##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062090_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	969474
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.274889269851485	32.0	32.0	32.0	32.0	32.0
2	31.485027963617384	32.0	32.0	32.0	32.0	32.0
3	31.60249062893899	32.0	32.0	32.0	32.0	32.0
4	31.675781918854966	32.0	32.0	32.0	32.0	32.0
5	31.65230527069318	32.0	32.0	32.0	32.0	32.0
6	35.31539473982799	36.0	36.0	36.0	36.0	36.0
7	35.32010554176801	36.0	36.0	36.0	36.0	36.0
8	35.27107792473032	36.0	36.0	36.0	36.0	36.0
9	35.34546774849042	36.0	36.0	36.0	36.0	36.0
10	35.229406874243146	36.0	36.0	36.0	36.0	36.0
11	35.34346769485308	36.0	36.0	36.0	36.0	36.0
12	35.26677456022544	36.0	36.0	36.0	36.0	36.0
13	35.296529870837176	36.0	36.0	36.0	36.0	36.0
14	35.26642901202095	36.0	36.0	36.0	36.0	36.0
15	35.24886484836107	36.0	36.0	36.0	36.0	36.0
16	35.253476627532045	36.0	36.0	36.0	36.0	36.0
17	35.24014362427461	36.0	36.0	36.0	36.0	36.0
18	35.23232907741724	36.0	36.0	36.0	36.0	36.0
19	35.21350753088788	36.0	36.0	36.0	36.0	36.0
20	35.20936610986989	36.0	36.0	36.0	36.0	36.0
21	35.19096541010899	36.0	36.0	36.0	36.0	36.0
22	35.17766850890276	36.0	36.0	36.0	36.0	36.0
23	35.15230320771882	36.0	36.0	36.0	36.0	36.0
24	35.13405001062432	36.0	36.0	36.0	36.0	36.0
25	35.10784404739065	36.0	36.0	36.0	36.0	36.0
26	35.07886750959799	36.0	36.0	36.0	36.0	36.0
27	35.06002017588919	36.0	36.0	36.0	36.0	36.0
28	35.03291166137514	36.0	36.0	36.0	36.0	36.0
29	35.00244875055958	36.0	36.0	36.0	36.0	36.0
30	34.9757600513268	36.0	36.0	36.0	36.0	36.0
31	34.971396860565626	36.0	36.0	36.0	36.0	36.0
32	34.953465487470524	36.0	36.0	36.0	36.0	36.0
33	34.924895355625836	36.0	36.0	36.0	36.0	36.0
34	34.910810398215936	36.0	36.0	36.0	36.0	36.0
35	34.88675921169624	36.0	36.0	36.0	36.0	36.0
36	34.87554178864003	36.0	36.0	36.0	32.0	36.0
37	34.83816585076031	36.0	36.0	36.0	32.0	36.0
38	34.81056222240101	36.0	36.0	36.0	32.0	36.0
39	34.76831766504311	36.0	36.0	36.0	32.0	36.0
40	34.770504417859584	36.0	36.0	36.0	32.0	36.0
41	34.73833336427795	36.0	36.0	36.0	32.0	36.0
42	34.71272566360727	36.0	36.0	36.0	32.0	36.0
43	34.706639889259534	36.0	36.0	36.0	32.0	36.0
44	34.634322323239196	36.0	36.0	36.0	32.0	36.0
45	34.627155550329356	36.0	36.0	36.0	32.0	36.0
46	34.56114862286147	36.0	36.0	36.0	32.0	36.0
47	34.541480225359315	36.0	36.0	36.0	32.0	36.0
48	34.50869027947114	36.0	36.0	36.0	32.0	36.0
49	34.48189946300778	36.0	36.0	36.0	32.0	36.0
50	34.093396006494245	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	9.0
21	25.0
22	80.0
23	183.0
24	448.0
25	1135.0
26	2417.0
27	4524.0
28	7726.0
29	12437.0
30	18110.0
31	27110.0
32	40244.0
33	68545.0
34	156261.0
35	630216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42095895315639	18.46576574996311	12.309203527587263	25.804071769293234
2	15.58260033566428	20.120566812561314	38.21693567603755	26.079897175736853
3	18.69285549247425	23.605942203591233	28.85859519408503	28.84260710984948
4	12.341640931061585	16.239940421300624	35.8414975543439	35.57692109329389
5	14.346233111976185	37.029873931637155	33.22564607199368	15.39824688439298
