##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992971
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32686855910193	32.0	32.0	32.0	32.0	32.0
2	31.502052930045288	32.0	32.0	32.0	32.0	32.0
3	31.607627010255083	32.0	32.0	32.0	32.0	32.0
4	31.678911065882087	32.0	32.0	32.0	32.0	32.0
5	31.660210620451153	32.0	32.0	32.0	32.0	32.0
6	35.32227426581441	36.0	36.0	36.0	36.0	36.0
7	35.31848060013837	36.0	36.0	36.0	36.0	36.0
8	35.272596077831075	36.0	36.0	36.0	36.0	36.0
9	35.35503755900223	36.0	36.0	36.0	36.0	36.0
10	35.242774461691226	36.0	36.0	36.0	36.0	36.0
11	35.3549569927017	36.0	36.0	36.0	36.0	36.0
12	35.28125091266512	36.0	36.0	36.0	36.0	36.0
13	35.310317219737534	36.0	36.0	36.0	36.0	36.0
14	35.2793656612328	36.0	36.0	36.0	36.0	36.0
15	35.26265318926736	36.0	36.0	36.0	36.0	36.0
16	35.270431865583184	36.0	36.0	36.0	36.0	36.0
17	35.25259851496167	36.0	36.0	36.0	36.0	36.0
18	35.25551803627699	36.0	36.0	36.0	36.0	36.0
19	35.243688889202204	36.0	36.0	36.0	36.0	36.0
20	35.236558771605615	36.0	36.0	36.0	36.0	36.0
21	35.22690491464504	36.0	36.0	36.0	36.0	36.0
22	35.215420188505	36.0	36.0	36.0	36.0	36.0
23	35.18967422009303	36.0	36.0	36.0	36.0	36.0
24	35.17507661351641	36.0	36.0	36.0	36.0	36.0
25	35.16049914851491	36.0	36.0	36.0	36.0	36.0
26	35.124895893233536	36.0	36.0	36.0	36.0	36.0
27	35.111448370596925	36.0	36.0	36.0	36.0	36.0
28	35.094177977000335	36.0	36.0	36.0	36.0	36.0
29	35.0738772834252	36.0	36.0	36.0	36.0	36.0
30	35.05850624036351	36.0	36.0	36.0	36.0	36.0
31	35.0682990741925	36.0	36.0	36.0	36.0	36.0
32	35.03443504392374	36.0	36.0	36.0	36.0	36.0
33	35.010466569517135	36.0	36.0	36.0	36.0	36.0
34	34.99635135366491	36.0	36.0	36.0	36.0	36.0
35	34.985676318845165	36.0	36.0	36.0	36.0	36.0
36	34.972281164303894	36.0	36.0	36.0	36.0	36.0
37	34.955757016065924	36.0	36.0	36.0	36.0	36.0
38	34.942562270197215	36.0	36.0	36.0	36.0	36.0
39	34.92839871456467	36.0	36.0	36.0	36.0	36.0
40	34.92280036375685	36.0	36.0	36.0	36.0	36.0
41	34.89669788946505	36.0	36.0	36.0	36.0	36.0
42	34.86292046796936	36.0	36.0	36.0	32.0	36.0
43	34.85681354238945	36.0	36.0	36.0	32.0	36.0
44	34.80985950244267	36.0	36.0	36.0	32.0	36.0
45	34.805347789613194	36.0	36.0	36.0	32.0	36.0
46	34.78052027702722	36.0	36.0	36.0	32.0	36.0
47	34.74570153609723	36.0	36.0	36.0	32.0	36.0
48	34.72470192986502	36.0	36.0	36.0	32.0	36.0
49	34.716011847274494	36.0	36.0	36.0	32.0	36.0
50	34.31498301561677	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	12.0
21	24.0
22	59.0
23	154.0
24	374.0
25	931.0
26	2147.0
27	4039.0
28	6872.0
29	11073.0
30	16753.0
31	24835.0
32	38341.0
33	65733.0
34	153849.0
35	667770.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65789258949951	18.205143961823747	12.452211685986828	25.684751762689906
2	16.398970305645193	19.486139814445533	37.034955625652124	27.07993425425716
3	18.90376911193334	22.69352626373914	28.316478107004674	30.08622651732285
4	13.082255171601185	15.64013450543873	34.55609479028088	36.7215155326792
5	15.03821867730143	36.21851616866572	32.78447485825352	15.958790295779329
