##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062085_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2337168
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.197995180491947	32.0	32.0	32.0	32.0	32.0
2	30.679071851060773	32.0	32.0	32.0	32.0	32.0
3	30.714727396575686	32.0	32.0	32.0	32.0	32.0
4	30.716723829865888	32.0	32.0	32.0	32.0	32.0
5	30.610360915432693	32.0	32.0	32.0	32.0	32.0
6	34.25954873590602	36.0	36.0	36.0	32.0	36.0
7	34.18948359724247	36.0	36.0	36.0	32.0	36.0
8	34.13699229152547	36.0	36.0	36.0	32.0	36.0
9	34.29367721960937	36.0	36.0	36.0	32.0	36.0
10	33.93879558508417	36.0	36.0	36.0	32.0	36.0
11	34.27699164116572	36.0	36.0	36.0	32.0	36.0
12	34.06444637270406	36.0	36.0	36.0	32.0	36.0
13	34.17515386142545	36.0	36.0	36.0	32.0	36.0
14	34.06387388497532	36.0	36.0	36.0	32.0	36.0
15	34.000147186680636	36.0	36.0	36.0	32.0	36.0
16	34.01006388928823	36.0	36.0	36.0	32.0	36.0
17	33.930774338857965	36.0	36.0	36.0	32.0	36.0
18	33.9445341541558	36.0	36.0	36.0	32.0	36.0
19	33.95242661203645	36.0	36.0	36.0	32.0	36.0
20	33.93513688361299	36.0	36.0	36.0	32.0	36.0
21	33.9077973855538	36.0	36.0	36.0	32.0	36.0
22	33.87496020825204	36.0	36.0	36.0	32.0	36.0
23	33.844877218924786	36.0	36.0	36.0	32.0	36.0
24	33.81923464637544	36.0	36.0	36.0	32.0	36.0
25	33.44446184442025	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	47.0
5	194.0
6	514.0
7	121.0
8	426.0
9	314.0
10	159.0
11	44.0
12	91.0
13	114.0
14	316.0
15	466.0
16	739.0
17	1122.0
18	1586.0
19	2277.0
20	3588.0
21	5029.0
22	7722.0
23	11507.0
24	16403.0
25	23252.0
26	31405.0
27	39500.0
28	51783.0
29	67523.0
30	87612.0
31	117761.0
32	164309.0
33	226856.0
34	481446.0
35	992939.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.4540589618837	16.684216629149255	10.750033053261319	26.11169135570572
2	17.032159958171476	18.967670605491023	37.108740048289356	26.89142938804815
3	19.683170214714135	22.471732714232783	27.700955948987527	30.144141122065555
4	12.472971775965602	14.999129191594538	35.397013747647	37.13088528479286
5	14.691172284796487	36.55468928899323	33.69426901535953	15.059869410850757
6	35.670464242149706	35.14537725213895	16.006119164433493	13.17803934127786
7	31.334369631023478	29.656860960347263	19.927756144218716	19.081013264410544
8	28.437727695822158	33.5081723122737	18.512342318300608	19.54175767360354
9	26.792786656299466	14.035175653358522	18.01588066581416	41.15615702452785
10	15.73594556508021	26.809652163741788	31.509381334505598	25.945020936672403
11	38.19938650701052	20.730518555399257	21.609195796300444	19.460899141289776
12	24.497728525969098	23.479324937541428	28.67832902803705	23.34461750845242
13	29.722512366725134	19.206618779256633	24.816137880095248	26.254730973922985
14	23.22934319401916	19.489176606459594	24.707589312487897	32.57389088703334
15	25.10334314207711	27.619268783001683	21.560412892699652	25.716975182221553
16	25.99187334934102	25.80573577889684	23.47835213629811	24.724038735464028
17	24.132976146053213	26.280126966282992	24.75435520141481	24.83254168624899
18	24.962432361115393	25.195762952910655	25.751438450756837	24.09036623521711
19	25.706643595503692	25.034553206798854	24.688228781418577	24.570574416278877
20	25.753333838703753	24.616336055909386	24.49144960364826	25.138880501738605
21	26.52867102678298	24.457879880237417	24.15663184655109	24.856817246428513
22	25.959433499594166	24.46518481079889	24.812683509766842	24.762698179840108
23	24.314773569626627	24.743092279514574	25.350369586545256	25.59176456431354
24	24.790541284234717	24.884290735507058	25.102882420885784	25.222285559372438
25	24.84454147873173	24.79546283813299	24.879316222332235	25.480679460803042
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	124.0
1	124.0
2	349.0
3	574.0
4	574.0
5	574.0
6	1287.5
7	2001.0
8	2001.0
9	2001.0
10	2578.5
11	3156.0
12	3156.0
13	3156.0
14	4567.0
15	5978.0
16	5978.0
17	5978.0
18	10083.0
19	14188.0
20	14188.0
21	14188.0
22	22404.0
23	30620.0
24	30620.0
25	30620.0
26	45412.0
27	60204.0
28	60204.0
29	60204.0
30	76707.5
31	93211.0
32	93211.0
33	93211.0
34	116931.5
35	140652.0
36	140652.0
