##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062089_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	361069
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.352018589244715	32.0	32.0	32.0	32.0	32.0
2	30.988597747244988	32.0	32.0	32.0	32.0	32.0
3	31.003517333252095	32.0	32.0	32.0	32.0	32.0
4	30.98996867634718	32.0	32.0	32.0	32.0	32.0
5	30.94666393404031	32.0	32.0	32.0	32.0	32.0
6	34.6048677676566	36.0	36.0	36.0	32.0	36.0
7	34.562795476764826	36.0	36.0	36.0	32.0	36.0
8	34.51363312829404	36.0	36.0	36.0	32.0	36.0
9	34.63155518751264	36.0	36.0	36.0	32.0	36.0
10	34.35320672779995	36.0	36.0	36.0	32.0	36.0
11	34.61892048334252	36.0	36.0	36.0	32.0	36.0
12	34.469940648463314	36.0	36.0	36.0	32.0	36.0
13	34.53199250004847	36.0	36.0	36.0	32.0	36.0
14	34.45712315374624	36.0	36.0	36.0	32.0	36.0
15	34.4224261844689	36.0	36.0	36.0	32.0	36.0
16	34.414987163118404	36.0	36.0	36.0	32.0	36.0
17	34.37236096147828	36.0	36.0	36.0	32.0	36.0
18	34.35641110147922	36.0	36.0	36.0	32.0	36.0
19	34.36704341829401	36.0	36.0	36.0	32.0	36.0
20	34.3394171197195	36.0	36.0	36.0	32.0	36.0
21	34.330665329895396	36.0	36.0	36.0	32.0	36.0
22	34.294204154884525	36.0	36.0	36.0	32.0	36.0
23	34.270222589034226	36.0	36.0	36.0	32.0	36.0
24	34.24146631253306	36.0	36.0	36.0	32.0	36.0
25	33.908009272465925	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	4.0
5	26.0
6	71.0
7	18.0
8	62.0
9	67.0
10	22.0
11	7.0
12	17.0
13	14.0
14	51.0
15	55.0
16	81.0
17	150.0
18	207.0
19	260.0
20	405.0
21	606.0
22	843.0
23	1196.0
24	1767.0
25	2541.0
26	3511.0
27	4554.0
28	6078.0
29	8122.0
30	10436.0
31	14602.0
32	21075.0
33	31056.0
34	72549.0
35	180616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.267472615734015	16.810823535928435	11.74497666625123	26.176727182086328
2	16.954424097399936	18.915273577276302	37.569377513376985	26.56092481194678
3	19.645992699395677	21.660028914381304	28.19706092514942	30.496917461073597
4	13.417664019676545	15.534332856007246	34.681848775340065	36.366154348976146
5	15.374535259340524	35.34106839098611	33.52698681826493	15.757409531408435
6	35.17880104642398	34.387054937258895	16.494756795104866	13.939387221212254
7	31.58615603434652	29.737687737227493	19.557110655794517	19.119045572631467
8	28.22465043932492	33.925476963514825	18.37955787471546	19.470314722444805
9	27.34650983523827	15.000194098302472	18.095784739436894	39.55751132702237
10	17.247949148875986	26.40092960824445	30.86537874880055	25.485742494079016
11	36.70770570598889	21.44024007699327	21.95583981184354	19.8962144051743
12	24.88359129520804	23.10009068647163	28.420363688803963	23.595954329516367
13	29.894559936103654	19.58545026956271	24.351052736671623	26.168937057662017
14	23.289274118191823	19.20249770553804	24.956814205422972	32.551413970847165
15	25.243703911187776	26.620371012338424	21.915186878953687	26.220738197520117
16	25.645533396925398	25.9477805630116	23.386443798664565	25.02024224139843
17	24.546619193398108	25.83521895381339	24.80894227782954	24.80921957495896
18	24.603086580638724	25.58074408442347	25.617621125831118	24.198548209106686
19	25.99089021404947	25.2237941177286	24.369093263027743	24.416222405194183
20	25.771432531706978	24.963614942130434	24.95668445491718	24.308268071245408
21	26.081990663221745	24.602466151406617	24.30972155997383	25.00582162539781
22	26.036990931094618	24.341596360238768	24.64629605498457	24.975116653682043
