FastQCFastQC Report
Thu 2 Feb 2017
SRR4062088_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062088_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344610
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33090.9602158962305214No Hit
TATCAACGCAGAGTACTTTTTTTTT19770.5736919996517802No Hit
GGTATCAACGCAGAGTACTTTTTTT12660.3673718116131279No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10060.29192420417283305No Hit
CCATAGGGTCTTCTCGTCTTATTAT9360.2716113867850614No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6660.1932619482893706No Hit
GTATCAACGCAGAGTACATGGGAGA5830.16917675052958417No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5700.16540437015756942No Hit
GTCAGGATACCGCGGCCGTTAAACT5390.15640869388584197No Hit
GTATCAACGCAGAGTACATGGGGTG5340.15495777835814398No Hit
CCATTGGGATGTCCTGATCCAACAT5080.1474130176141145No Hit
GTATTAGAGGCACTGCCTGCCCAGT4610.1337744116537535No Hit
CTTCTACACCATTGGGATGTCCTGA4580.13290386233713472No Hit
GTACATGGGGTGGTATCAACGCAAA4520.13116276370389715No Hit
GGTATCAACGCAGAGTACATGGGAG4420.12826093264850122No Hit
GTACATGGGAGAAATCGTAAATAGA4260.12361800295986768No Hit
GGTATCAACGCAGAGTACATGGGGG4230.12274745364324888No Hit
ACCTATAACTTCTCTGTTAACCCAA4160.12071617190447172No Hit
CCTATAACTTCTCTGTTAACCCAAC4120.11955543948231333No Hit
GTTCATGCTAGTCCCTAATTAAGGA4070.11810452395461536No Hit
ATAAATAATCCACCTATAACTTCTC4000.11607324221583819No Hit
GCCTAAAGGAAAGATCCAAAAAGAT3990.11578305911029858No Hit
GGTCAGGATACCGCGGCCGTTAAAC3960.11491250979367981No Hit
CTGTTAACCCAACACCGGAATGCCT3820.11084994631612548No Hit
GGTATCAACGCAGAGTACATGGGGT3810.11055976321058589No Hit
GACTATAGGCAATAATCACACTATA3770.1093990307884275No Hit
ATCCTGACCGTGCAAAGGTAGCATA3730.10823829836626912No Hit
GATATATTTTGATCAACGGACCAAG3600.10446591799425438No Hit
ATCGTAAATAGATAGAAACCGACCT3550.1030150024665564No Hit
CCTCTAGCATTACAAGTATTAGAGG3500.10156408693885843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAT250.006025940618.9982571
TCCTAAG456.7406823E-414.7785682
GTCCTTG400.005266185414.25076411
GTAGGCA400.005266185414.2507643
AGTGCTT400.005266185414.2507648
GGTAGGC400.005266185414.2507642
GAATTTT400.005271266714.24869217
CTTGGAC500.001494809413.3007123
TGGCACA500.001494809413.3007124
TAGGACC500.001494809413.3007124
TCTTAGG655.4257354E-513.154552
CTAGGAC604.0758128E-412.6673453
CTGTAGG1207.3123374E-1012.6655041
TAGGGTC1301.9463187E-1012.4237414
GTATCAA20300.012.1663711
TGTAGGA1251.3915269E-912.1606512
TAACTTA550.003055549712.0933136
GGGTAGG550.003062211712.0897991
GAGGTCG550.003062211712.08979917
TCGAGGT550.003062211712.08979915
Produced by FastQC (version 0.11.5)