##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062086_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992971
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30116891631276	32.0	32.0	32.0	32.0	32.0
2	30.893406756088545	32.0	32.0	32.0	32.0	32.0
3	30.912120293543317	32.0	32.0	32.0	32.0	32.0
4	30.913086082070876	32.0	32.0	32.0	32.0	32.0
5	30.854335121569513	32.0	32.0	32.0	32.0	32.0
6	34.5146283224787	36.0	36.0	36.0	32.0	36.0
7	34.45069694885349	36.0	36.0	36.0	32.0	36.0
8	34.41153367016761	36.0	36.0	36.0	32.0	36.0
9	34.530667058755995	36.0	36.0	36.0	32.0	36.0
10	34.254800996202306	36.0	36.0	36.0	32.0	36.0
11	34.515620295053935	36.0	36.0	36.0	32.0	36.0
12	34.35141711087232	36.0	36.0	36.0	32.0	36.0
13	34.4335635179678	36.0	36.0	36.0	32.0	36.0
14	34.34008344654577	36.0	36.0	36.0	32.0	36.0
15	34.29948508063176	36.0	36.0	36.0	32.0	36.0
16	34.30122128440811	36.0	36.0	36.0	32.0	36.0
17	34.24725193384298	36.0	36.0	36.0	32.0	36.0
18	34.24293861552855	36.0	36.0	36.0	32.0	36.0
19	34.248282175410964	36.0	36.0	36.0	32.0	36.0
20	34.23431097182093	36.0	36.0	36.0	32.0	36.0
21	34.222825238602134	36.0	36.0	36.0	32.0	36.0
22	34.19211840023525	36.0	36.0	36.0	32.0	36.0
23	34.1600469701532	36.0	36.0	36.0	32.0	36.0
24	34.14628725310205	36.0	36.0	36.0	32.0	36.0
25	33.804112103978866	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	8.0
5	74.0
6	220.0
7	42.0
8	160.0
9	124.0
10	73.0
11	25.0
12	40.0
13	44.0
14	147.0
15	248.0
16	349.0
17	437.0
18	673.0
19	840.0
20	1194.0
21	1729.0
22	2503.0
23	3725.0
24	5126.0
25	7612.0
26	10487.0
27	13290.0
28	17865.0
29	23660.0
30	31679.0
31	43631.0
32	61971.0
33	89846.0
34	202774.0
35	472374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86747730527452	17.56159853045736	12.143163585313436	25.427760578954683
2	16.847756576489296	19.055881905678827	37.42684816947961	26.669513348352265
3	19.618778871805407	22.250588388133743	28.034768527050748	30.0958642130101
4	13.304165571364832	15.29585235633417	34.49238928890487	36.90759278339613
5	15.532675820799291	35.758137473177314	32.93926236361988	15.769924342403513
6	35.617695933152014	33.919086281832556	16.25509779400428	14.208119991011154
7	31.3693644648762	29.860270989595932	19.436849745947253	19.333514799580612
8	28.527329659672198	32.907293487619036	18.62497807296109	19.940398779747685
9	26.727826711190694	15.119013050702543	18.630422332994563	39.5227379051122
10	17.25674202568842	26.315354262025124	30.169323341935133	26.25858037035132
11	37.13138040543743	21.491955694549354	21.43356350679881	19.943100393214404
12	24.74091553095047	23.51348489661327	28.00199664201116	23.74360293042509
13	29.445082520164927	19.37567623981922	24.914565638496438	26.264675601519414
14	23.742667535102065	19.581537816261385	24.688805047679928	31.986989600956623
15	25.4340249766046	26.830185711058764	21.803502855852077	25.93228645648456
16	26.015933081461178	25.987801644126225	23.115269320701408	24.88099595371118
17	24.24231119034127	26.101923235924602	24.723271416846313	24.932494156887813
18	24.9824071105519	25.30401924430222	25.504346300653914	24.209227344491964
19	25.763398023411003	25.06441454337246	25.007560392414607	24.164627040801925
20	25.788561175735335	24.576940910832008	24.64931556559079	24.985182347841864
21	26.69771286300362	24.30806293910712	24.445250839154493	24.548973358734766
22	26.131873094727336	24.132647300762915	24.75463313924418	24.980846465265568
23	24.674400556356268	24.369815656591108	25.302115564872956	25.653668222179665
24	24.67099162157585	24.914421533920926	25.162589005636686	25.251997838866536
25	25.062821098212584	24.488586366536538	25.08318188736452	25.36541064788636
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	555.0
1	555.0
2	476.5
3	398.0
4	398.0
5	398.0
6	729.0
7	1060.0
8	1060.0
9	1060.0
10	1264.0
11	1468.0
12	1468.0
13	1468.0
14	2028.0
15	2588.0
16	2588.0
