FastQCFastQC Report
Thu 2 Feb 2017
SRR4062089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361069
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTTG353.2137838E-431.4279777
GTTTAAT300.005724750529.3490371
ATATAAG454.0469276E-529.3490351
GTATCAA3200.026.8268551
GGTATCA1550.025.5620631
TAGGACG651.8090905E-523.691864
ATACACC651.8090905E-523.691863
TCTAGGC752.0579373E-623.4662233
TATAAGG500.00257310722.0056782
AGTAGTG755.459815E-520.5329465
TATACTG755.459815E-520.5329465
CCCTATA550.004463778320.0107061
CCCCTAG550.00447811919.9996236
GCCGGCC808.967644E-519.2496368
TCTAGAT708.094197E-418.8620112
CTCGTAA600.0073794718.343151
GAACAAT600.007403098518.3329876
CTACAGT1456.2645995E-918.2065534
GTTGTGC851.4275228E-418.1173048
GGCAGTT1102.7859223E-617.9996597
Produced by FastQC (version 0.11.5)