##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062089_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	361069
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.242405191251535	32.0	32.0	32.0	32.0	32.0
2	31.560255795983593	32.0	32.0	32.0	32.0	32.0
3	31.628716394927284	32.0	32.0	32.0	32.0	32.0
4	31.66028930758387	32.0	32.0	32.0	32.0	32.0
5	31.668140992441888	32.0	32.0	32.0	32.0	32.0
6	35.3060938490981	36.0	36.0	36.0	36.0	36.0
7	35.301078187271685	36.0	36.0	36.0	36.0	36.0
8	35.254405667614776	36.0	36.0	36.0	36.0	36.0
9	35.36507426558359	36.0	36.0	36.0	36.0	36.0
10	35.252525140624094	36.0	36.0	36.0	36.0	36.0
11	35.35929420692444	36.0	36.0	36.0	36.0	36.0
12	35.302931018724955	36.0	36.0	36.0	36.0	36.0
13	35.31075777759929	36.0	36.0	36.0	36.0	36.0
14	35.25623634263811	36.0	36.0	36.0	36.0	36.0
15	35.238156695811604	36.0	36.0	36.0	36.0	36.0
16	35.28237816040701	36.0	36.0	36.0	36.0	36.0
17	35.270873434163555	36.0	36.0	36.0	36.0	36.0
18	35.26230443488641	36.0	36.0	36.0	36.0	36.0
19	35.25267746607989	36.0	36.0	36.0	36.0	36.0
20	35.25227865034107	36.0	36.0	36.0	36.0	36.0
21	35.23892940130557	36.0	36.0	36.0	36.0	36.0
22	35.21553498084854	36.0	36.0	36.0	36.0	36.0
23	35.17173725797562	36.0	36.0	36.0	36.0	36.0
24	35.1907557835206	36.0	36.0	36.0	36.0	36.0
25	35.1454486538584	36.0	36.0	36.0	36.0	36.0
26	35.127125286302615	36.0	36.0	36.0	36.0	36.0
27	35.11221954806422	36.0	36.0	36.0	36.0	36.0
28	35.083507584422925	36.0	36.0	36.0	36.0	36.0
29	35.04863890281359	36.0	36.0	36.0	36.0	36.0
30	35.06305719959343	36.0	36.0	36.0	36.0	36.0
31	35.02973669852577	36.0	36.0	36.0	36.0	36.0
32	34.98203944398439	36.0	36.0	36.0	36.0	36.0
33	34.96309569639044	36.0	36.0	36.0	36.0	36.0
34	34.937842905372655	36.0	36.0	36.0	36.0	36.0
35	34.9262190883183	36.0	36.0	36.0	36.0	36.0
36	34.913188337963106	36.0	36.0	36.0	32.0	36.0
37	34.8958703184156	36.0	36.0	36.0	32.0	36.0
38	34.89806657453285	36.0	36.0	36.0	32.0	36.0
39	34.87419856038596	36.0	36.0	36.0	32.0	36.0
40	34.894036873838516	36.0	36.0	36.0	36.0	36.0
41	34.8776854285469	36.0	36.0	36.0	36.0	36.0
42	34.85147714148819	36.0	36.0	36.0	32.0	36.0
43	34.84325433642877	36.0	36.0	36.0	32.0	36.0
44	34.817486408415014	36.0	36.0	36.0	32.0	36.0
45	34.80060597835871	36.0	36.0	36.0	32.0	36.0
46	34.78040762291972	36.0	36.0	36.0	32.0	36.0
47	34.769839005840986	36.0	36.0	36.0	32.0	36.0
48	34.73361601245191	36.0	36.0	36.0	32.0	36.0
49	34.71647801389762	36.0	36.0	36.0	32.0	36.0
50	34.34170477111023	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	9.0
22	29.0
23	71.0
24	189.0
25	425.0
26	950.0
27	1708.0
28	2791.0
29	4350.0
30	6245.0
31	9287.0
32	13952.0
33	22577.0
34	52114.0
35	246369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.89210093997512	17.30878445952123	12.027138214850664	26.771976385652984
2	16.241472811320286	19.388950591467687	37.13685878172336	27.232717815488673
3	18.87118528591488	22.206281901797166	28.49289194032166	30.429640871966303
4	13.073160734266121	15.960151550400479	34.673247144582184	36.293440570751216
5	14.955867161124328	35.87846090359461	33.44651576291512	15.719156172365947
6	35.369237927481805	34.42066314378455	16.420729613258445	13.7893693154752
7	31.51973722474098	29.812030387543654	19.49599661006622	19.172235777649146
8	28.29237624941493	33.91180079153846	18.40423852504646	19.39158443400015
9	27.395594747818286	14.731256352663896	18.034226145141233	39.83892275437659
10	17.15350805524706	26.62538185222215	30.729029631455486	25.492080461075307
11	36.723174794845306	21.304792158839447	22.10076190423437	19.871271142080875
12	24.856190921956745	23.2371098044972	28.44082432997571	23.46587494357034