6	35.71225716789027	34.19871698037201	16.053858626141775	14.03516722559594
7	31.399406427780875	29.881537325863494	19.529170539723715	19.189885706631916
8	28.558739379095666	33.03258604732665	18.56207280977994	19.846601763797732
9	26.886787227421543	14.807381081622726	18.67849111404059	39.62734057691514
10	17.21028832693838	26.517719568567028	30.168081613744626	26.10391049074997
11	37.26020195957385	21.43295222116255	21.394481812661194	19.91236400660241
12	24.756110702125238	23.54237938469502	27.965972822972674	23.735537090207064
13	29.591095812465824	19.300563661980057	24.948160621005044	26.160179904549075
14	23.75588008108998	19.445280879300604	24.805054729695026	31.993784309914385
15	25.539215143241844	26.82505329964319	21.834978060789286	25.800753496325672
16	26.071609486298165	25.935653580323255	23.164267968083596	24.828468965294988
17	24.31732648788333	26.14829637522143	24.72539480004955	24.808982336845688
18	25.120270380504568	25.17999015076976	25.520684894120777	24.179054574604898
19	25.84858973726322	25.012714369301825	25.063873970136086	24.074821923298867
20	25.988450785926638	24.386989308819622	24.82627838963592	24.798281515617823
21	26.798869251972114	24.106544904131137	24.63757753247577	24.457008311420978
22	26.162194062062234	24.031718952517245	24.980689264842578	24.825397720577943
23	24.721189965457164	24.384321782128364	25.388581729558794	25.505906522855675
24	24.964550577865865	24.67894102908061	25.197791662134783	25.158716730918744
25	25.178354186640455	24.294136366932268	25.28641406369591	25.241095382731366
26	24.831867354974893	25.208718123279144	25.377706789557745	24.581707732188214
27	25.374181996160992	24.613128747610695	25.042247166569652	24.970442089658658
28	24.944357946088136	24.59580202104025	25.415477454141882	25.04436257872973
29	24.809862190371454	24.809056521686585	25.491256480597485	24.88982480734448
30	24.842087665515894	24.96203255733386	25.411397886714017	24.784481890436233
31	25.382136419025752	24.565797556824474	24.838313342799882	25.213752681349888
32	25.3415538560388	24.5378977241828	24.71494377428341	25.405604645494996
33	24.837784135827274	24.49305968879127	25.17576112801332	25.49339504736814
34	25.531792757426768	24.427923727191978	25.264410924083702	24.775872591297553
35	25.664723002709046	24.358238416064935	25.30670614419368	24.67033243703234
36	24.789797063151013	24.916185701668432	24.991515344386382	25.302501890794172
37	25.735906517791772	24.611993043015875	24.98592590429212	24.666174534900232
38	25.03688425945974	24.454893446032163	25.17394767823028	25.334274616277817
39	25.66684324274242	24.53699134508672	24.71605157095639	25.08011384121447
40	25.69208969849069	24.691456245952804	24.922983652090544	24.693470403465962
41	24.68619204724441	25.02064526004971	25.401122296478018	24.89204039622786
42	25.787270727668986	24.939068851191028	24.861016553129947	24.41264386801004
43	25.439226377451185	24.270876660096942	25.067300206759928	25.22259675569194
