FastQCFastQC Report
Thu 2 Feb 2017
SRR4062085_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062085_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337168
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC33340.14265127710117545No Hit
GGGTAGGCACACGCTGAGCCAGTCA32720.13999849390373306No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC29680.12699129887111238No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG26870.11496820083109131No Hit
GAATAGGACCGCGGTTCTATTTTGT26410.11300000684589212No Hit
TCGTAGTTCCGACCATAAACGATGC25750.11017607634538895No Hit
GATTAAGAGGGACGGCCGGGGGCAT24200.10354411835178301No Hit
GTCTTGCGCCGGTCCAAGAATTTCA23700.10140477706352302No Hit
GTATCAACGCAGAGTACTTTTTTTT23510.10059182737398424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20450.013.6054031
CGAACGA1400.013.57112316
CGACTAT500.001499953113.29998614
TCGACGC655.454835E-513.153831514
GCGTTAT2750.013.12163451
AATCGCT1850.012.3243115
GGTATCA7250.012.3119521
ACGGTAT3550.012.3116919
CGGTCCA4900.012.02168210
GTCCTAG3250.011.9794691
CGTCGTA4450.011.95632310
GTAGGAC6000.011.8737153
CGTTATT2800.011.8724432
CGACCAT4350.011.794353510
TCGCGTA2100.011.7636579
CGCCGGT4450.011.743327
GTCCTAA2850.011.6616571
GGGTAGG8200.011.5803921
CAAATCG1900.011.50023313
TCGCCAG3800.011.49875517
Produced by FastQC (version 0.11.5)