##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062088_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	344610
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.157932155189926	32.0	32.0	32.0	32.0	32.0
2	30.907182031862106	32.0	32.0	32.0	32.0	32.0
3	30.941893734946753	32.0	32.0	32.0	32.0	32.0
4	30.91482545486202	32.0	32.0	32.0	32.0	32.0
5	30.95562229766983	32.0	32.0	32.0	32.0	32.0
6	34.561750964858824	36.0	36.0	36.0	32.0	36.0
7	34.410844142654014	36.0	36.0	36.0	32.0	36.0
8	34.38121934940948	36.0	36.0	36.0	32.0	36.0
9	34.41129682829866	36.0	36.0	36.0	32.0	36.0
10	34.2969356664055	36.0	36.0	36.0	32.0	36.0
11	34.50843852470909	36.0	36.0	36.0	32.0	36.0
12	34.38591161022605	36.0	36.0	36.0	32.0	36.0
13	34.43018484663823	36.0	36.0	36.0	32.0	36.0
14	34.35884913380343	36.0	36.0	36.0	32.0	36.0
15	34.36954818490467	36.0	36.0	36.0	32.0	36.0
16	34.354241026087465	36.0	36.0	36.0	32.0	36.0
17	34.278903688227274	36.0	36.0	36.0	32.0	36.0
18	34.319552537651255	36.0	36.0	36.0	32.0	36.0
19	34.261121267519805	36.0	36.0	36.0	32.0	36.0
20	34.197533443602914	36.0	36.0	36.0	32.0	36.0
21	34.16516932184208	36.0	36.0	36.0	32.0	36.0
22	34.17265024230289	36.0	36.0	36.0	32.0	36.0
23	34.16956849772206	36.0	36.0	36.0	32.0	36.0
24	34.16537535184702	36.0	36.0	36.0	32.0	36.0
25	33.85970227213372	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	7.0
5	35.0
6	84.0
7	17.0
8	51.0
9	55.0
10	25.0
11	8.0
12	13.0
13	21.0
14	102.0
15	124.0
16	185.0
17	231.0
18	313.0
19	440.0
20	645.0
21	823.0
22	1132.0
23	1585.0
24	1982.0
25	2775.0
26	3547.0
27	4406.0
28	5792.0
29	7637.0
30	9984.0
31	13739.0
32	19777.0
33	28931.0
34	68831.0
35	171312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.65998850861564	20.128204384137245	13.078124002483996	22.133683104763122
2	15.068381847252974	21.134967455870278	40.91390532458525	22.882745372291495
3	18.056458352678987	27.55246540997307	29.35161110595227	25.039465131395673
4	10.420965565277958	17.4570835691379	38.8518857109689	33.27006515461524
5	11.79477800421522	40.74799835107093	33.08512306004076	14.37210058467309
6	29.24733493435706	38.88832423924754	18.974454282254484	12.889886544140921
7	28.134306026455626	34.989061560318305	19.06280959561184	17.813822817614227
8	27.94296239911213	32.58075387717392	18.869300452651704	20.606983271062248
9	27.83035452255247	15.417851943757574	20.818938088582694	35.932855445107265
10	15.836065192625142	28.72637232335914	31.142905303294505	24.29465718072121
11	33.331395805057525	25.117632185444705	22.337182233253216	19.213789776244546
12	24.72718607145037	28.403163043193963	27.908828563872607	18.96082232148306
13	27.115885889638296	23.257091214478546	26.496630565233943	23.130392330649215
14	22.69437398487371	22.79170988671263	28.2477503784478	26.266165749965857
15	24.253184861446904	28.814278408034223	25.659928863678633	21.272607866840247
16	22.903549169729637	29.735156102450862	25.502012116281435	21.85928261153807
17	20.157318324068775	28.75112961762988	26.676701255001557	24.414850803299792
18	20.07083173540657	28.539843577496544	29.33473950888447	22.054585178212413
19	22.693496473431633	27.208607847921822	30.179465727797727	19.918429950848818
20	24.646786805628174	26.44306180069016	28.91206850476954	19.998082888912126
21	26.407908573818972	25.231092803151345	27.611450350633582	20.749548272396105
22	24.079157987699833	27.38021573224334	27.70587671364203	20.834749566414796
23	22.856221698757142	27.31993946910572	28.199438259359198	21.624400572777944
24	21.6171703440343	27.78387767669374	28.807498809067354	21.79145317020461
25	21.78065400635533	27.823735469591437	29.330366738507834	21.065243785545402
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	250.0
1	250.0
2	296.5
3	343.0
4	343.0
5	343.0
6	860.5
7	1378.0
8	1378.0
9	1378.0
10	1407.0
11	1436.0
12	1436.0
13	1436.0
14	1699.0
15	1962.0
16	1962.0
17	1962.0
18	3167.0
19	4372.0
20	4372.0
21	4372.0
22	7002.0
23	9632.0
24	9632.0
25	9632.0
26	14102.5
27	18573.0
28	18573.0
29	18573.0
30	22041.5
31	25510.0
32	25510.0
33	25510.0
34	27999.0
35	30488.0
36	30488.0
37	30488.0
38	32099.0
39	33710.0
40	33710.0
41	33710.0
42	35661.5
43	37613.0
44	37613.0
45	37613.0
46	42852.5
47	48092.0
48	48092.0
49	48092.0
50	47798.5
51	47505.0
52	47505.0
53	47505.0
54	41188.5
55	34872.0
56	34872.0
57	34872.0
58	28188.0
59	21504.0
60	21504.0
61	21504.0
62	17011.0
63	12518.0
64	12518.0
65	12518.0
66	9885.5
67	7253.0
68	7253.0
