##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062087_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1543150
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2488967371934	32.0	32.0	32.0	32.0	32.0
2	31.399386320189222	32.0	32.0	32.0	32.0	32.0
3	31.549441726338983	32.0	32.0	32.0	32.0	32.0
4	31.63307714739332	32.0	32.0	32.0	32.0	32.0
5	31.607184006739462	32.0	32.0	32.0	32.0	32.0
6	35.24027217056022	36.0	36.0	36.0	36.0	36.0
7	35.25063733272851	36.0	36.0	36.0	36.0	36.0
8	35.19160483426757	36.0	36.0	36.0	36.0	36.0
9	35.28834915594725	36.0	36.0	36.0	36.0	36.0
10	35.16103878430483	36.0	36.0	36.0	36.0	36.0
11	35.286157534912356	36.0	36.0	36.0	36.0	36.0
12	35.21131257492791	36.0	36.0	36.0	36.0	36.0
13	35.24863299096005	36.0	36.0	36.0	36.0	36.0
14	35.20943913423841	36.0	36.0	36.0	36.0	36.0
15	35.185743446845734	36.0	36.0	36.0	36.0	36.0
16	35.195419110261476	36.0	36.0	36.0	36.0	36.0
17	35.17271101318731	36.0	36.0	36.0	36.0	36.0
18	35.17482875935586	36.0	36.0	36.0	36.0	36.0
19	35.16367235848751	36.0	36.0	36.0	36.0	36.0
20	35.166352590480514	36.0	36.0	36.0	36.0	36.0
21	35.156807828143734	36.0	36.0	36.0	36.0	36.0
22	35.14017367073843	36.0	36.0	36.0	36.0	36.0
23	35.11082137186923	36.0	36.0	36.0	36.0	36.0
24	35.08938794025208	36.0	36.0	36.0	36.0	36.0
25	35.07660953244986	36.0	36.0	36.0	36.0	36.0
26	35.030880342157275	36.0	36.0	36.0	36.0	36.0
27	35.01955804685222	36.0	36.0	36.0	36.0	36.0
28	34.99759647474322	36.0	36.0	36.0	36.0	36.0
29	34.97749473479571	36.0	36.0	36.0	36.0	36.0
30	34.96135242847423	36.0	36.0	36.0	36.0	36.0
31	34.96475715257752	36.0	36.0	36.0	36.0	36.0
32	34.93630301655704	36.0	36.0	36.0	36.0	36.0
33	34.91715776172116	36.0	36.0	36.0	32.0	36.0
34	34.90808281761333	36.0	36.0	36.0	32.0	36.0
35	34.89468036159803	36.0	36.0	36.0	32.0	36.0
36	34.86959336422253	36.0	36.0	36.0	32.0	36.0
37	34.864897126008486	36.0	36.0	36.0	32.0	36.0
38	34.84545896380779	36.0	36.0	36.0	32.0	36.0
39	34.82782425558111	36.0	36.0	36.0	32.0	36.0
40	34.81319055179341	36.0	36.0	36.0	32.0	36.0
41	34.784238084437675	36.0	36.0	36.0	32.0	36.0
42	34.76414152869131	36.0	36.0	36.0	32.0	36.0
43	34.752110293879404	36.0	36.0	36.0	32.0	36.0
44	34.71469980235233	36.0	36.0	36.0	32.0	36.0
45	34.69849334154165	36.0	36.0	36.0	32.0	36.0
46	34.68362310857661	36.0	36.0	36.0	32.0	36.0
47	34.656284871853025	36.0	36.0	36.0	32.0	36.0
48	34.63077212195833	36.0	36.0	36.0	32.0	36.0
49	34.614854680361596	36.0	36.0	36.0	32.0	36.0
50	34.189265463499986	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	13.0
21	40.0
22	108.0
23	294.0
24	677.0
25	1683.0
26	3577.0
27	6798.0
28	11880.0
29	19061.0
30	29027.0
31	43263.0
32	65780.0
33	113966.0
34	260605.0
35	986376.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.160274020554134	17.831884967374492	12.141706068357342	25.86613494371403
2	16.324028320075172	19.576754208317805	37.04201027169775	27.05720719990927
3	18.866547775191428	22.986902064875345	28.359051391185787	29.78749876874744
4	12.8915529922561	15.853157502511097	34.854745164112366	36.40054434112044