6	34.445998551792	35.499043811386386	16.626954410329724	13.428003226491892
7	30.141808857174095	30.388437441334165	20.411274567445854	19.058479134045886
8	27.592694595213484	34.43568368001617	19.11242591343347	18.859195811336868
9	27.1791713857205	14.068144168899838	18.959146918844652	39.793537526535005
10	16.764761097254798	27.047244175707654	30.82609745078259	25.361897276254957
11	37.333646905435316	20.91123640242028	22.528401999434745	19.226714692709653
12	24.680084251872664	23.516669864276917	29.24379612037043	22.559449763479993
13	29.768719945042367	19.646839420139166	25.13259767667828	25.451842958140187
14	23.165861075180974	20.371149716237877	25.067923430643834	31.395065777937315
15	24.807885513175183	28.56662478828726	21.91074747749811	24.71474222103945
16	25.01266157040607	25.897166386960286	24.92776973786681	24.162402304766836
17	23.40248423371849	26.534698197166712	25.655045932124015	24.40777163699078
18	24.16080522264204	25.274195927469723	27.474571183666143	23.090427666222094
19	25.73086023967636	24.720415400516156	25.378401071096285	24.1703232887112
20	25.69001334744408	24.894014692503358	25.25854226106115	24.15742969899141
21	26.470491153947716	24.283630677317134	24.6640439331925	24.581834235542647
22	25.76025762423747	25.102684548528376	25.288661686646574	23.848396140587578
23	24.04004645818248	24.913612948877432	26.046185869863454	25.00015472307664
24	24.83145325664491	25.057041829299486	26.10060817111311	24.010896742942492
25	24.63265973434942	24.79470720569714	26.141217511854425	24.43141554809901
26	23.696010281938428	26.164892667826756	26.610188897610747	23.52890815262407
27	24.533223857596273	25.23567024835245	25.90603722243826	24.325068671613018
28	23.853505319999382	25.510828248861472	26.003269878436853	24.63239655270229
29	24.08263930865033	24.980143707979444	25.782750503885644	25.154466479484583
30	23.76052259967136	25.314066269813846	26.39105647364876	24.534354656866032
31	25.089894849329426	24.79849731707971	25.423996303119452	24.687611530471415
32	24.548178634607748	25.306638197356275	25.33706735158051	24.808115816455466
33	23.976581042651095	25.02323432057277	26.221207235698646	24.778977401077494
34	24.722811034952265	25.380493364897134	26.358145987735504	23.538549612415093
35	25.013950907247356	25.16650833289495	26.253905480411483	23.565635279446216
36	23.520377113268072	25.71281215509505	26.008643898212426	24.75816683342445
37	24.61674619531245	25.518896047498508	25.418943702795982	24.445414054393055
38	24.29430804745666	25.419454260764084	25.559942814350872	24.72629487742838
39	25.32832440059909	24.568423093903046	25.33832988814484	24.764922617353022
40	25.804198978002507	25.006859389730927	25.694448742307685	23.494492889958885
41	24.322305934000642	25.05683552973602	26.65204690426875	23.968811631994587
42	25.309903395346677	25.975356271565843	25.730363157161797	22.984377175925687
43	24.458523693746756	25.07114983706971	26.053889232159417	24.416437237024116
44	23.81595370475434	25.859628854068884	25.502511785275882	24.821905655900892
45	24.13840766203692	26.066501281647938	25.453476437792368	24.341614618522776
