FastQCFastQC Report
Thu 2 Feb 2017
SRR4062089_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062089_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361069
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT5470.15149458967676538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAA250.006018731719.0024959
AACGAAC250.006018731719.00249514
CGATAAC250.006018731719.00249510
TAGACAC402.7568178E-416.6225765
ATTCCGA350.002167970416.283346
GGTTAAT350.002169978816.2810821
CTGTAGG601.4635407E-615.828831
TTGGACT807.663402E-915.4352484
TAGGACT456.752008E-414.7756214
GGTATCA902.2737368E-914.7735741
CTCCTAG602.5643667E-514.2459471
CTAGGAC759.629421E-713.9313013
TCAAGAC551.9560134E-413.8161663
CTAAGGC500.00149730413.2980593
TGCACTG500.00149730413.2980595
GTATCAA3350.013.0410651
CTAGACA604.0835328E-412.6648184
TAGGAGG604.0835328E-412.6648184
TCCTAGA853.932155E-612.2906212
TTAATTC550.003063946712.0891463
Produced by FastQC (version 0.11.5)