FastQCFastQC Report
Thu 2 Feb 2017
SRR4062088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344610
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.2710310205739822No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG8800.255361132874844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.16917675052958417No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC5180.15031484866951045No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT4860.1410289892922434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.12942166507065958No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA4370.12681001712080323No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC4310.12506891848756566No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA4230.12274745364324888No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA4120.11955543948231333No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC4080.11839470706015497No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC4050.11752415774353617No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC4010.11636342532137778No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT3860.11201067873828387No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG3790.10997939699950668No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT3730.10823829836626912No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA3690.10707756594411073No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC3670.10649719973303154No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA3620.10504628420533357No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG3590.10417573488871479No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC3520.1021444531499376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC554.9516148E-627.9970463
GTTTGTC450.001397047124.4418649
GCGTGAA450.001397047124.44186418
TAGGACG901.5063051E-824.4418644
GTATCAA19200.023.7196881
GGTATCA9250.023.309031
GTTACTC703.205779E-522.0008735
AAAGCGT803.6064375E-621.99767910
TGTAGGA1650.021.3310812
ACGATTA755.4564298E-520.53414740
CGTATGC755.4625314E-520.5311669
GCGTATG755.4625314E-520.5311668
GTTATAT1101.2585406E-720.0007921
ATAGCCC1101.2604687E-719.997893
GTATAGC1553.274181E-1119.8717561
TCCTCGC909.982157E-619.5563344
TTACTCA909.982157E-619.5563336
AGCGTTC909.994677E-619.55349212
GAAAGCG909.994677E-619.5534929
GACGTGG808.972102E-519.2479697
Produced by FastQC (version 0.11.5)