FastQCFastQC Report
Thu 2 Feb 2017
SRR4062087_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062087_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543150
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23220.1504714382918057No Hit
TATCAACGCAGAGTACTTTTTTTTT15950.1033600103684023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTG400.005279133614.2499765
GGTATCA7500.013.7981311
TAGCACC1257.2759576E-1213.6782014
CCGCTCA604.08533E-412.6691129
CGCAATA853.959096E-612.2897072
GTCTATA853.970603E-612.2865181
GTATCAA18100.012.1692681
GTTCTAG1103.846253E-812.0834351
CCGACCA1900.012.0023179
CGGTCCA3000.011.71892910
CCTAATA658.047534E-411.6881132
CGACCAT2050.011.58760410
CGCCGGT2800.011.5379417
CAAGACG3150.011.458814
GACCGAG855.312097E-511.1786297
TTAGAAC855.3332195E-511.1742743
CGAACGA600.00587143611.084393516
CCGTTAA600.00587143611.084393516
TGGACGG600.005875694611.0833145
TCGCGTA1553.110472E-1011.03438959
Produced by FastQC (version 0.11.5)