FastQCFastQC Report
Thu 2 Feb 2017
SRR4062090_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062090_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969474
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22590.23301295341597608No Hit
TATCAACGCAGAGTACTTTTTTTTT14870.15338214330657657No Hit
GCGCAAGACGGACCAGAGCGAAAGC13230.13646575359421706No Hit
GATTAAGAGGGACGGCCGGGGGCAT11120.11470137414721798No Hit
GAATAGGACCGCGGTTCTATTTTGT11110.11459822542945969No Hit
GTCCTAAAGTGTGTATTTCTCATTT10860.11201950748550246No Hit
ATCAGATACCGTCGTAGTTCCGACC10440.1076872613396543No Hit
GTCCTACAGTGGACATTTCTAAATT10410.10737781518637941No Hit
GTATCTGATCGTCTTCGAACCTCCG10270.10593373313776337No Hit
TCGTAGTTCCGACCATAAACGATGC9960.10273612288725639No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG9900.10211723058070667No Hit
GAATAACGCCGCCGCATCGCCAGTC9720.10026055366105745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA601.4665802E-615.83212916
GCGTTAT1152.5465852E-1114.0402351
GATAACG759.642026E-713.93587311
GTCTAAC551.9627946E-413.8149891
TCCAACG1205.0931703E-1113.45661418
ACTCTAA1650.013.24552210
TCCTATA655.449594E-513.1535242
CTAAACT957.377821E-812.9996824
GTCCTAT2050.012.972611
CGCCAGT2300.012.80271218
TCGCCAG2300.012.8013917
ACGAACG751.4768055E-512.66701115
GTCGGCG853.9377737E-612.29445212
CGTTATT1251.4078978E-912.1597022
CGCGCAG550.003061639612.09373710
TCGAACG550.003067570512.0906133
TAGTACT550.003067570512.0906134
TAGAAAT1501.4551915E-1112.0330384
GGTATCA6650.011.9972271
CCGTCGT2000.011.8765519
Produced by FastQC (version 0.11.5)