315E-4	0.0
16	0.0	0.0	0.0	3.616851053733386E-4	0.0
17	0.0	0.0	0.0	3.616851053733386E-4	0.0
18	0.0	0.0	0.0	3.616851053733386E-4	0.0
19	0.0	0.0	0.0	4.340221264480063E-4	0.0
20	0.0	0.0	0.0	7.957072318213449E-4	0.0
21	0.0	0.0	0.0	0.0011573923371946836	0.0
22	0.0	0.0	0.0	0.0015190774425680221	0.0
23	0.0	0.0	0.0	0.0015190774425680221	0.0
24	0.0	0.0	0.0	0.0022424476533146995	0.0
25	0.0	0.0	0.0	0.0031828289272853795	0.0
26	0.0	0.0	0.0	0.004050873180181392	0.0
27	0.0	0.0	0.0	0.004846580412002737	0.0
28	0.0	0.0	0.0	0.006293320833496091	0.0
29	0.0	0.0	0.0	0.00853576848681079	0.0
30	0.0	0.0	0.0	0.011067564224424162	0.0
31	0.0	0.0	0.0	0.016348166762874906	0.0
32	0.0	0.0	0.0	0.022062791427773655	0.0
33	0.0	0.0	0.0	0.026258338650104383	0.0
34	0.0	0.0	0.0	0.03161127820962979	0.0
35	0.0	0.0	0.0	0.03768758797990188	0.0
36	0.0	0.0	0.0	0.04672971561423535	0.0
37	0.0	0.0	0.0	0.05866532409155552	0.0
38	0.0	0.0	0.0	0.0757368610651771	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	2830	0.0	29.772652	4
GGTATCA	1030	0.0	28.636778	1
GTGTAGG	380	0.0	27.225185	1
TTCGTAA	85	8.22547E-9	25.885992	44
GTATCAA	2615	0.0	25.420952	1
TGTAGGA	7920	0.0	25.001707	2
AGGACCT	4700	0.0	24.667091	5
CTGTAGG	7655	0.0	24.585413	1
TACCGTC	45	0.0013986434	24.44346	7
GACGTGT	100	1.7462298E-9	24.199024	7
GGACCTG	4585	0.0	24.08627	6
GGCGATA	55	1.5942146E-4	23.999033	19
GTAGGAC	8200	0.0	23.66246	3
TGTGGCG	115	3.5106495E-10	22.955597	16
CCTACAC	445	0.0	22.740656	3
TAGGACA	1710	0.0	22.513714	4
GGACCTA	235	0.0	22.467182	6
ACTGTTC	2435	0.0	22.315321	8
GTCCTAC	7725	0.0	22.140076	1
GATATAC	2610	0.0	22.09619	1
>>END_MODULE