##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062085_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2337168 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.261273044984357 32.0 32.0 32.0 32.0 32.0 2 31.39043663099957 32.0 32.0 32.0 32.0 32.0 3 31.545743823293833 32.0 32.0 32.0 32.0 32.0 4 31.631543389264273 32.0 32.0 32.0 32.0 32.0 5 31.598465322133453 32.0 32.0 32.0 32.0 32.0 6 35.23444998391216 36.0 36.0 36.0 36.0 36.0 7 35.24304628507664 36.0 36.0 36.0 36.0 36.0 8 35.18787010604287 36.0 36.0 36.0 36.0 36.0 9 35.284807938496506 36.0 36.0 36.0 36.0 36.0 10 35.15307029704326 36.0 36.0 36.0 36.0 36.0 11 35.28851926776338 36.0 36.0 36.0 36.0 36.0 12 35.2012076153704 36.0 36.0 36.0 36.0 36.0 13 35.24548812922306 36.0 36.0 36.0 36.0 36.0 14 35.20001899735064 36.0 36.0 36.0 36.0 36.0 15 35.17854172228954 36.0 36.0 36.0 36.0 36.0 16 35.19320177240147 36.0 36.0 36.0 36.0 36.0 17 35.16661018805666 36.0 36.0 36.0 36.0 36.0 18 35.16883681446948 36.0 36.0 36.0 36.0 36.0 19 35.16384787058526 36.0 36.0 36.0 36.0 36.0 20 35.1614094493849 36.0 36.0 36.0 36.0 36.0 21 35.152754530269114 36.0 36.0 36.0 36.0 36.0 22 35.13373193540216 36.0 36.0 36.0 36.0 36.0 23 35.10401905211778 36.0 36.0 36.0 36.0 36.0 24 35.08637504877698 36.0 36.0 36.0 36.0 36.0 25 35.06838104920143 36.0 36.0 36.0 36.0 36.0 26 35.02748112245247 36.0 36.0 36.0 36.0 36.0 27 35.008359690018004 36.0 36.0 36.0 36.0 36.0 28 34.99437267667536 36.0 36.0 36.0 36.0 36.0 29 34.96801941495006 36.0 36.0 36.0 36.0 36.0 30 34.945061715717486 36.0 36.0 36.0 36.0 36.0 31 34.9502153888809 36.0 36.0 36.0 36.0 36.0 32 34.924062369500184 36.0 36.0 36.0 32.0 36.0 33 34.91008049057663 36.0 36.0 36.0 32.0 36.0 34 34.8973582558036 36.0 36.0 36.0 32.0 36.0 35 34.88466254886256 36.0 36.0 36.0 32.0 36.0 36 34.8554515550444 36.0 36.0 36.0 32.0 36.0 37 34.84447074408001 36.0 36.0 36.0 32.0 36.0 38 34.82385305634854 36.0 36.0 36.0 32.0 36.0 39 34.804812491014765 36.0 36.0 36.0 32.0 36.0 40 34.80258115805111 36.0 36.0 36.0 32.0 36.0 41 34.774539956049374 36.0 36.0 36.0 32.0 36.0 42 34.74828510402333 36.0 36.0 36.0 32.0 36.0 43 34.74778449816188 36.0 36.0 36.0 32.0 36.0 44 34.69331772469929 36.0 36.0 36.0 32.0 36.0 45 34.685755153245296 36.0 36.0 36.0 32.0 36.0 46 34.663772993640166 36.0 36.0 36.0 32.0 36.0 47 34.644223692948046 36.0 36.0 36.0 32.0 36.0 48 34.62370655425712 36.0 36.0 36.0 32.0 36.0 49 34.60280390626605 36.0 36.0 36.0 32.0 36.0 50 34.18847682323222 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 4.0 20 19.0 21 44.0 22 128.0 23 409.0 24 1018.0 25 2521.0 26 5609.0 27 10666.0 28 18086.0 29 29064.0 30 43825.0 31 65233.0 32 100753.0 33 174796.0 34 400923.0 35 1484069.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.140196675957185 17.145202599519266 11.138950331103151 26.575650393420396 2 16.446014112464127 19.460797926303858 36.81157237462255 27.28161558660946 3 18.98832045518434 22.881887142659714 28.05931807066944 30.07047433148651 4 12.201058889270168 15.357402940142034 35.6471470148034 36.7943911557844 5 14.382583700694045 36.93420281905401 33.44493568495533 15.238277795296614 6 35.88259103768499 35.2182388570079 15.873426083920512 13.025744021386604 7 31.454692174460714 29.6362948662655 19.912817563820827 18.996195395452958 8 28.437664729279195 33.68914857639673 