FastQCFastQC Report
Thu 2 Feb 2017
SRR4062085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337168
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT35620.15240667337564096No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC34500.14761454888993858No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA28600.12237032168847084No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT27750.11873344149842886No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA27560.11792049180889008No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA26910.11513934813415211No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT26100.11167361524717094No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC25060.10722378536759018No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG23620.10106248245740145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22250.029.670831
GGTATCA9250.022.1248151
CAACGCA30900.020.8597075
TCAACGC30950.020.8260064
AACGCAG31150.020.7629156
ATCAACG31450.020.5648563
CGGATCG2050.020.3904426
TACGAAT5150.019.65109641
CGATTTT2400.019.25018931
TAGGACG4700.019.1904434
CGAATGC5400.019.14956343
CGAGCCG8750.018.60549715
TCGATTT2250.018.57756230
GTAGGAC8650.018.5654623
CGCAATA5350.018.50485236
ATACGAA5800.018.20746240
TATCAAC36100.018.1608412
CGGACCA11550.018.0942529
TGTAGGA9450.017.92612
TCTAGCG5650.017.91167828
Produced by FastQC (version 0.11.5)