44	25.02744413984527	24.77999367525878	24.89812936714317	25.29443281775278
45	25.210735686590464	24.799033183946825	24.944559141950755	25.04567198751196
46	25.02943258804347	24.69265731202169	24.919255509105724	25.358654590829115
47	25.173823018759993	24.729194613299175	25.28339395990187	24.81358840803896
48	25.771219035169256	25.047611086660737	24.378789877747018	24.802380000422986
49	25.159274157000105	25.251122422054863	24.43345129927025	25.15615212167478
50	25.1980692091633	25.157382817653463	24.61496473678221	25.029583236401027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	245.0
1	143.0
2	41.0
3	125.0
4	209.0
5	209.5
6	210.0
7	155.0
8	100.0
9	153.0
10	206.0
11	357.5
12	509.0
13	963.0
14	1417.0
15	1783.5
16	2150.0
17	2122.5
18	2095.0
19	2049.5
20	2004.0
21	2252.0
22	2500.0
23	3039.5
24	3579.0
25	4471.5
26	5364.0
27	6680.0
28	7996.0
29	9582.0
30	11168.0
31	12809.0
32	14450.0
33	16478.5
34	18507.0
35	21395.0
36	24283.0
37	28616.5
38	32950.0
39	36946.5
40	40943.0
41	45023.0
42	49103.0
43	51710.0
44	54317.0
45	59849.5
46	65382.0
47	69535.0
48	73688.0
49	76314.0
50	78940.0
51	77282.5
52	75625.0
53	75119.5
54	74614.0
55	74784.5
56	74955.0
57	72242.0
58	69529.0
59	64527.0
60	59525.0
61	52468.5
62	45412.0
63	38562.5
64	31713.0
65	26942.0
66	22171.0
67	19148.5
68	16126.0
69	14053.0
70	11980.0
71	9327.0
72	6674.0
73	5792.0
74	4910.0
75	3726.0
76	2542.0
77	2219.0
78	1896.0
79	1576.5
80	1257.0
81	976.0
82	695.0
83	612.5
84	530.0
85	404.5
86	279.0
87	190.0
88	101.0
89	64.0
90	27.0
91	21.0
92	15.0
93	13.0
94	11.0
95	14.5
96	18.0
97	13.0
98	8.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03142085720529603
2	0.005538933161190004
3	5.035393782900005E-4
4	0.0
5	1.0070787565800009E-4
6	1.0070787565800009E-4
7	0.0
8	0.0
9	0.0
10	1.0070787565800009E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0141575131600017E-4
17	0.0
18	0.0
19	0.0
20	3.0212362697400023E-4
21	0.0
22	0.0
23	1.0070787565800009E-4
24	7.049551296060006E-4
25	3.0212362697400023E-4
26	5.035393782900005E-4
27	5.035393782900005E-4
28	0.0017120338861860015
29	7.049551296060006E-4
30	0.0015106181348700013
31	1.0070787565800009E-4
32	9.063708809220007E-4
33	4.0283150263200034E-4
34	9.063708809220007E-4
35	1.0070787565800009E-4
36	2.0141575131600017E-4
37	0.001208494507896001
38	0.0
39	9.063708809220007E-4
40	0.0
41	7.049551296060006E-4
42	0.004733270155926004
43	0.0032226520210560028
44	0.004531854404610004
45	0.0021148653888180017
46	0.002416989015792002
47	7.049551296060006E-4
48	0.0030212362697400025
49	0.002920528394082002
50	0.001007078756580001
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	992971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.56466883878145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.08045196731811	45.91271595376904
2	14.026895252012952	16.71014741044855
3	4.2090305844585805	7.521285386867343
4	1.713556259366018	4.082696445030319
5	0.8570872503632276	2.5526059116913715
6	0.49730251315194063	1.7772995705153463
7	0.3127825345302082	1.3041551661492603