18.386311980995803 19.486874713328266 9 26.840903178547716 13.7122791344054 17.974146488399636 41.472671198647255 10 15.705467345722408 26.877711348825308 31.459454972622837 25.957366332829444 11 38.41871872283036 20.619698712287693 21.694931643767156 19.266650921114785 12 24.583106206405535 23.36291046506752 28.673401889296695 23.380581439230248 13 29.81531494526709 19.047368439068137 24.890209005086497 26.247107610578276 14 23.321772333011577 19.352866375031663 24.688426334777816 32.636934957178944 15 25.129601295242786 27.538242864868934 21.537347764473928 25.79480807541435 16 26.122265256629017 25.722205012262812 23.425602486449296 24.729927244658874 17 24.280240016977814 26.23559795444743 24.697283207711212 24.786878820863542 18 25.17345808852096 25.000427868989938 25.784112710960805 24.042001331528297 19 25.82527229535917 24.922513058539224 24.68714273000486 24.565071916096745 20 25.907479978897456 24.442838506066554 24.525032314874597 25.124649200161393 21 26.78667240581114 24.333016995797475 24.1252867789451 24.755023819446286 22 26.02863807822116 24.442616020756745 24.88438999678243 24.644355904239664 23 24.413948847494062 24.515139690428757 25.447421837026692 25.62348962505049 24 24.99964700581777 24.764231976651896 25.10062473552178 25.135496282008553 25 24.95719167092397 24.577714339014584 25.03934258729177 25.425751402769688 26 24.766243773952713 25.588482074588914 25.223423158991885 24.42185099246649 27 25.335064779475946 24.89692618391552 24.81712848072019 24.950880555888343 28 24.75963050423789 24.71243588827498 25.36567269870623 25.162260908780894 29 24.63607571918421 24.967374961598725 25.348948853352915 25.047600465864157 30 24.508458215525327 25.18137583777752 25.531890313614614 24.77827563308254 31 25.362277293809584 24.94976809461055 24.504227352855604 25.183727258724264 32 25.16068716171405 24.82052927711101 24.46342767901076 25.55535588216418 33 24.554041894453906 24.747695813592486 25.113952452528903 25.5843098394247 34 25.357860610751626 24.88497757742005 25.26565440944602 24.4915074023823 35 25.82599144859939 24.513073767703634 25.39619649651864 24.264738287178332 36 24.46762446484332 25.312664996838052 24.99762532913794 25.22208520918069 37 25.601689595815717 24.93022415983385 24.773065391825092 24.695020852525342 38 24.7926656503365 24.706279012154457 25.203633287651243 25.297422049857797 39 25.693173309372526 24.608518922619428 24.5439103181225 25.154397449885547 40 25.75954200922913 24.75186818217798 25.077690278606774 24.410899529986114 41 24.772457323981747 25.252744375186126 25.386283429690256 24.58851487114187 42 25.836108559220243 25.212458286033613 25.03205968900069 23.919373465745448 43 25.30051710379088 24.402977251284202 25.268297066179446 25.02820857874547 44 24.648790105876433 25.185489143643476 24.89238634457593 25.27333440590416 45 24.939155884165125 25.35672109262682 24.78524851098788 24.918874512220167 46 24.7485150514437 25.058597811492618 24.900582683458858 25.292304453604824 47 25.090591685974495 24.712949829599058 25.451674806975205 24.744783677451238 48 25.801109936801208 25.420804682786567 24.149084109590213 24.629001270822013 49 24.891850837594404 25.472711324105173 24.567467533856785 25.067970304443637 50 25.09260285366973 25.59766654072878 24.475093608359995 24.834636997241493 