46	24.0813690934841	25.222108413042736	25.53929562776765	25.15722686570551
47	24.910001258435123	25.16736155666362	25.74314720183855	24.1794899830627
48	25.12378382392989	25.680604725241174	24.641955289282798	24.55365616154614
49	24.62741376464763	25.979431424327448	24.89756973097871	24.49558508004621
50	24.648335518396305	26.51236099234523	24.483192206803576	24.35611128245489
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	484.0
1	267.5
2	51.0
3	164.0
4	277.0
5	280.0
6	283.0
7	212.5
8	142.0
9	206.0
10	270.0
11	427.5
12	585.0
13	1108.5
14	1632.0
15	2113.5
16	2595.0
17	2624.0
18	2653.0
19	2637.5
20	2622.0
21	3009.5
22	3397.0
23	3751.5
24	4106.0
25	5285.5
26	6465.0
27	8899.0
28	11333.0
29	13476.0
30	15619.0
31	17808.0
32	19997.0
33	23716.0
34	27435.0
35	31823.5
36	36212.0
37	39834.5
38	43457.0
39	44419.5
40	45382.0
41	46880.5
42	48379.0
43	49463.0
44	50547.0
45	54798.5
46	59050.0
47	62826.0
48	66602.0
49	69285.0
50	71968.0
51	69249.0
52	66530.0
53	65850.5
54	65171.0
55	66856.5
56	68542.0
57	66426.5
58	64311.0
59	58388.5
60	52466.0
61	45923.5
62	39381.0
63	33649.0
64	27917.0
65	23603.0
66	19289.0
67	16732.5
68	14176.0
69	12756.0
70	11336.0
71	8856.0
72	6376.0
73	5787.5
74	5199.0
75	3727.5
76	2256.0
77	2024.0
78	1792.0
79	1522.5
80	1253.0
81	1016.5
82	780.0
83	707.0
84	634.0
85	475.0
86	316.0
87	212.0
88	108.0
89	73.0
90	38.0
91	28.0
92	18.0
93	16.0
94	14.0
95	14.0
96	14.0
97	12.0
98	10.0
99	7.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032285548658344626
2	0.005466882041189346
3	2.0629743551657908E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.157435887914477E-4
17	0.0
18	3.0944615327486866E-4
19	0.0
20	0.0
21	2.0629743551657908E-4
22	0.0
23	0.0
24	0.0010314871775828954
25	5.157435887914477E-4
26	7.220410243080268E-4
27	5.157435887914477E-4
28	0.0019598256374075015
29	8.251897420663163E-4
30	0.001340933330857764
31	8.251897420663163E-4
32	9.28338459824606E-4
33	3.0944615327486866E-4
34	9.28338459824606E-4
35	3.0944615327486866E-4
36	4.1259487103315816E-4
37	0.0012377846130994746
38	0.0
39	6.188923065497373E-4
40	0.0
41	0.0010314871775828954
42	0.006085774347739084
43	0.004229097428089871
44	0.00453854358136474
45	0.0019598256374075015
46	0.0015472307663743435
47	0.0016503794841326326
48	0.004332246145848161
49	0.0035070564037818445
50	0.001340933330857764
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	969474.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.42405727237303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.66273341252266	41.178729366274254
2	14.821843462243637	16.13329714942591
3	4.581292534508842	7.479975818388125
4	1.8411055912827219	4.0080174457782825
5	0.8835523731841242	2.4043252480656943
6	0.5046117409981492	1.6477810975437082
7	0.31658494531112624	1.206088603662867
8	0.22340692890050834	0.9726969194820988
9	0.16604005486030124	0.8132916109702496
>10	0.8157657800574425	8.363128488510494
>50	0.09448912171179932	3.608782231253242
>100	0.08000237190162687	8.775802418368603
>500	0.007238309681173548	2.5861004879813927
>1k	0.0013333728360056538	0.8219831142950558
