FastQCFastQC Report
Thu 2 Feb 2017
SRR4062086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992971
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10040.1011107071606321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13400.024.9649371
ACGCTTA450.001398149924.44410917
GGTATCA6200.022.0085641
ACGTAGT500.002580321621.99969913
GTACGTA602.8725437E-421.99969911
CTAAGCG550.00448341419.9997253
TTAACGG550.00448341419.99972535
TCGACGC550.00448341419.99972514
ATAGCAC1051.7922648E-618.8568843
CTACACG951.596831E-518.5260624
GCGCAAT2150.018.41835235
CGCAATA2150.018.41835236
CAATACG2150.018.41835238
GCTATAC600.0074117818.3330823
TCGGGTA600.0074117818.33308216
TAAGCGT600.0074117818.3330824
CGCTTAC600.0074117818.33308218
TACGAAT2350.017.7869941
CGAGCCG2500.017.59975815
GCGGGTC2150.017.39511133
Produced by FastQC (version 0.11.5)