##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062087_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1543150
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.162570715743772	32.0	32.0	32.0	32.0	32.0
2	30.60800505459612	32.0	32.0	32.0	32.0	32.0
3	30.641362148851375	32.0	32.0	32.0	32.0	32.0
4	30.65331756472151	32.0	32.0	32.0	32.0	32.0
5	30.529029582347796	32.0	32.0	32.0	32.0	32.0
6	34.181380941580535	36.0	36.0	36.0	32.0	36.0
7	34.107563101448335	36.0	36.0	36.0	32.0	36.0
8	34.04404432491981	36.0	36.0	36.0	32.0	36.0
9	34.21601205326767	36.0	36.0	36.0	32.0	36.0
10	33.841357612675374	36.0	36.0	36.0	32.0	36.0
11	34.21043450085863	36.0	36.0	36.0	32.0	36.0
12	33.98734666105045	36.0	36.0	36.0	32.0	36.0
13	34.09124582833814	36.0	36.0	36.0	32.0	36.0
14	33.97764054045297	36.0	36.0	36.0	32.0	36.0
15	33.91310760457506	36.0	36.0	36.0	32.0	36.0
16	33.92684508958948	36.0	36.0	36.0	32.0	36.0
17	33.84600784110423	36.0	36.0	36.0	32.0	36.0
18	33.84671742863623	36.0	36.0	36.0	32.0	36.0
19	33.85561675793021	36.0	36.0	36.0	32.0	36.0
20	33.849188996533066	36.0	36.0	36.0	32.0	36.0
21	33.83428765836114	36.0	36.0	36.0	32.0	36.0
22	33.795827366101804	36.0	36.0	36.0	32.0	36.0
23	33.75882642646535	36.0	36.0	36.0	32.0	36.0
24	33.733994102971195	36.0	36.0	36.0	32.0	36.0
25	33.35636522697081	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	31.0
5	103.0
6	318.0
7	80.0
8	275.0
9	195.0
10	121.0
11	33.0
12	60.0
13	82.0
14	208.0
15	296.0
16	527.0
17	703.0
18	1004.0
19	1404.0
20	2349.0
21	3585.0
22	5465.0
23	7973.0
24	11468.0
25	15823.0
26	21818.0
27	27769.0
28	36393.0
29	47609.0
30	61306.0
31	80845.0
32	113155.0
33	154306.0
34	320598.0
35	627244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.2686081625776	17.396801327164095	11.740088391202354	25.59450211905595
2	16.9470678730334	19.118994573423382	37.328224289859804	26.605713263683413
3	19.478957474287768	22.59131885244149	28.00380005145363	29.92592362181712
4	13.1657604292908	15.361015158683353	34.720124950583596	36.75309946144225
5	15.216431625108987	36.10568929137482	33.12747344640826	15.550405637107936
6	35.431040296308595	34.28214419106509	16.2561212209242	14.030694291702114
7	31.149520057552877	29.89570812026163	19.64731349644738	19.30745832573811
8	28.476602944191153	33.24845797145074	18.514073524336418	19.760865560021692
9	26.70845776696163	14.906278202342543	18.516567325216034	39.86869670547979
10	16.906536440522896	26.64248069902681	30.48132714387125	25.969655716579048
11	37.220527103371396	21.389386032422557	21.533770358189933	19.85631650601612
12	24.632669746677742	23.457420577242523	28.251985568936878	23.657924107142858
13	29.56937966690285	19.2914872577493	24.769266506269254	26.3698665690786
14	23.563403701514694	19.46451498635304	24.73379184348459	32.23828946864767
15	25.31230290461816	26.85753069821404	21.817274574793075	26.012891822374723
16	25.965551068711008	25.87932493174575	23.107631499724906	25.047492499818336
17	24.28182780217734	26.159797409746314	24.592032773632123	24.966342014444223
18	25.026144859388666	25.238936767068115	25.418966506034728	24.315951867508492
19	25.722117788389813	25.08649845684418	24.841889772360194	24.349493982405818
20	25.777831975190846	24.561568513493967	24.60191387497738	25.058685636337806
21	26.529371932045787	24.296381240984235	24.40113274317915	24.773114083790823
22	26.124890695990842	24.259522098221815	24.60254856730662	25.013038638480722
23	24.713823755644075	24.413022527015684	25.19103277682728	25.68212094051296
24	24.85433769785134	24.893643655817314	24.954224125685663	25.297794520645684
25	25.095847940509742	24.45204035634484	24.898801049446575	25.553310653698848
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	270.0
1	270.0
2	321.5
3	373.0
4	373.0
5	373.0
6	865.5
7	1358.0
8	1358.0
9	1358.0
10	1751.0
11	2144.0
12	2144.0
13	2144.0
14	2953.0
