##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062088_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	344610
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.158071443080583	32.0	32.0	32.0	32.0	32.0
2	31.495226487913875	32.0	32.0	32.0	32.0	32.0
3	31.612222512405328	32.0	32.0	32.0	32.0	32.0
4	31.69113200429471	32.0	32.0	32.0	32.0	32.0
5	31.67305649865065	32.0	32.0	32.0	32.0	32.0
6	35.3111314239285	36.0	36.0	36.0	36.0	36.0
7	35.309332288674156	36.0	36.0	36.0	36.0	36.0
8	35.28734801659847	36.0	36.0	36.0	36.0	36.0
9	35.296718029076345	36.0	36.0	36.0	36.0	36.0
10	35.24357969878994	36.0	36.0	36.0	36.0	36.0
11	35.34395113316503	36.0	36.0	36.0	36.0	36.0
12	35.281355735469084	36.0	36.0	36.0	36.0	36.0
13	35.30961956994864	36.0	36.0	36.0	36.0	36.0
14	35.2652070456458	36.0	36.0	36.0	36.0	36.0
15	35.268558660514785	36.0	36.0	36.0	36.0	36.0
16	35.26044223905284	36.0	36.0	36.0	36.0	36.0
17	35.2537245001596	36.0	36.0	36.0	36.0	36.0
18	35.24353907315516	36.0	36.0	36.0	36.0	36.0
19	35.21878935608369	36.0	36.0	36.0	36.0	36.0
20	35.18841008676475	36.0	36.0	36.0	36.0	36.0
21	35.165674240445725	36.0	36.0	36.0	36.0	36.0
22	35.17460317460318	36.0	36.0	36.0	36.0	36.0
23	35.149026435680916	36.0	36.0	36.0	36.0	36.0
24	35.149513943298224	36.0	36.0	36.0	36.0	36.0
25	35.146365456603114	36.0	36.0	36.0	36.0	36.0
26	35.11793041409129	36.0	36.0	36.0	36.0	36.0
27	35.07765009721134	36.0	36.0	36.0	36.0	36.0
28	35.08274281071356	36.0	36.0	36.0	36.0	36.0
29	35.06115028583036	36.0	36.0	36.0	36.0	36.0
30	35.01918110327617	36.0	36.0	36.0	36.0	36.0
31	35.02147935347204	36.0	36.0	36.0	36.0	36.0
32	35.00351121557703	36.0	36.0	36.0	36.0	36.0
33	34.93846086880822	36.0	36.0	36.0	36.0	36.0
34	34.92432604973738	36.0	36.0	36.0	36.0	36.0
35	34.899167174487104	36.0	36.0	36.0	36.0	36.0
36	34.85646673050695	36.0	36.0	36.0	36.0	36.0
37	34.85127535474884	36.0	36.0	36.0	36.0	36.0
38	34.81841211804649	36.0	36.0	36.0	32.0	36.0
39	34.76312643277908	36.0	36.0	36.0	32.0	36.0
40	34.75094454600853	36.0	36.0	36.0	32.0	36.0
41	34.687289979977365	36.0	36.0	36.0	32.0	36.0
42	34.662255883462464	36.0	36.0	36.0	32.0	36.0
43	34.6029018310554	36.0	36.0	36.0	32.0	36.0
44	34.51665070659586	36.0	36.0	36.0	32.0	36.0
45	34.441557122544324	36.0	36.0	36.0	32.0	36.0
46	34.33317083079423	36.0	36.0	36.0	32.0	36.0
47	34.251545225037	36.0	36.0	36.0	32.0	36.0
48	34.18867125155973	36.0	36.0	36.0	32.0	36.0
49	34.113949101883286	36.0	36.0	36.0	32.0	36.0
50	33.70318621049883	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	2.0
21	16.0
22	42.0
23	115.0
24	257.0
25	433.0
26	1040.0
27	1918.0
28	3243.0
29	5005.0
30	7419.0
31	10371.0
32	15038.0
33	23490.0
34	48593.0
35	227626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17221920655332	20.81817838762021	13.596344815774883	22.413257590051582
2	14.755045067528744	21.618194164727186	40.46472080187119	23.162039965872882
3	17.662575084878558	27.66895911320043	29.7588578392966	24.909607962624417
4	10.385943530367662	17.7896752851049	38.742346420591396	33.08203476393604
5	11.323525144366094	40.99590841821189	33.156321638954175	14.524244798467834
6	29.356459059397753	39.000432374081925	18.715703884692505	12.927404681827811
7	27.916775485331247	34.99114941528104	19.31894025129857	17.773134848089146
8	27.946083978990742	32.83595949043846	18.67299265836743	20.544963872203358
9	27.724384086358494	15.188764110153507	20.907982937233392	36.178868866254604
10	15.80366211079191	28.775717477728445	31.09660195583413	24.324018455645515
11	33.4305446736891	24.87101360958765	22.495864890746063	19.20257682597719
12	24.667885435709934	28.49046748498302	27.967557528800675	18.87408955050637
13	27.277502103827516	23.031252720466615	26.676242709149474	23.015002466556396
14	22.837410405966168	22.729752473810976	28.20028437944343	26.23255274077943
15	24.42529235947883	28.655291488929514	25.763036476016367	21.156379675575288
16	23.00076028856143	29.316378704955802	25.701235614005558	21.981625392477206
17	20.276544499579234	28.354371608484954	26.816691332230636	24.552392559705176
18	20.138997707553465	28.07086271437277	29.782072487739764	22.008067090334002
19	22.704796726734568	26.767070021183365	30.66538986100229	19.86274339107977
