FastQCFastQC Report
Thu 2 Feb 2017
SRR4062090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969474
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12500.12893589719786194No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12230.1261508818183881No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11970.12346901515667258No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10980.11325729209860193No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10780.11119431774343613No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10560.10892504595275378No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10270.10593373313776337No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCG300.005746005629.3317178
CGTACTC300.005746005629.3317173
GTATCAA16100.025.1544461
GTAGGAC7850.022.1389063
GTATAGG1407.2759576E-1222.010141
GGTTTCG703.214593E-521.99992224
GATATAC4750.020.8517111
TGTAGGA8400.020.6914712
CTGTAGG8400.020.4379881
GTCCTAC8450.020.317051
GATTTCG3150.020.25703241
TTAACGG550.004483073519.9999335
ATTTCGT3150.019.55851442
TATACTG1252.2879249E-819.3589345
TACTAGA708.113483E-418.85999542
TAGGACC7850.018.7760354
GGTATCA7900.018.6668281
CGTCGTA2750.018.39898510
GGTATAG600.00739139718.3417841
CCTACAG9600.018.3323233
Produced by FastQC (version 0.11.5)