##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062090_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	969474
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.311269822604835	32.0	32.0	32.0	32.0	32.0
2	30.946036716817574	32.0	32.0	32.0	32.0	32.0
3	30.970667599131076	32.0	32.0	32.0	32.0	32.0
4	30.972434536666274	32.0	32.0	32.0	32.0	32.0
5	30.93978281005989	32.0	32.0	32.0	32.0	32.0
6	34.58975279378302	36.0	36.0	36.0	32.0	36.0
7	34.51390547864099	36.0	36.0	36.0	32.0	36.0
8	34.47784984434858	36.0	36.0	36.0	32.0	36.0
9	34.57481582796444	36.0	36.0	36.0	32.0	36.0
10	34.34494684746574	36.0	36.0	36.0	32.0	36.0
11	34.57020817474218	36.0	36.0	36.0	32.0	36.0
12	34.422364086092045	36.0	36.0	36.0	32.0	36.0
13	34.481669441367174	36.0	36.0	36.0	32.0	36.0
14	34.421193348145486	36.0	36.0	36.0	32.0	36.0
15	34.389082120820156	36.0	36.0	36.0	32.0	36.0
16	34.37659803150987	36.0	36.0	36.0	32.0	36.0
17	34.32122367386851	36.0	36.0	36.0	32.0	36.0
18	34.32535065406602	36.0	36.0	36.0	32.0	36.0
19	34.31263757460231	36.0	36.0	36.0	32.0	36.0
20	34.29109393341131	36.0	36.0	36.0	32.0	36.0
21	34.253214629788935	36.0	36.0	36.0	32.0	36.0
22	34.227812195066605	36.0	36.0	36.0	32.0	36.0
23	34.20645009561886	36.0	36.0	36.0	32.0	36.0
24	34.18844961288286	36.0	36.0	36.0	32.0	36.0
25	33.84340580562243	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	17.0
5	86.0
6	207.0
7	39.0
8	202.0
9	138.0
10	71.0
11	22.0
12	38.0
13	31.0
14	194.0
15	260.0
16	413.0
17	462.0
18	679.0
19	845.0
20	1208.0
21	1648.0
22	2533.0
23	3430.0
24	4733.0
25	6895.0
26	9538.0
27	12126.0
28	16308.0
29	21986.0
30	29008.0
31	40483.0
32	58492.0
33	84643.0
34	197664.0
35	475075.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.68455347113414	18.002881995983362	11.812160702736659	25.500403830145846
2	16.149289356175984	19.680898797594157	38.44841475729597	25.721397088933884
3	19.180003878415793	23.355489815032573	28.507387556886872	28.95711874966476
4	12.561083676254711	15.74769927179283	35.695562155509734	35.99565489644273
5	14.705809516537444	36.68543256144145	33.3553461131278	15.253411808893299
6	34.24854924515331	35.42411999925682	16.73730344362029	13.59002731196958
7	30.12550832212908	30.33317430518122	20.338358209879985	19.20295916280972
8	27.566140188904104	34.28322396888638	19.203013047312776	18.94762279489674
9	26.97053009474904	14.342488757975797	19.07601611993696	39.61096502733821
10	16.85273828978004	26.900192751470424	30.752743041424008	25.49432591732553
11	37.04413040783439	21.044171728440975	22.585430354117598	19.32626750960704
12	24.554908709286906	23.73610187208971	29.039598560503084	22.66939085812031
13	29.74484879220716	19.694248226811172	25.111473076553626	25.44942990442804
14	23.014114006803972	20.610209262407334	24.991686100192304	31.383990630596394
15	24.665845828392467	28.75275276929624	21.772907378867824	24.808494023443473
16	24.87198342549425	26.10711873369454	24.904620164133874	24.116277676677335
17	23.35618730478756	26.62150270198807	25.695534696170945	24.32677529705343
18	24.019592656161898	25.40787052240887	27.34720475213824	23.225332069291
19	25.63038486002462	24.968300056997943	25.252153944009848	24.149161138967592
20	25.626162859131167	24.941095570758623	25.172067013726124	24.260674556384085
21	26.30414940672611	24.50419401068017	24.45876405747302	24.7328925251207
22	25.69416764936092	25.247773190723915	25.103931534262813	23.954127625652347
23	23.923589710235152	25.2368076827381	25.861102421710218	24.97850018531653
24	24.589766172138688	25.283908756851858	26.01594370042115	24.110381370588303
25	24.51373839388868	25.174511105627616	25.841375315802896	24.470375184680808
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	959.0
1	959.0
2	698.0
3	437.0
4	437.0
5	437.0
6	916.5
7	1396.0
8	1396.0
9	1396.0
10	1603.5
11	1811.0
12	1811.0
13	1811.0
14	2301.5
15	2792.0
16	2792.0
17	2792.0
18	4604.0
19	6416.0
20	6416.0
21	6416.0
22	10658.0
23	14900.0
24	14900.0
25	14900.0
26	23196.0
27	31492.0