8	0.2312319230310464	1.1018602336239105
9	0.16776926524226562	0.8993808652933116
>10	0.7885887489751553	8.312034425461803
>50	0.06361217553820359	2.6177445930978513
>100	0.04778065437342321	5.601632139793847
>500	0.0037408337414679147	1.5047542577656188
>1k	1.7003789733945068E-4	0.10168764049247367
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1004	0.1011107071606321	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0141575131600017E-4
2	0.0	0.0	0.0	0.0	2.0141575131600017E-4
3	0.0	0.0	0.0	0.0	2.0141575131600017E-4
4	0.0	0.0	0.0	0.0	2.0141575131600017E-4
5	0.0	0.0	0.0	0.0	3.0212362697400023E-4
6	0.0	0.0	0.0	0.0	3.0212362697400023E-4
7	0.0	0.0	0.0	0.0	3.0212362697400023E-4
8	0.0	0.0	0.0	0.0	3.0212362697400023E-4
9	0.0	0.0	0.0	0.0	4.0283150263200034E-4
10	0.0	0.0	0.0	0.0	4.0283150263200034E-4
11	0.0	0.0	0.0	0.0	4.0283150263200034E-4
12	0.0	0.0	0.0	0.0	9.063708809220007E-4
13	0.0	0.0	0.0	0.0	9.063708809220007E-4
14	0.0	0.0	0.0	0.0	9.063708809220007E-4
15	0.0	0.0	0.0	0.0	9.063708809220007E-4
16	0.0	0.0	0.0	0.0	0.001007078756580001
17	0.0	0.0	0.0	0.0	0.001007078756580001
18	0.0	0.0	0.0	0.0	0.001007078756580001
19	0.0	0.0	0.0	1.0070787565800009E-4	0.001007078756580001
20	0.0	0.0	0.0	2.0141575131600017E-4	0.0011077866322380008
21	0.0	0.0	0.0	2.0141575131600017E-4	0.001208494507896001
22	0.0	0.0	0.0	2.0141575131600017E-4	0.001208494507896001
23	0.0	0.0	0.0	3.0212362697400023E-4	0.001309202383554001
24	0.0	0.0	0.0	5.035393782900005E-4	0.001309202383554001
25	0.0	0.0	0.0	8.056630052640007E-4	0.001309202383554001
26	0.0	0.0	0.0	0.0015106181348700013	0.001309202383554001
27	0.0	0.0	0.0	0.0016113260105280014	0.001309202383554001
28	0.0	0.0	0.0	0.0028198205184240023	0.001309202383554001
29	0.0	0.0	0.0	0.0053375174098740045	0.001309202383554001
30	0.0	0.0	0.0	0.009265124560536008	0.001309202383554001
31	0.0	0.0	0.0	0.016213967980938014	0.001309202383554001
32	0.0	0.0	0.0	0.02316281140134002	0.001309202383554001
33	0.0	0.0	0.0	0.028802452438188023	0.0014099102592120012
34	0.0	0.0	0.0	0.03645625098819603	0.0017120338861860015
35	0.0	0.0	0.0	0.04320367865728204	0.0017120338861860015
36	0.0	0.0	0.0	0.05438225285532004	0.0017120338861860015
37	0.0	0.0	0.0	0.07099905233889006	0.0017120338861860015
38	0.0	0.0	0.0	0.09355761648628208	0.0019134496375020015
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1340	0.0	24.964937	1
ACGCTTA	45	0.0013981499	24.444109	17
GGTATCA	620	0.0	22.008564	1
ACGTAGT	50	0.0025803216	21.999699	13
GTACGTA	60	2.8725437E-4	21.999699	11
CTAAGCG	55	0.004483414	19.999725	3
TTAACGG	55	0.004483414	19.999725	35
TCGACGC	55	0.004483414	19.999725	14
ATAGCAC	105	1.7922648E-6	18.856884	3
CTACACG	95	1.596831E-5	18.526062	4
GCGCAAT	215	0.0	18.418352	35
CGCAATA	215	0.0	18.418352	36
CAATACG	215	0.0	18.418352	38
GCTATAC	60	0.00741178	18.333082	3
TCGGGTA	60	0.00741178	18.333082	16
TAAGCGT	60	0.00741178	18.333082	4
CGCTTAC	60	0.00741178	18.333082	18
TACGAAT	235	0.0	17.78699	41
CGAGCCG	250	0.0	17.599758	15
GCGGGTC	215	0.0	17.395111	33
>>END_MODULE