13	29.809814744550213	19.493504011698594	24.53049140192041	26.166189841830782
14	23.308841246409965	19.295757874533674	24.975835643602746	32.41956523545361
15	25.304304717380887	26.687419856038595	21.745151203786534	26.263124222793984
16	25.692738466821947	25.79299686761737	23.475975372991716	25.038289292568972
17	24.65401349880494	25.736632056476736	24.822956277055077	24.786398167663247
18	24.76070989398119	25.63450651954756	25.52095450164512	24.083829084826128
19	26.002232260315893	25.187429549476693	24.62105580927745	24.18928238092996
20	25.90142050411417	24.83818882263501	24.89884204958055	24.361548623670267
21	26.311314457901396	24.567326466686424	24.31917445142064	24.802184623991536
22	26.095566221414746	24.240519125153366	24.746239638407065	24.91767501502483
23	25.10018860660982	24.751501790516496	24.860622207943635	25.287687394930053
24	25.083363798508866	24.63275615673502	24.91137403480785	25.372506009948264
25	25.26303836662799	24.54544699212616	24.939277534210913	25.25223710703494
26	25.038773859771567	25.221287956839156	25.236520544606556	24.503417638782725
27	25.450053729491394	24.569887112676835	25.06231513177573	24.917744026056035
28	25.410040380876413	24.650060100481358	24.83008458381109	25.10981493483114
29	25.088211019591988	25.05248347947467	24.880215805420615	24.979089695512734
30	25.08827952552005	24.800799847120633	25.117082884434833	24.993837742924484
31	25.60791872998992	24.48569244574429	24.786743771256383	25.119645053009403
32	25.226343033287453	25.030534499137282	24.684892277610526	25.058230189964743
33	24.94204708795272	24.898565094206372	24.945924463191247	25.21346335464967
34	25.292188728992876	24.906942736548242	24.767079885229375	25.03378864922951
35	25.616156468708194	24.77365822045094	25.084679105655706	24.525506205185156
36	25.16527311954225	24.699988090918907	24.878901262639552	25.25583752689929
37	25.382133410881696	24.79885893121737	24.805782892277016	25.01322476562392
38	25.34363238051453	24.894410763593662	24.98912950156341	24.7728273543284
39	25.724034597456978	24.817554636674082	24.512902037571973	24.945508728296964
40	25.5377780978151	24.877793441142828	24.896349451212927	24.68807900982915
41	25.211040579613815	24.821086332768346	25.052344710691614	24.91552837692623
42	25.371823917486942	25.02035684017526	24.810141306936835	24.797677935400962
43	25.42590228135724	24.60387141088526	24.95007713463525	25.020149173122245
44	25.24005638936357	24.930690381957618	24.87419023378321	24.9550629948956
45	25.2627672332376	24.898839808231852	24.6864103295565	25.15198262897405
46	25.304588421347084	24.49918434835111	24.880283387017705	25.3159438432841
47	25.093126760407348	24.641963956827954	25.2094486618827	25.055460620882
48	25.402144772117964	24.880907539937517	24.5144905058383	25.20245718210622
49	25.270318177789598	24.97562704954356	24.5815053620491	25.172549410617744
50	25.12774637394168	25.330478830798715	24.443662810503312	25.098111984756294
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	4.0
2	7.0
3	17.0
4	27.0
5	26.5
6	26.0
7	25.5
8	25.0
9	35.5
10	46.0
11	73.0
12	100.0
13	211.5
14	323.0
15	422.5
16	522.0
17	540.5
18	559.0
19	551.0
20	543.0
21	596.0
22	649.0
23	870.5
24	1092.0
25	1357.0
26	1622.0
27	2227.5
28	2833.0
29	3440.0
30	4047.0
31	4925.5
32	5804.0
33	6535.0
34	7266.0
35	8848.5
36	10431.0
37	12094.0
38	13757.0
39	14717.0
40	15677.0
41	16842.0
42	18007.0
43	18889.5
44	19772.0
45	21498.0
46	23224.0
47	23520.0
48	23816.0
49	26110.5
50	28405.0
51	28317.0
52	28229.0
53	28334.5
54	28440.0
55	28062.0
56	27684.0
57	26262.0
58	24840.0
59	23028.0
60	21216.0
61	18400.5
62	15585.0
63	13505.5
64	11426.0