20	24.79208380488088	25.94178926902876	29.327065378253682	19.939061547836683
21	26.35384927889498	24.750297437683177	28.12715823684745	20.76869504657439
22	24.056179449232467	26.969617828850005	28.18693595658861	20.787266765328923
23	22.986274339107975	26.634746525057313	28.901366762427095	21.47761237340762
24	21.79045573917964	27.299661931776964	29.13712801613442	21.772754312908983
25	22.029314382386993	27.03643839830068	29.8555754492773	21.078671770035026
26	20.72854004706811	27.274257342421947	30.517081777212884	21.480120833297057
27	21.345702943634507	28.45784195375615	29.033858761259168	21.16259634135017
28	20.984605562890845	27.36640501443682	29.654241813090348	21.994747609581985
29	21.415637477945957	27.614274770173648	29.688805831553534	21.281281920326865
30	22.277454701628535	26.91524184281088	29.887929333379766	20.919374122180823
31	21.568502273591232	26.99465190984565	29.626620314617437	21.81022550194568
32	20.662147495980918	27.811181465209543	29.5653586995003	21.96131233930924
33	21.127712857180168	27.468522296283616	29.585414194057613	21.818350652478607
34	21.221152088308777	27.56225171135983	30.42520653842471	20.791389661906685
35	22.443051565537857	27.47192478453904	28.820115492876003	21.264908157047095
36	21.157250224891907	27.541568729868548	29.350860392907922	21.95032065233162
37	22.245018180340857	27.084500134937883	28.117282786278704	22.553198898442556
38	21.26374742462494	27.774585763616845	29.268158207829142	21.69350860392908
39	21.2595797531681	28.726636429323836	28.166868345680733	21.846915471827327
40	22.05781641749466	27.822047079580276	29.586660785588265	20.5334757173368
41	21.91430213054909	27.869799132922818	29.19595131831715	21.019947418210943
42	21.274416202114356	28.449754350734334	28.286955487134247	21.988873960017063
43	20.836864037887846	28.432135021880693	28.673286979535455	22.057713960696006
44	21.586209898576588	28.009112146142574	28.571801680233317	21.832876275047518
45	22.046140452698783	28.056877539175858	28.72954149738828	21.167440510737087
46	22.69655226448986	27.83986210218715	27.24845691999199	22.21512871333099
47	22.193235733666082	28.4499644520538	27.12903179002046	22.22776802425966
48	21.84718606804048	28.75686687619164	27.422813844899284	21.9731332108686
49	21.85893045769539	28.307061022182502	27.548201372041465	22.28580714808065
50	21.541662555643903	28.60343236545348	27.591250195878143	22.263654883024476
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	100.0
1	57.5
2	15.0
3	62.5
4	110.0
5	116.0
6	122.0
7	121.5
8	121.0
9	166.5
10	212.0
11	382.5
12	553.0
13	1059.0
14	1565.0
15	2228.0
16	2891.0
17	3235.5
18	3580.0
19	3406.5
20	3233.0
21	3394.5
22	3556.0
23	3387.5
24	3219.0
25	3884.5
26	4550.0
27	5873.0
28	7196.0
29	8292.0
30	9388.0
31	9823.5
32	10259.0
33	11567.0
34	12875.0
35	14746.0
36	16617.0
37	19626.5
38	22636.0
39	22855.0
40	23074.0
41	24463.5
42	25853.0
43	24744.0
44	23635.0
45	24190.0
46	24745.0
47	24385.5
48	24026.0
49	23165.0
50	22304.0
51	21614.0
52	20924.0
53	19644.5
54	18365.0
55	16764.0
56	15163.0
57	14086.0
58	13009.0
59	11169.0
60	9329.0
61	7935.0
62	6541.0
63	5370.0
64	4199.0
65	3716.0
66	3233.0
67	2795.0
68	2357.0
69	2062.5
70	1768.0
71	1436.0
72	1104.0
73	941.0
74	778.0
75	674.5
76	571.0
77	454.0
78	337.0
79	291.0
80	245.0
81	182.0
82	119.0
83	100.0
84	81.0
85	54.5
86	28.0
87	18.5
88	9.0
89	6.0
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	2.0
98	3.0
99	4.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03279069092597429
2	0.0046429296886335274
3	0.0
4	0.0
5	0.0
6	2.9018310553959546E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0011607324221583819
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0014509155276979774
25	2.9018310553959546E-4
26	8.705493166187865E-4
27	5.803662110791909E-4
28	0.0014509155276979774
29	5.803662110791909E-4
30	0.001741098633237573
31	2.9018310553959546E-4
32	0.0011607324221583819
33	2.9018310553959546E-4
34	2.9018310553959546E-4
35	0.0
36	0.0
37	0.002031281738777168
38	0.0
39	8.705493166187865E-4
40	5.803662110791909E-4
41	0.0011607324221583819
42	0.003772380372014741
43	0.004062563477554336