37	140652.0
38	164033.0
39	187414.0
40	187414.0
41	187414.0
42	214866.5
43	242319.0
44	242319.0
45	242319.0
46	268579.0
47	294839.0
48	294839.0
49	294839.0
50	305881.0
51	316923.0
52	316923.0
53	316923.0
54	298893.5
55	280864.0
56	280864.0
57	280864.0
58	259343.0
59	237822.0
60	237822.0
61	237822.0
62	210988.5
63	184155.0
64	184155.0
65	184155.0
66	152258.5
67	120362.0
68	120362.0
69	120362.0
70	90038.5
71	59715.0
72	59715.0
73	59715.0
74	45572.0
75	31429.0
76	31429.0
77	31429.0
78	24806.0
79	18183.0
80	18183.0
81	18183.0
82	12546.5
83	6910.0
84	6910.0
85	6910.0
86	5046.5
87	3183.0
88	3183.0
89	3183.0
90	2130.5
91	1078.0
92	1078.0
93	1078.0
94	659.0
95	240.0
96	240.0
97	240.0
98	632.0
99	1024.0
100	1024.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013263915987211873
2	2.9950778035639714E-4
3	5.990155607127943E-4
4	0.011081787873186694
5	0.035641425862411265
6	0.06653351406488536
7	0.11017607634538895
8	0.11291443319436173
9	0.12335441868107043
10	0.1379875130927687
11	0.14200947471469744
12	0.13781636578970788
13	0.13186899700834515
14	0.11894737562725487
15	0.13674669514557788
16	0.12292655042341843
17	0.12857441142442477
18	0.11603787147522131
19	0.10110526928316663
20	0.09614199749440348
21	0.0947728190699171
22	0.10623968837499059
23	0.0851885700985124
24	0.0950295400245083
25	0.09156380713752713
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2337168.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.39855443161944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78634566981314	36.449388128805516
2	14.606436900308548	14.430737365438128
3	5.151589266610268	7.634431883879814
4	2.2628898672376856	4.471339531180036
5	1.1991930809059552	2.961920234057712
6	0.7160566180929142	2.122329709499049
7	0.45769077359622284	1.5826483814640115
8	0.29659696889833165	1.1721169209902058
9	0.22480737332971368	0.9994643341251527
>10	1.1236464074044603	9.973220384721026
>50	0.08650671418824908	3.0020899058724817
>100	0.07219571731772538	7.226251456964977
>500	0.011014430117355057	3.735589554190181
>1k	0.005030212179579352	4.2384722088118005
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	3334	0.14265127710117545	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	3272	0.13999849390373306	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2968	0.12699129887111238	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2687	0.11496820083109131	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2641	0.11300000684589212	No Hit
TCGTAGTTCCGACCATAAACGATGC	2575	0.11017607634538895	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2420	0.10354411835178301	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2370	0.10140477706352302	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2351	0.10059182737398424	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2836047729559877E-4
2	0.0	0.0	0.0	0.0	1.2836047729559877E-4
3	0.0	0.0	0.0	0.0	1.2836047729559877E-4
4	0.0	0.0	0.0	0.0	1.2836047729559877E-4
5	0.0	0.0	0.0	0.0	1.2836047729559877E-4
6	0.0	0.0	0.0	0.0	1.2836047729559877E-4
7	0.0	0.0	0.0	0.0	1.2836047729559877E-4
8	0.0	0.0	0.0	0.0	1.2836047729559877E-4
9	0.0	0.0	0.0	4.278682576519959E-5	1.2836047729559877E-4
10	0.0	0.0	0.0	4.278682576519959E-5	1.2836047729559877E-4
11	0.0	0.0	0.0	4.278682576519959E-5	1.2836047729559877E-4
12	0.0	4.278682576519959E-5	0.0	4.278682576519959E-5	3.8508143188679635E-4
13	0.0	4.278682576519959E-5	0.0	4.278682576519959E-5	5.134419091823951E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2045	0.0	13.605403	1
CGAACGA	140	0.0	13.571123	16
CGACTAT	50	0.0014999531	13.299986	14
TCGACGC	65	5.454835E-5	13.1538315	14
GCGTTAT	275	0.0	13.1216345	1
AATCGCT	185	0.0	12.32431	15
GGTATCA	725	0.0	12.311952	1
ACGGTAT	355	0.0	12.311691	9
CGGTCCA	490	0.0	12.021682	10
GTCCTAG	325	0.0	11.979469	1
CGTCGTA	445	0.0	11.956323	10
GTAGGAC	600	0.0	11.873715	3
CGTTATT	280	0.0	11.872443	2
CGACCAT	435	0.0	11.7943535	10
TCGCGTA	210	0.0	11.763657	9
CGCCGGT	445	0.0	11.74332	7
GTCCTAA	285	0.0	11.661657	1
GGGTAGG	820	0.0	11.580392	1
CAAATCG	190	0.0	11.500233	13
TCGCCAG	380	0.0	11.498755	17
>>END_MODULE