15	3762.0
16	3762.0
17	3762.0
18	6333.0
19	8904.0
20	8904.0
21	8904.0
22	14081.0
23	19258.0
24	19258.0
25	19258.0
26	28399.0
27	37540.0
28	37540.0
29	37540.0
30	48064.0
31	58588.0
32	58588.0
33	58588.0
34	73980.5
35	89373.0
36	89373.0
37	89373.0
38	105865.5
39	122358.0
40	122358.0
41	122358.0
42	141792.5
43	161227.0
44	161227.0
45	161227.0
46	179779.0
47	198331.0
48	198331.0
49	198331.0
50	205913.0
51	213495.0
52	213495.0
53	213495.0
54	202648.5
55	191802.0
56	191802.0
57	191802.0
58	175725.5
59	159649.0
60	159649.0
61	159649.0
62	140200.0
63	120751.0
64	120751.0
65	120751.0
66	98285.0
67	75819.0
68	75819.0
69	75819.0
70	57538.5
71	39258.0
72	39258.0
73	39258.0
74	29418.0
75	19578.0
76	19578.0
77	19578.0
78	15517.0
79	11456.0
80	11456.0
81	11456.0
82	7930.0
83	4404.0
84	4404.0
85	4404.0
86	3182.0
87	1960.0
88	1960.0
89	1960.0
90	1327.0
91	694.0
92	694.0
93	694.0
94	443.0
95	192.0
96	192.0
97	192.0
98	399.0
99	606.0
100	606.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.480251433755629E-4
2	2.592100573502252E-4
3	8.424326863882319E-4
4	0.00907235200725788
5	0.0333732948838415
6	0.06311764896477984
7	0.1043968505978032
8	0.10847940900106925
9	0.11878300878074069
10	0.13193791919126463
11	0.13764054045296958
12	0.1315491041052393
13	0.12817937335968638
14	0.11541327803518778
15	0.1307714739331886
16	0.12014386158182937
17	0.12383760489907009
18	0.11262676991867285
19	0.09746298156368467
20	0.09467647344716976
21	0.09111233515860416
22	0.10225836762466384
23	0.08216958818002139
24	0.09065871755824126
25	0.08975148235751548
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1543150.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.34763079958872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.09178724686238	42.11500147420193
2	13.913012158311277	15.401045204968034
3	4.4779805345473624	7.435368400616172
4	1.9359083162365103	4.285917549956474
5	0.9719859688859793	2.6898560274140855
6	0.608053613346907	2.019259613872829
7	0.39536493383499965	1.5317758672302366
8	0.2739539558897593	1.2130161925338572
9	0.20644704881904813	1.0283719533931208
>10	0.9882978046852469	9.763865044689943
>50	0.07206524066457061	2.7730424026026808
>100	0.05785296957943495	6.429673723996299
>500	0.00540886424968009	1.988843614380874
>1k	0.0018813440868452487	1.3249629301433317
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2322	0.1504714382918057	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1595	0.1033600103684023	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9440754301266888E-4
2	0.0	0.0	0.0	0.0	1.9440754301266888E-4
3	0.0	0.0	0.0	0.0	1.9440754301266888E-4
4	0.0	0.0	0.0	0.0	1.9440754301266888E-4
5	0.0	0.0	0.0	0.0	1.9440754301266888E-4
6	0.0	0.0	0.0	0.0	3.2401257168778147E-4
7	0.0	0.0	0.0	0.0	3.2401257168778147E-4
8	0.0	0.0	0.0	0.0	3.2401257168778147E-4
9	0.0	0.0	0.0	6.48025143375563E-5	3.2401257168778147E-4
10	0.0	0.0	0.0	6.48025143375563E-5	3.8881508602533777E-4
11	0.0	0.0	0.0	6.48025143375563E-5	3.8881508602533777E-4
12	0.0	0.0	0.0	6.48025143375563E-5	7.776301720506755E-4
13	0.0	0.0	0.0	6.48025143375563E-5	9.072352007257881E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTG	40	0.0052791336	14.249976	5
GGTATCA	750	0.0	13.798131	1
TAGCACC	125	7.2759576E-12	13.678201	4
CCGCTCA	60	4.08533E-4	12.669112	9
CGCAATA	85	3.959096E-6	12.289707	2
GTCTATA	85	3.970603E-6	12.286518	1
GTATCAA	1810	0.0	12.169268	1
GTTCTAG	110	3.846253E-8	12.083435	1
CCGACCA	190	0.0	12.002317	9
CGGTCCA	300	0.0	11.718929	10
CCTAATA	65	8.047534E-4	11.688113	2
CGACCAT	205	0.0	11.587604	10
CGCCGGT	280	0.0	11.537941	7
CAAGACG	315	0.0	11.45881	4
GACCGAG	85	5.312097E-5	11.178629	7
TTAGAAC	85	5.3332195E-5	11.174274	3
CGAACGA	60	0.005871436	11.0843935	16
CCGTTAA	60	0.005871436	11.0843935	16
TGGACGG	60	0.0058756946	11.083314	5
TCGCGTA	155	3.110472E-10	11.0343895	9
>>END_MODULE