23	25.17428629243507	24.718855074191215	24.869649098977984	25.237209534395728
24	25.01268293223849	24.652016378308996	24.958070087408274	25.377230602044236
25	25.167714535999025	24.715023895856202	24.95647738487298	25.16078418327179
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	16.0
2	62.5
3	109.0
4	109.0
5	109.0
6	218.0
7	327.0
8	327.0
9	327.0
10	385.5
11	444.0
12	444.0
13	444.0
14	573.5
15	703.0
16	703.0
17	703.0
18	1331.0
19	1959.0
20	1959.0
21	1959.0
22	3066.0
23	4173.0
24	4173.0
25	4173.0
26	6538.0
27	8903.0
28	8903.0
29	8903.0
30	11238.0
31	13573.0
32	13573.0
33	13573.0
34	17274.0
35	20975.0
36	20975.0
37	20975.0
38	25444.0
39	29913.0
40	29913.0
41	29913.0
42	33957.0
43	38001.0
44	38001.0
45	38001.0
46	42102.0
47	46203.0
48	46203.0
49	46203.0
50	47699.0
51	49195.0
52	49195.0
53	49195.0
54	47698.5
55	46202.0
56	46202.0
57	46202.0
58	41835.0
59	37468.0
60	37468.0
61	37468.0
62	32573.5
63	27679.0
64	27679.0
65	27679.0
66	22457.5
67	17236.0
68	17236.0
69	17236.0
70	13027.5
71	8819.0
72	8819.0
73	8819.0
74	6729.0
75	4639.0
76	4639.0
77	4639.0
78	3568.5
79	2498.0
80	2498.0
81	2498.0
82	1819.5
83	1141.0
84	1141.0
85	1141.0
86	822.0
87	503.0
88	503.0
89	503.0
90	353.5
91	204.0
92	204.0
93	204.0
94	128.0
95	52.0
96	52.0
97	52.0
98	93.0
99	134.0
100	134.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011078214967222332
2	2.769553741805583E-4
3	8.308661225416749E-4
4	0.008308661225416748
5	0.03184986803076421
6	0.06120713769390339
7	0.10856650667877885
8	0.11133606042058443
9	0.1182599447750984
10	0.13487726722593188
11	0.14346288382552919
12	0.1346003118517513
13	0.13432335647757077
14	0.11576734640747337
15	0.13515422260011242
16	0.12102949851690396
17	0.12324514151034845
18	0.11382865878820947
19	0.09915002395663985
20	0.09527264871811206
21	0.09554960409229261
22	0.1069047744336955
23	0.08641007674433418
24	0.09638047021483429
25	0.09333396109884814
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	361069.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.615053300268954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.18117439567962	28.519637054276558
2	17.392131506413698	16.214702743535234
3	8.297549002679412	11.60372067064483
4	4.463921648439413	8.323437822809108
5	2.672564978612977	6.229087946323803
6	1.6832376277904988	4.707852704188342
7	1.0548720664324245	3.442104232119862
8	0.7762703082055	2.8948705433932473
9	0.5440629636809251	2.282537164561975
>10	1.881109372556404	13.310954478588528
>50	0.035205186977970815	1.1454263536359672
>100	0.017304244446799146	1.1735196177732228
>500	5.966980843723843E-4	0.1521486681493133
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	547	0.15149458967676538	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATAA	25	0.0060187317	19.002495	9
AACGAAC	25	0.0060187317	19.002495	14
CGATAAC	25	0.0060187317	19.002495	10
TAGACAC	40	2.7568178E-4	16.622576	5
ATTCCGA	35	0.0021679704	16.28334	6
GGTTAAT	35	0.0021699788	16.281082	1
CTGTAGG	60	1.4635407E-6	15.82883	1
TTGGACT	80	7.663402E-9	15.435248	4
TAGGACT	45	6.752008E-4	14.775621	4
GGTATCA	90	2.2737368E-9	14.773574	1
CTCCTAG	60	2.5643667E-5	14.245947	1
CTAGGAC	75	9.629421E-7	13.931301	3
TCAAGAC	55	1.9560134E-4	13.816166	3
CTAAGGC	50	0.001497304	13.298059	3
TGCACTG	50	0.001497304	13.298059	5
GTATCAA	335	0.0	13.041065	1
CTAGACA	60	4.0835328E-4	12.664818	4
TAGGAGG	60	4.0835328E-4	12.664818	4
TCCTAGA	85	3.932155E-6	12.290621	2
TTAATTC	55	0.0030639467	12.089146	3
>>END_MODULE