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7.0 1 10.0 2 13.0 3 78.5 4 144.0 5 168.5 6 193.0 7 156.5 8 120.0 9 182.5 10 245.0 11 379.5 12 514.0 13 1004.5 14 1495.0 15 2012.5 16 2530.0 17 2793.0 18 3056.0 19 3340.0 20 3624.0 21 4739.5 22 5855.0 23 7165.0 24 8475.0 25 11261.0 26 14047.0 27 17499.5 28 20952.0 29 25015.0 30 29078.0 31 33395.0 32 37712.0 33 44271.0 34 50830.0 35 58297.5 36 65765.0 37 76535.5 38 87306.0 39 94009.5 40 100713.0 41 110174.0 42 119635.0 43 123052.5 44 126470.0 45 136773.0 46 147076.0 47 156780.0 48 166484.0 49 175019.0 50 183554.0 51 177877.5 52 172201.0 53 171286.0 54 170371.0 55 172697.0 56 175023.0 57 169377.5 58 163732.0 59 150347.5 60 136963.0 61 120077.5 62 103192.0 63 88314.0 64 73436.0 65 62284.5 66 51133.0 67 44455.0 68 37777.0 69 34114.5 70 30452.0 71 23427.0 72 16402.0 73 14570.0 74 12738.0 75 9329.5 76 5921.0 77 5195.0 78 4469.0 79 3830.0 80 3191.0 81 2568.0 82 1945.0 83 1577.5 84 1210.0 85 933.0 86 656.0 87 449.0 88 242.0 89 166.5 90 91.0 91 69.0 92 47.0 93 40.5 94 34.0 95 28.5 96 23.0 97 23.0 98 23.0 99 13.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0314911037631869 2 0.003636880190041965 3 2.1393412882599798E-4 4 8.557365153039919E-5 5 4.278682576519959E-5 6 1.7114730306079837E-4 7 0.0 8 0.0 9 0.0 10 4.278682576519959E-5 11 0.0 12 8.557365153039919E-5 13 0.0 14 0.0 15 0.0 16 8.557365153039919E-4 17 0.0 18 1.7114730306079837E-4 19 0.0 20 3.850814318867963E-4 21 8.557365153039919E-5 22 0.0 23 0.0 24 8.129496895387922E-4 25 2.5672095459119753E-4 26 4.7065508341719557E-4 27 3.4229460612159675E-4 28 0.0015831125533123849 29 2.5672095459119753E-4 30 0.001369178424486387 31 3.4229460612159675E-4 32 7.701628637735926E-4 33 3.850814318867963E-4 34 6.418023864779939E-4 35 2.9950778035639714E-4 36 8.557365153039919E-5 37 0.0017542598563731833 38 4.278682576519959E-5 39 7.701628637735926E-4 40 1.2836047729559877E-4 41 0.0010268838183647901 42 0.00517720591758915 43 0.004834911311467554 44 0.004449829879580758 45 0.0020537676367295803 46 0.0021393412882599796 47 0.0014119652502515866 48 0.00419310892498956 49 0.005048845440293552 50 0.0015831125533123849 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2337168.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.50202261604151 #Duplication Level Percentage of deduplicated Percentage of total 1 72.20563557173409 35.743250050776325 2 15.688193740342523 15.53194642678553 3 5.387918263512207 8.00138555201293 4 2.3706671606962075 4.694112776155624 5 1.2607801677901431 3.1205584189902136 6 0.7615581891530855 2.261920242173259 7 0.4694830201049062 1.6268251355356378 8 0.3197011067648276 1.2660681133956797 9 0.24087305634188208 1.07313331343678 >10 1.134481565265472 9.915889216718673 >50 0.08055915971417534 2.7910583909366444 >100 0.06598609104552376 6.714877540339048 >500 0.009384012354505174 3.245370405947263 >1k 0.004778895180534821 4.013604416796584 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 3562 0.15240667337564096 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC 3450 0.14761454888993858 No Hit GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA 2860 0.12237032168847084 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT 2775 0.11873344149842886 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA 2756 0.11792049180889008 