5	14.905291125295662	36.481158668956354	32.96452062340019	15.649029582347795
6	35.59213736604484	34.39050200661376	16.170073233463825	13.847287393877576
7	31.139357807082913	29.968376373003274	19.720636360690794	19.17162945922302
8	28.4884813530765	33.425784920454916	18.482195509185757	19.603538217282832
9	26.996014645368238	14.596636749505882	18.35213686291028	40.0552117422156
10	16.825411431696764	26.665815592541996	30.573995494923366	25.934777480837873
11	37.37361889641318	21.255224702718465	21.48708809901824	19.88406830185011
12	24.586786767326572	23.405890548553284	28.300878074069274	23.70644461005087
13	29.758740239121277	19.16890775362084	24.90827204095519	26.164079966302694
14	23.713119269027636	19.24530991802482	24.801542299841234	32.2400285131063
15	25.37284126624113	26.863039885947575	21.721478793377184	26.042640054434113
16	26.057823470898718	25.765044762481814	23.186997981395137	24.99013378522433
17	24.5493957165538	26.013932540582573	24.528140491851083	24.90853125101254
18	25.08229929987273	25.04387135906601	25.565921091172072	24.307908249889188
19	25.814146388879887	25.081748371836827	24.810809059391502	24.29329617989178
20	25.91410782143468	24.40504580259522	24.692770084969386	24.98807629100071
21	26.652319380694927	24.18891500431909	24.372241436180175	24.786524178805806
22	26.14625927485986	24.15669247966821	24.690146777694974	25.00690146777695
23	24.731636413593243	24.256244860347252	25.344603793930464	25.667514932129045
24	25.021255472636174	24.751673868470096	25.04613992840535	25.18093073048838
25	25.293377753383183	24.26483024624355	25.02846455976002	25.413327440613244
26	24.864885882032738	25.142890470080488	25.329522920797853	24.662700727088925
27	25.494558191226357	24.74440185465397	24.71400937695421	25.047030577165465
28	25.122753674185795	24.538292601211435	25.1844464274258	25.154507297176977
29	24.933058568892	24.82561530386096	25.220978005884103	25.020348121362936
30	25.024592872927105	24.872499400568973	25.178824855974547	24.92408287052938
31	25.422497561797496	24.67901590582868	24.632033930706445	25.26645260166737
32	25.226016873420832	24.701565706738933	24.463349657224214	25.60906776261602
33	24.873521446757827	24.50077665964422	25.05133989179255	25.5743620018054
34	25.550436123747684	24.599841880840366	25.12662493357699	24.72309706183496
35	25.764492948500695	24.40908092359315	25.14303562784362	24.683390500062536
36	24.832728401469982	24.94224472166978	24.90511285689198	25.31991401996826
37	25.690869454769793	24.74610012908845	24.714670461556008	24.84835995458575
38	25.109500119560717	24.49108932449167	25.083903109809874	25.315507446137737
39	25.719084828227086	24.528656468426107	24.51854722378109	25.233711479565716
40	25.826005249003664	24.700515179988983	24.730972361727634	24.742507209279722
41	24.857481130871108	25.012037230847195	25.268399466279227	24.86208217200247
42	25.8548901459601	24.86998515319556	24.778610232824594	24.49651446801975
43	25.454023480129507	24.229339533870956	24.958460027010705	25.35817695898884