17	2588.0
18	4252.5
19	5917.0
20	5917.0
21	5917.0
22	9250.0
23	12583.0
24	12583.0
25	12583.0
26	18515.5
27	24448.0
28	24448.0
29	24448.0
30	30832.0
31	37216.0
32	37216.0
33	37216.0
34	47139.5
35	57063.0
36	57063.0
37	57063.0
38	67382.0
39	77701.0
40	77701.0
41	77701.0
42	90411.0
43	103121.0
44	103121.0
45	103121.0
46	114963.0
47	126805.0
48	126805.0
49	126805.0
50	131795.5
51	136786.0
52	136786.0
53	136786.0
54	129890.0
55	122994.0
56	122994.0
57	122994.0
58	113476.5
59	103959.0
60	103959.0
61	103959.0
62	90831.5
63	77704.0
64	77704.0
65	77704.0
66	63217.0
67	48730.0
68	48730.0
69	48730.0
70	37240.5
71	25751.0
72	25751.0
73	25751.0
74	19537.0
75	13323.0
76	13323.0
77	13323.0
78	10503.5
79	7684.0
80	7684.0
81	7684.0
82	5285.0
83	2886.0
84	2886.0
85	2886.0
86	2087.5
87	1289.0
88	1289.0
89	1289.0
90	866.0
91	443.0
92	443.0
93	443.0
94	272.5
95	102.0
96	102.0
97	102.0
98	249.5
99	397.0
100	397.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.063708809220007E-4
2	2.0141575131600017E-4
3	4.0283150263200034E-4
4	0.009466540311852008
5	0.03434138559937803
6	0.06344596166454006
7	0.10584397731655808
8	0.10523973006261009
9	0.1155119333797261
10	0.1327329801172441
11	0.1409910259212001
12	0.13152448560934812
13	0.1283018335882921
14	0.1157133491310421
15	0.1332365194955341
16	0.1206480350382841
17	0.12135299016789008
18	0.11007370809419409
19	0.09657885275602207
20	0.09174487472443807
21	0.09073779596785808
22	0.09939867327444608
23	0.08066700840205807
24	0.09053638021654208
25	0.08741443607114407
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	992971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.049604130526134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.22667421517995	44.82977866508306
2	13.685448506224226	15.888697362700333
3	4.164807055597812	7.252962025724255
4	1.7459267464201722	4.054014258823539
5	0.9132595495386185	2.6507177659569714
6	0.5420801021827425	1.8880521203246015
7	0.3302201711729148	1.34184051487516
8	0.23734094514077597	1.1022038327509571
9	0.16818814698643803	0.8786929816808522
>10	0.8571977522478397	8.815112795253215
>50	0.06818371865588818	2.8034140630096176
>100	0.05596569602976383	6.48350480804407
>500	0.004358698724861592	1.7053597572864756
>1k	3.4869589798892735E-4	0.3056490484869529
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1762	0.17744727690939616	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1258	0.12669050757776412	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0070787565800009E-4
2	0.0	0.0	0.0	0.0	1.0070787565800009E-4
3	0.0	0.0	0.0	0.0	1.0070787565800009E-4
4	0.0	0.0	0.0	0.0	1.0070787565800009E-4
5	0.0	0.0	0.0	0.0	1.0070787565800009E-4
6	0.0	0.0	0.0	0.0	1.0070787565800009E-4
7	0.0	0.0	0.0	0.0	1.0070787565800009E-4
8	0.0	0.0	0.0	0.0	1.0070787565800009E-4
9	0.0	0.0	0.0	1.0070787565800009E-4	2.0141575131600017E-4
10	0.0	0.0	0.0	1.0070787565800009E-4	2.0141575131600017E-4
11	0.0	0.0	0.0	1.0070787565800009E-4	2.0141575131600017E-4
12	0.0	0.0	0.0	1.0070787565800009E-4	7.049551296060006E-4
13	0.0	0.0	0.0	1.0070787565800009E-4	0.001007078756580001
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGTCG	70	4.4251465E-7	14.930394	9
AGGCGGT	45	6.7530223E-4	14.779581	11
CGAACGA	90	2.2846507E-9	14.777346	16
AACCGCG	65	3.3648848E-6	14.617169	7
CGCCGGT	145	0.0	13.760301	7
CGGTCCA	155	0.0	13.4855175	10
GGTATCA	465	0.0	13.481439	1
CTATTGT	50	0.00150092	13.297601	1
CGGTCGG	75	1.47545215E-5	12.668213	10
GTTACAC	60	4.0924526E-4	12.665658	3
CTTATAC	70	1.0909433E-4	12.213314	3
CGATAAC	110	3.8007784E-8	12.092385	10
AAACTAT	55	0.003065453	12.091776	6
TAAACTA	55	0.0030666108	12.091166	5
ATAATAC	55	0.0030689281	12.089947	3
GGTCGGC	95	1.0369713E-6	12.001465	11
GAACCGC	95	1.0376025E-6	12.00086	6
GTATCAA	1195	0.0	11.922535	1
ACGAGAC	120	1.0004442E-8	11.874055	19
TAACGAA	130	2.6102498E-9	11.691966	13
>>END_MODULE