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA 2691 0.11513934813415211 No Hit TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT 2610 0.11167361524717094 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC 2506 0.10722378536759018 No Hit ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG 2362 0.10106248245740145 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.7114730306079837E-4 2 0.0 0.0 0.0 0.0 1.7114730306079837E-4 3 0.0 0.0 0.0 0.0 1.7114730306079837E-4 4 0.0 0.0 0.0 0.0 1.7114730306079837E-4 5 0.0 0.0 0.0 0.0 2.1393412882599795E-4 6 0.0 0.0 0.0 0.0 3.4229460612159675E-4 7 0.0 0.0 0.0 0.0 3.4229460612159675E-4 8 0.0 0.0 0.0 0.0 3.4229460612159675E-4 9 0.0 0.0 0.0 0.0 3.4229460612159675E-4 10 0.0 0.0 0.0 0.0 3.4229460612159675E-4 11 0.0 0.0 0.0 0.0 3.4229460612159675E-4 12 0.0 0.0 0.0 0.0 7.701628637735927E-4 13 0.0 0.0 0.0 0.0 9.41310166834391E-4 14 0.0 0.0 0.0 4.278682576519959E-5 9.840969925995907E-4 15 0.0 0.0 0.0 1.2836047729559877E-4 0.0011980311214255886 16 0.0 0.0 0.0 2.1393412882599795E-4 0.0013263915987211873 17 0.0 0.0 0.0 2.5672095459119753E-4 0.0013263915987211873 18 0.0 0.0 0.0 4.278682576519959E-4 0.001454752076016786 19 0.0 0.0 0.0 5.134419091823951E-4 0.001454752076016786 20 4.278682576519959E-5 0.0 0.0 5.990155607127943E-4 0.0016686862048427842 21 4.278682576519959E-5 0.0 0.0 6.845892122431935E-4 0.0017542598563731833 22 4.278682576519959E-5 0.0 0.0 8.129496895387923E-4 0.0017542598563731833 23 4.278682576519959E-5 0.0 0.0 0.0012408179471907882 0.0017542598563731833 24 4.278682576519959E-5 0.0 0.0 0.0016258993790775845 0.0017542598563731833 25 4.278682576519959E-5 0.0 0.0 0.0021821281140251792 0.0017542598563731833 26 4.278682576519959E-5 0.0 0.0 0.002738356848972774 0.001797046682138383 27 4.278682576519959E-5 0.0 0.0 0.00312343828085957 0.001797046682138383 28 4.278682576519959E-5 0.0 0.0 0.003594093364276766 0.001797046682138383 29 4.278682576519959E-5 0.0 0.0 0.004407043053815558 0.001797046682138383 30 4.278682576519959E-5 0.0 0.0 0.005605074175241147 0.001797046682138383 31 4.278682576519959E-5 0.0 0.0 0.00731654720584913 0.0018398335079035824 32 4.278682576519959E-5 0.0 0.0 0.009113593887987513 0.001882620333668782 33 4.278682576519959E-5 0.0 0.0 0.011723590259664688 0.001882620333668782 34 4.278682576519959E-5 0.0 0.0 0.01467588123746346 0.0019681939851991814 35 4.278682576519959E-5 0.0 0.0 0.018056040472914228 0.0019681939851991814 36 4.278682576519959E-5 0.0 0.0 0.02194964161754739 0.0019681939851991814 37 4.278682576519959E-5 0.0 0.0 0.02721242118666694 0.0019681939851991814 38 4.278682576519959E-5 4.278682576519959E-5 0.0 0.03418667378639447 0.0019681939851991814 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2225 0.0 29.67083 1 GGTATCA 925 0.0 22.124815 1 CAACGCA 3090 0.0 20.859707 5 TCAACGC 3095 0.0 20.826006 4 AACGCAG 3115 0.0 20.762915 6 ATCAACG 3145 0.0 20.564856 3 CGGATCG 205 0.0 20.39044 26 TACGAAT 515 0.0 19.651096 41 CGATTTT 240 0.0 19.250189 31 TAGGACG 470 0.0 19.190443 4 CGAATGC 540 0.0 19.149563 43 CGAGCCG 875 0.0 18.605497 15 TCGATTT 225 0.0 18.577562 30 GTAGGAC 865 0.0 18.565462 3 CGCAATA 535 0.0 18.504852 36 ATACGAA 580 0.0 18.207462 40 TATCAAC 3610 0.0 18.160841 2 CGGACCA 1155 0.0 18.094252 9 TGTAGGA 945 0.0 17.9261 2 TCTAGCG 565 0.0 17.911678 28 >>END_MODULE