69	7253.0
70	5505.5
71	3758.0
72	3758.0
73	3758.0
74	2945.5
75	2133.0
76	2133.0
77	2133.0
78	1571.5
79	1010.0
80	1010.0
81	1010.0
82	686.0
83	362.0
84	362.0
85	362.0
86	250.5
87	139.0
88	139.0
89	139.0
90	88.5
91	38.0
92	38.0
93	38.0
94	32.5
95	27.0
96	27.0
97	27.0
98	79.5
99	132.0
100	132.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011607324221583819
2	2.9018310553959546E-4
3	5.803662110791909E-4
4	0.013058239749281797
5	0.04178636719770175
6	0.07167522706828008
7	0.11926525637677374
8	0.12071617190447172
9	0.13261367923159514
10	0.15147558109166884
11	0.153506862830446
12	0.1482835669307333
13	0.141319172397783
14	0.12797074954296161
15	0.139868256870085
16	0.12913148196512
17	0.1355155102869911
18	0.12013580569339252
19	0.10620701662749195
20	0.09924262209454165
21	0.1091088476828879
22	0.11230086184382344
23	0.09372914308928934
24	0.09924262209454165
25	0.0963407910391457
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	344610.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.380191445517795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.46680095757966	38.020318800236495
2	14.215650743755576	14.323744119860468
3	4.735813656407774	7.157735959803638
4	1.9976326908649045	4.02564469614395
5	0.9795956475190656	2.4676108130603245
6	0.5646512320823103	1.7068342303352575
7	0.3714667384445439	1.3100195778934747
8	0.25457406830609985	1.0260392238660507
9	0.16725215245516964	0.7583575914329738
>10	0.9930158868990319	10.138863964075135
>50	0.13976807885187417	4.889798466849204
>100	0.10742505234069667	10.714201674877796
>500	0.004042878313897187	1.2616582060254773
>1k	0.002310216179369821	2.1991726755397965
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3309	0.9602158962305214	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1977	0.5736919996517802	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1266	0.3673718116131279	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1006	0.29192420417283305	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	936	0.2716113867850614	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	666	0.1932619482893706	No Hit
GTATCAACGCAGAGTACATGGGAGA	583	0.16917675052958417	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	570	0.16540437015756942	No Hit
GTCAGGATACCGCGGCCGTTAAACT	539	0.15640869388584197	No Hit
GTATCAACGCAGAGTACATGGGGTG	534	0.15495777835814398	No Hit
CCATTGGGATGTCCTGATCCAACAT	508	0.1474130176141145	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	461	0.1337744116537535	No Hit
CTTCTACACCATTGGGATGTCCTGA	458	0.13290386233713472	No Hit
GTACATGGGGTGGTATCAACGCAAA	452	0.13116276370389715	No Hit
GGTATCAACGCAGAGTACATGGGAG	442	0.12826093264850122	No Hit
GTACATGGGAGAAATCGTAAATAGA	426	0.12361800295986768	No Hit
GGTATCAACGCAGAGTACATGGGGG	423	0.12274745364324888	No Hit
ACCTATAACTTCTCTGTTAACCCAA	416	0.12071617190447172	No Hit
CCTATAACTTCTCTGTTAACCCAAC	412	0.11955543948231333	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	407	0.11810452395461536	No Hit
ATAAATAATCCACCTATAACTTCTC	400	0.11607324221583819	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	399	0.11578305911029858	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	396	0.11491250979367981	No Hit
CTGTTAACCCAACACCGGAATGCCT	382	0.11084994631612548	No Hit
GGTATCAACGCAGAGTACATGGGGT	381	0.11055976321058589	No Hit
GACTATAGGCAATAATCACACTATA	377	0.1093990307884275	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	373	0.10823829836626912	No Hit
GATATATTTTGATCAACGGACCAAG	360	0.10446591799425438	No Hit
ATCGTAAATAGATAGAAACCGACCT	355	0.1030150024665564	No Hit
CCTCTAGCATTACAAGTATTAGAGG	350	0.10156408693885843	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAT	25	0.0060259406	18.998257	1
TCCTAAG	45	6.7406823E-4	14.778568	2
GTCCTTG	40	0.0052661854	14.250764	11
GTAGGCA	40	0.0052661854	14.250764	3
AGTGCTT	40	0.0052661854	14.250764	8
GGTAGGC	40	0.0052661854	14.250764	2
GAATTTT	40	0.0052712667	14.248692	17
CTTGGAC	50	0.0014948094	13.300712	3
TGGCACA	50	0.0014948094	13.300712	4
TAGGACC	50	0.0014948094	13.300712	4
TCTTAGG	65	5.4257354E-5	13.15455	2
CTAGGAC	60	4.0758128E-4	12.667345	3
CTGTAGG	120	7.3123374E-10	12.665504	1
TAGGGTC	130	1.9463187E-10	12.423741	4
GTATCAA	2030	0.0	12.166371	1
TGTAGGA	125	1.3915269E-9	12.160651	2
TAACTTA	55	0.0030555497	12.093313	6
GGGTAGG	55	0.0030622117	12.089799	1
GAGGTCG	55	0.0030622117	12.089799	17
TCGAGGT	55	0.0030622117	12.089799	15
>>END_MODULE