44	0.004352746583093933
45	0.002901831055395955
46	0.002031281738777168
47	0.0014509155276979774
48	0.0060938452163315044
49	0.004062563477554336
50	0.0023214648443167637
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	344610.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.14921899335484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.21278070043154	42.79288113641058
2	14.175893049240656	15.919306464963807
3	4.66080705127388	7.851020274232487
4	1.8891989828040399	4.24308189629949
5	0.9113860882717418	2.558680852893353
6	0.4981284578133828	1.6781714318751448
7	0.31767798771676226	1.248615963117374
8	0.19900032416746344	0.8938970225142009
9	0.1329732640036687	0.6719710428722864
>10	0.8190628818027247	9.007597438596536
>50	0.11047713387250675	4.401654323887777
>100	0.07053939064159984	7.88418591083029
>500	0.002074687960047054	0.8489362415067336
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	934	0.2710310205739822	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	880	0.255361132874844	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	583	0.16917675052958417	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	518	0.15031484866951045	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	486	0.1410289892922434	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	446	0.12942166507065958	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	437	0.12681001712080323	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	431	0.12506891848756566	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	423	0.12274745364324888	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	412	0.11955543948231333	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC	408	0.11839470706015497	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	405	0.11752415774353617	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	401	0.11636342532137778	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	386	0.11201067873828387	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG	379	0.10997939699950668	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	373	0.10823829836626912	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	369	0.10707756594411073	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	367	0.10649719973303154	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA	362	0.10504628420533357	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	359	0.10417573488871479	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	352	0.1021444531499376	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	2.9018310553959546E-4	0.0
19	0.0	0.0	0.0	2.9018310553959546E-4	0.0
20	0.0	0.0	0.0	5.803662110791909E-4	0.0
21	0.0	0.0	0.0	5.803662110791909E-4	0.0
22	0.0	0.0	0.0	5.803662110791909E-4	0.0
23	0.0	0.0	0.0	0.0020312817387771686	0.0
24	0.0	0.0	0.0	0.003772380372014741	0.0
25	0.0	0.0	0.0	0.004352746583093932	0.0
26	0.0	0.0	0.0	0.005513479005252314	0.0
27	0.0	0.0	0.0	0.00580366211079191	0.0
28	0.0	0.0	0.0	0.007834943849569079	0.0
29	0.0	0.0	0.0	0.012477873538202606	0.0
30	0.0	0.0	0.0	0.018861901860073707	0.0
31	0.0	0.0	0.0	0.0325005078204347	0.0
32	0.0	0.0	0.0	0.05194277589158759	0.0
33	0.0	0.0	0.0	0.06703229737964655	0.0
34	0.0	0.0	0.0	0.08473346681756189	0.0
35	0.0	0.0	0.0	0.10533646731087316	0.0
36	0.0	0.0	0.0	0.13754679202576825	0.0
37	0.0	0.0	0.0	0.17556077885145527	0.0
38	0.0	0.0	0.0	0.2248919067931865	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGGAC	55	4.9516148E-6	27.997046	3
GTTTGTC	45	0.0013970471	24.441864	9
GCGTGAA	45	0.0013970471	24.441864	18
TAGGACG	90	1.5063051E-8	24.441864	4
GTATCAA	1920	0.0	23.719688	1
GGTATCA	925	0.0	23.30903	1
GTTACTC	70	3.205779E-5	22.00087	35
AAAGCGT	80	3.6064375E-6	21.997679	10
TGTAGGA	165	0.0	21.331081	2
ACGATTA	75	5.4564298E-5	20.534147	40
CGTATGC	75	5.4625314E-5	20.531166	9
GCGTATG	75	5.4625314E-5	20.531166	8
GTTATAT	110	1.2585406E-7	20.000792	1
ATAGCCC	110	1.2604687E-7	19.99789	3
GTATAGC	155	3.274181E-11	19.871756	1
TCCTCGC	90	9.982157E-6	19.55633	44
TTACTCA	90	9.982157E-6	19.55633	36
AGCGTTC	90	9.994677E-6	19.553492	12
GAAAGCG	90	9.994677E-6	19.553492	9
GACGTGG	80	8.972102E-5	19.247969	7
>>END_MODULE