28	31492.0
29	31492.0
30	40566.0
31	49640.0
32	49640.0
33	49640.0
34	58005.5
35	66371.0
36	66371.0
37	66371.0
38	74122.0
39	81873.0
40	81873.0
41	81873.0
42	92405.5
43	102938.0
44	102938.0
45	102938.0
46	113032.0
47	123126.0
48	123126.0
49	123126.0
50	124718.0
51	126310.0
52	126310.0
53	126310.0
54	116262.0
55	106214.0
56	106214.0
57	106214.0
58	98295.0
59	90376.0
60	90376.0
61	90376.0
62	80091.0
63	69806.0
64	69806.0
65	69806.0
66	57118.5
67	44431.0
68	44431.0
69	44431.0
70	33685.5
71	22940.0
72	22940.0
73	22940.0
74	17564.0
75	12188.0
76	12188.0
77	12188.0
78	9988.5
79	7789.0
80	7789.0
81	7789.0
82	5339.0
83	2889.0
84	2889.0
85	2889.0
86	2150.0
87	1411.0
88	1411.0
89	1411.0
90	952.0
91	493.0
92	493.0
93	493.0
94	294.5
95	96.0
96	96.0
97	96.0
98	238.0
99	380.0
100	380.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.220410243080268E-4
2	1.0314871775828954E-4
3	6.188923065497373E-4
4	0.01103691280013698
5	0.03507056403781844
6	0.06890334346253742
7	0.11263839979205219
8	0.11882732285754957
9	0.12707922027821272
10	0.1431704202485059
11	0.1479152612653872
12	0.1400759587157572
13	0.13440277923905128
14	0.1254288407940801
15	0.1400759587157572
16	0.12790441002027902
17	0.13388703565025983
18	0.11882732285754957
19	0.10500539467793875
20	0.0986101741769248
21	0.09799128187037508
22	0.10809985621068743
23	0.0888110459898873
24	0.09706294341055047
25	0.09396848187780178
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	969474.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.16037165415753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.34294629817589	38.54573134133911
2	14.806139973840729	15.1497524765034
3	4.592025715506763	7.047892267523241
4	1.898920308411201	3.8859787487977795
5	0.9095661713272989	2.326687168457687
6	0.5411191258267993	1.6610313351883135
7	0.3463050421272414	1.2401966262656847
8	0.22004084507922564	0.9005897130678469
9	0.1720321194759427	0.7921104451957496
>10	0.947030586712098	9.235178547281963
>50	0.1128536455739319	4.0654802154138165
>100	0.09762468157936834	9.901599982041125
>500	0.011568829132201221	4.054694690626289
>1k	0.001826657231400193	1.1930764422980376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2259	0.23301295341597608	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1487	0.15338214330657657	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1323	0.13646575359421706	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1112	0.11470137414721798	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1111	0.11459822542945969	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1086	0.11201950748550246	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1044	0.1076872613396543	No Hit
GTCCTACAGTGGACATTTCTAAATT	1041	0.10737781518637941	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1027	0.10593373313776337	No Hit
TCGTAGTTCCGACCATAAACGATGC	996	0.10273612288725639	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	990	0.10211723058070667	No Hit
GAATAACGCCGCCGCATCGCCAGTC	972	0.10026055366105745	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	2.062974355165791E-4
11	0.0	0.0	0.0	0.0	2.062974355165791E-4
12	0.0	0.0	0.0	0.0	3.0944615327486866E-4
13	0.0	0.0	0.0	0.0	4.125948710331582E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	60	1.4665802E-6	15.832129	16
GCGTTAT	115	2.5465852E-11	14.040235	1
GATAACG	75	9.642026E-7	13.935873	11
GTCTAAC	55	1.9627946E-4	13.814989	1
TCCAACG	120	5.0931703E-11	13.456614	18
ACTCTAA	165	0.0	13.245522	10
TCCTATA	65	5.449594E-5	13.153524	2
CTAAACT	95	7.377821E-8	12.999682	4
GTCCTAT	205	0.0	12.97261	1
CGCCAGT	230	0.0	12.802712	18
TCGCCAG	230	0.0	12.80139	17
ACGAACG	75	1.4768055E-5	12.667011	15
GTCGGCG	85	3.9377737E-6	12.294452	12
CGTTATT	125	1.4078978E-9	12.159702	2
CGCGCAG	55	0.0030616396	12.093737	10
TCGAACG	55	0.0030675705	12.090613	3
TAGTACT	55	0.0030675705	12.090613	4
TAGAAAT	150	1.4551915E-11	12.033038	4
GGTATCA	665	0.0	11.997227	1
CCGTCGT	200	0.0	11.876551	9
>>END_MODULE