FastQCFastQC Report
Thu 2 Feb 2017
SRR4062087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543150
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8100.026.0796451
GTATCAA17050.025.2957251
TATACTG2250.019.5546635
GTATTAG1705.456968E-1219.415911
TCAACGC24200.017.7264634
ATCAACG24800.017.2975983
GTATTAC1408.3560735E-817.2894081
ACCGTCG3950.017.2650348
CAACGCA25400.016.8889925
TATCAAC26450.016.5517652
CGTCGTA4000.016.49924710
ATACCGT4400.016.4992476
GTAAACG2700.016.2966127
GTCCTAT3650.016.2774491
AACGCAG26600.016.2097856
CGCTTCG3100.015.61269732
TACCGTC4100.015.5602657
CCGTCGT4300.015.3481379
GTACTAG1158.204916E-515.3076161
CTAGATA3050.015.146853