44	25.030669280495115	24.803136594138326	24.761725774832723	25.404468350533836
45	25.158266709782694	24.942988990508212	24.80255948488876	25.096184814820333
46	25.15194933877169	24.85877573450551	24.659308812770032	25.329966113952768
47	25.184495388587337	24.75769994452826	25.095390661046913	24.962414005837495
48	25.88610933617784	25.123680086890367	24.119787906634333	24.87042267029747
49	25.127099449608348	25.282697042220025	24.33174365863664	25.258459849534994
50	25.236923212512863	25.262844789374228	24.433613545579206	25.066618452533707
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	115.0
1	73.5
2	32.0
3	106.0
4	180.0
5	178.5
6	177.0
7	149.5
8	122.0
9	171.5
10	221.0
11	367.5
12	514.0
13	949.5
14	1385.0
15	1813.5
16	2242.0
17	2331.5
18	2421.0
19	2446.5
20	2472.0
21	2971.0
22	3470.0
23	4330.5
24	5191.0
25	6645.0
26	8099.0
27	10364.5
28	12630.0
29	14948.5
30	17267.0
31	19646.0
32	22025.0
33	25669.0
34	29313.0
35	33923.0
36	38533.0
37	45319.5
38	52106.0
39	57850.0
40	63594.0
41	70186.0
42	76778.0
43	81352.5
44	85927.0
45	93642.5
46	101358.0
47	107318.5
48	113279.0
49	118605.0
50	123931.0
51	121575.0
52	119219.0
53	119300.0
54	119381.0
55	118335.0
56	117289.0
57	112831.0
58	108373.0
59	99671.0
60	90969.0
61	79982.5
62	68996.0
63	59284.5
64	49573.0
65	41739.0
66	33905.0
67	28952.5
68	24000.0
69	21002.0
70	18004.0
71	14136.5
72	10269.0
73	9216.5
74	8164.0
75	6043.5
76	3923.0
77	3493.0
78	3063.0
79	2622.5
80	2182.0
81	1636.0
82	1090.0
83	885.5
84	681.0
85	554.5
86	428.0
87	282.5
88	137.0
89	90.0
90	43.0
91	32.5
92	22.0
93	20.5
94	19.0
95	18.5
96	18.0
97	17.0
98	16.0
99	10.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0318828370540777
2	0.004860188575316722
3	3.8881508602533777E-4
4	0.0
5	0.0
6	1.9440754301266888E-4
7	0.0
8	0.0
9	0.0
10	1.296050286751126E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.2401257168778147E-4
17	0.0
18	1.296050286751126E-4
19	0.0
20	3.8881508602533777E-4
21	6.48025143375563E-5
22	0.0
23	6.48025143375563E-5
24	0.0011664452580760134
25	1.9440754301266888E-4
26	6.480251433755629E-4
27	3.2401257168778147E-4
28	0.0012312477724135697
29	6.480251433755629E-4
30	0.0012960502867511259
31	3.2401257168778147E-4
32	8.424326863882319E-4
33	1.9440754301266888E-4
34	6.480251433755629E-4
35	1.9440754301266888E-4
36	6.48025143375563E-5
37	0.0014256553154262386
38	6.48025143375563E-5
39	5.184201147004504E-4
40	0.0
41	0.0013608528010886822
42	0.003693743317240709
43	0.003758545831578265
44	0.004860188575316722
45	0.0017496678871140202
46	0.0023976930304895833
47	0.0014256553154262386
48	0.0036289408029031526
49	0.004341768460616272
50	0.002203285487476914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1543150.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.92819396387089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.13936724589738	42.02409105651076
2	14.607878881308936	16.33984566949159
3	4.659489149760133	7.8179043882100965
4	1.9848892352103398	4.440450805745729
5	1.0270171366654979	2.871960681182363