65	9521.5
66	7617.0
67	6380.5
68	5144.0
69	4421.0
70	3698.0
71	3080.5
72	2463.0
73	2231.0
74	1999.0
75	1650.5
76	1302.0
77	1158.0
78	1014.0
79	914.0
80	814.0
81	638.0
82	462.0
83	356.0
84	250.0
85	217.0
86	184.0
87	122.0
88	60.0
89	45.5
90	31.0
91	19.0
92	7.0
93	9.0
94	11.0
95	8.0
96	5.0
97	4.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028249448166416943
2	0.004985196735250049
3	0.0
4	2.769553741805583E-4
5	0.0
6	2.769553741805583E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.539107483611166E-4
17	0.0
18	2.769553741805583E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	2.769553741805583E-4
25	0.0
26	2.769553741805583E-4
27	2.769553741805583E-4
28	0.001938687619263908
29	8.308661225416749E-4
30	0.0
31	2.769553741805583E-4
32	5.539107483611166E-4
33	0.0
34	2.769553741805583E-4
35	0.0
36	0.0
37	0.0011078214967222332
38	0.0
39	5.539107483611166E-4
40	0.0
41	2.769553741805583E-4
42	0.003046509115986141
43	0.0033234644901666997
44	0.0022156429934444663
45	0.0022156429934444663
46	0.0022156429934444663
47	5.539107483611166E-4
48	0.0013847768709027914
49	0.003600419864347258
50	5.539107483611166E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	361069.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.170602977062394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.10921691147864	28.825586091705457
2	17.535586649214785	16.54328391599973
3	8.184401694724505	11.58189488839939
4	4.414126778334051	8.3286808700486
5	2.703364983563337	6.3759678170879495
6	1.6344840631918025	4.6259759290293765
7	1.1694598876874283	3.8614889641792556
8	0.7815377179392213	2.94924843236083
9	0.5686385780322071	2.414072214161869
>10	1.859641062714925	12.999131727357613
>50	0.03127923395993412	1.0066536869447775
>100	0.008262439159227827	0.48801546272516216
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	2.769553741805583E-4	0.0
14	0.0	0.0	0.0	2.769553741805583E-4	0.0
15	0.0	0.0	0.0	2.769553741805583E-4	0.0
16	0.0	0.0	0.0	5.539107483611166E-4	0.0
17	0.0	0.0	0.0	8.308661225416748E-4	0.0
18	0.0	0.0	0.0	8.308661225416748E-4	0.0
19	0.0	0.0	0.0	8.308661225416748E-4	0.0
20	0.0	0.0	0.0	0.0013847768709027914	0.0
21	0.0	0.0	0.0	0.001938687619263908	0.0
22	0.0	0.0	0.0	0.0024925983676250246	0.0
23	0.0	0.0	0.0	0.0033234644901666993	0.0
24	0.0	0.0	0.0	0.004154330612708374	0.0
25	0.0	0.0	0.0	0.005539107483611166	0.0
26	0.0	0.0	0.0	0.0066469289803333985	0.0
27	0.0	0.0	0.0	0.007200839728694516	0.0
28	0.0	0.0	0.0	0.009416482722138981	0.0
29	0.0	0.0	0.0	0.01135517034140289	0.0
30	0.0	0.0	0.0	0.015509500954111265	0.0
31	0.0	0.0	0.0	0.024372072927889128	0.0
32	0.0	0.0	0.0	0.03268073415330588	0.0
33	0.0	0.0	0.0	0.04015852925618095	0.0
34	0.0	0.0	0.0	0.04708241361069491	0.0
35	0.0	0.0	0.0	0.05982236082300059	0.0
36	0.0	0.0	0.0	0.0736701295320285	0.0
37	0.0	0.0	0.0	0.09804220245991763	0.0
38	0.0	0.0	0.0	0.13183075810994574	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTTG	35	3.2137838E-4	31.427977	7
GTTTAAT	30	0.0057247505	29.349037	1
ATATAAG	45	4.0469276E-5	29.349035	1
GTATCAA	320	0.0	26.826855	1
GGTATCA	155	0.0	25.562063	1
TAGGACG	65	1.8090905E-5	23.69186	4
ATACACC	65	1.8090905E-5	23.69186	3
TCTAGGC	75	2.0579373E-6	23.466223	3
TATAAGG	50	0.002573107	22.005678	2
AGTAGTG	75	5.459815E-5	20.532946	5
TATACTG	75	5.459815E-5	20.532946	5
CCCTATA	55	0.0044637783	20.010706	1
CCCCTAG	55	0.004478119	19.99962	36
GCCGGCC	80	8.967644E-5	19.249636	8
TCTAGAT	70	8.094197E-4	18.862011	2
CTCGTAA	60	0.00737947	18.34315	1
GAACAAT	60	0.0074030985	18.332987	6
CTACAGT	145	6.2645995E-9	18.206553	4
GTTGTGC	85	1.4275228E-4	18.117304	8
GGCAGTT	110	2.7859223E-6	17.999659	7
>>END_MODULE