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1250	0.12893589719786194	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1223	0.1261508818183881	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1197	0.12346901515667258	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1098	0.11325729209860193	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1078	0.11119431774343613	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1056	0.10892504595275378	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1027	0.10593373313776337	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0314871775828955E-4
2	0.0	0.0	0.0	0.0	1.0314871775828955E-4
3	0.0	0.0	0.0	0.0	1.0314871775828955E-4
4	0.0	0.0	0.0	0.0	1.0314871775828955E-4
5	0.0	0.0	0.0	0.0	1.0314871775828955E-4
6	0.0	0.0	0.0	0.0	1.0314871775828955E-4
7	0.0	0.0	0.0	0.0	1.0314871775828955E-4
8	0.0	0.0	0.0	0.0	1.0314871775828955E-4
9	0.0	0.0	0.0	0.0	1.0314871775828955E-4
10	0.0	0.0	0.0	0.0	2.062974355165791E-4
11	0.0	0.0	0.0	0.0	2.062974355165791E-4
12	0.0	0.0	0.0	0.0	5.157435887914477E-4
13	0.0	0.0	0.0	0.0	6.188923065497373E-4
14	0.0	0.0	0.0	0.0	6.188923065497373E-4
15	0.0	0.0	0.0	0.0	7.220410243080268E-4
16	0.0	0.0	0.0	0.0	7.220410243080268E-4
17	0.0	0.0	0.0	1.0314871775828955E-4	7.220410243080268E-4
18	0.0	0.0	0.0	2.062974355165791E-4	7.220410243080268E-4
19	0.0	0.0	0.0	2.062974355165791E-4	7.220410243080268E-4
20	0.0	0.0	0.0	4.125948710331582E-4	7.220410243080268E-4
21	0.0	0.0	0.0	7.220410243080268E-4	7.220410243080268E-4
22	0.0	0.0	0.0	8.251897420663164E-4	7.220410243080268E-4
23	0.0	0.0	0.0	0.0014440820486160536	7.220410243080268E-4
24	0.0	0.0	0.0	0.0019598256374075015	7.220410243080268E-4
25	0.0	0.0	0.0	0.0025787179439572387	7.220410243080268E-4
26	0.0	0.0	0.0	0.0036102051215401343	7.220410243080268E-4
27	0.0	0.0	0.0	0.0046416922991230295	7.220410243080268E-4
28	0.0	0.0	0.0	0.006085774347739084	7.220410243080268E-4
29	0.0	0.0	0.0	0.008458194856179742	7.220410243080268E-4
30	0.0	0.0	0.0	0.014234523050643957	7.220410243080268E-4
31	0.0	0.0	0.0	0.02238327175354883	7.220410243080268E-4
32	0.0	0.0	0.0	0.03280129224713608	7.220410243080268E-4
33	0.0	0.0	0.0	0.039712256336941476	7.220410243080268E-4
34	0.0	0.0	0.0	0.0493050870884624	7.220410243080268E-4
35	0.0	0.0	0.0	0.061167189630665704	7.220410243080268E-4
36	0.0	0.0	0.0	0.07736153831871716	7.220410243080268E-4
37	0.0	0.0	0.0	0.09778498443485849	7.220410243080268E-4
38	0.0	0.0	0.0	0.12377846130994746	7.220410243080268E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTATCG	30	0.0057460056	29.331717	8
CGTACTC	30	0.0057460056	29.331717	3
GTATCAA	1610	0.0	25.154446	1
GTAGGAC	785	0.0	22.138906	3
GTATAGG	140	7.2759576E-12	22.01014	1
GGTTTCG	70	3.214593E-5	21.999922	24
GATATAC	475	0.0	20.851711	1
TGTAGGA	840	0.0	20.691471	2
CTGTAGG	840	0.0	20.437988	1
GTCCTAC	845	0.0	20.31705	1
GATTTCG	315	0.0	20.257032	41
TTAACGG	55	0.0044830735	19.99993	35
ATTTCGT	315	0.0	19.558514	42
TATACTG	125	2.2879249E-8	19.358934	5
TACTAGA	70	8.113483E-4	18.859995	42
TAGGACC	785	0.0	18.776035	4
GGTATCA	790	0.0	18.666828	1
CGTCGTA	275	0.0	18.398985	10
GGTATAG	60	0.007391397	18.341784	1
CCTACAG	960	0.0	18.332323	3
>>END_MODULE