6	0.6141362705290576	2.0608519475038456
7	0.4028195779724655	1.5770280042502065
8	0.27953450116609985	1.250708784064922
9	0.2051450064035898	1.0326050737973553
>10	0.9549444995227134	9.454766339831385
>50	0.06752445790023291	2.5867806811839698
>100	0.050853856113988564	5.7237876810922
>500	0.005120145239769288	1.9577945095067808
>1k	0.001280036309942322	0.8614243776288728
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.48025143375563E-5
2	0.0	0.0	0.0	0.0	6.48025143375563E-5
3	0.0	0.0	0.0	0.0	6.48025143375563E-5
4	0.0	0.0	0.0	0.0	6.48025143375563E-5
5	0.0	0.0	0.0	0.0	6.48025143375563E-5
6	0.0	0.0	0.0	0.0	1.296050286751126E-4
7	0.0	0.0	0.0	0.0	1.296050286751126E-4
8	0.0	0.0	0.0	0.0	1.296050286751126E-4
9	0.0	0.0	0.0	0.0	1.296050286751126E-4
10	0.0	0.0	0.0	0.0	1.296050286751126E-4
11	0.0	0.0	0.0	0.0	1.296050286751126E-4
12	0.0	0.0	0.0	0.0	5.184201147004504E-4
13	0.0	0.0	0.0	0.0	6.480251433755629E-4
14	0.0	0.0	0.0	0.0	6.480251433755629E-4
15	0.0	0.0	0.0	6.48025143375563E-5	0.0010368402294009009
16	0.0	0.0	0.0	1.296050286751126E-4	0.0010368402294009009
17	0.0	0.0	0.0	1.296050286751126E-4	0.0010368402294009009
18	0.0	0.0	0.0	1.9440754301266888E-4	0.0010368402294009009
19	0.0	0.0	0.0	1.9440754301266888E-4	0.0010368402294009009
20	0.0	0.0	0.0	3.2401257168778147E-4	0.0011664452580760134
21	0.0	0.0	0.0	4.536176003628941E-4	0.0011664452580760134
22	0.0	0.0	0.0	5.184201147004504E-4	0.0011664452580760134
23	0.0	0.0	0.0	7.128276577131193E-4	0.0011664452580760134
24	0.0	0.0	0.0	0.0010368402294009009	0.0011664452580760134
25	0.0	0.0	0.0	0.0016200628584389074	0.0011664452580760134
26	0.0	0.0	0.0	0.002138482973139358	0.0012960502867511259
27	0.0	0.0	0.0	0.0024624955448271394	0.0012960502867511259
28	0.0	0.0	0.0	0.0033697307455529276	0.0012960502867511259
29	0.0	0.0	0.0	0.00421216343194116	0.0012960502867511259
30	0.0	0.0	0.0	0.005119398632666947	0.0012960502867511259
31	0.0	0.0	0.0	0.007646696691831643	0.0012960502867511259
32	0.0	0.0	0.0	0.01069241486569679	0.0013608528010886822
33	0.0	0.0	0.0	0.013543725496549266	0.0013608528010886822
34	0.0	0.0	0.0	0.01678385121342708	0.001490457829763795
35	0.0	0.0	0.0	0.02132002721705602	0.001490457829763795
36	0.0	0.0	0.0	0.02611541327803519	0.001490457829763795
37	0.0	0.0	0.0	0.03389171499854194	0.001490457829763795
38	0.0	0.0	0.0	0.043288079577487606	0.001555260344101351
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	810	0.0	26.079645	1
GTATCAA	1705	0.0	25.295725	1
TATACTG	225	0.0	19.554663	5
GTATTAG	170	5.456968E-12	19.41591	1
TCAACGC	2420	0.0	17.726463	4
ATCAACG	2480	0.0	17.297598	3
GTATTAC	140	8.3560735E-8	17.289408	1
ACCGTCG	395	0.0	17.265034	8
CAACGCA	2540	0.0	16.888992	5
TATCAAC	2645	0.0	16.551765	2
CGTCGTA	400	0.0	16.499247	10
ATACCGT	440	0.0	16.499247	6
GTAAACG	270	0.0	16.29661	27
GTCCTAT	365	0.0	16.277449	1
AACGCAG	2660	0.0	16.209785	6
CGCTTCG	310	0.0	15.612697	32
TACCGTC	410	0.0	15.560265	7
CCGTCGT	430	0.0	15.348137	9
GTACTAG	115	8.204916E-5	15.307616	1
CTAGATA	305	0.0	15.14685	3
>>END_MODULE