FastQCFastQC Report
Thu 2 Feb 2017
SRR4062086_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062086_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992971
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17620.17744727690939616No Hit
TATCAACGCAGAGTACTTTTTTTTT12580.12669050757776412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCG704.4251465E-714.9303949
AGGCGGT456.7530223E-414.77958111
CGAACGA902.2846507E-914.77734616
AACCGCG653.3648848E-614.6171697
CGCCGGT1450.013.7603017
CGGTCCA1550.013.485517510
GGTATCA4650.013.4814391
CTATTGT500.0015009213.2976011
CGGTCGG751.47545215E-512.66821310
GTTACAC604.0924526E-412.6656583
CTTATAC701.0909433E-412.2133143
CGATAAC1103.8007784E-812.09238510
AAACTAT550.00306545312.0917766
TAAACTA550.003066610812.0911665
ATAATAC550.003068928112.0899473
GGTCGGC951.0369713E-612.00146511
GAACCGC951.0376025E-612.000866
GTATCAA11950.011.9225351
ACGAGAC1201.0004442E-811.87405519
TAACGAA1302.6102498E-911.69196613
Produced by FastQC (version 0.11.5)