FastQCFastQC Report
Thu 2 Feb 2017
SRR4062084_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062084_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741587
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19340.26079205811320855No Hit
TATCAACGCAGAGTACTTTTTTTTT11990.16168028835456932No Hit
GGTATCAACGCAGAGTACTTTTTTT7470.10072992110163745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACTC400.00527390614.2507095
TTATACT801.9942563E-613.0631494
GGTATCA3450.012.937431
TAGGACA1352.7284841E-1112.6672974
TCTAATA604.09747E-412.6630212
GTGTAAC604.099805E-412.6621661
ATTTCTA2000.012.34894715
GTATCAA10050.012.1896961
AAGACGG550.003064694612.0915115
CTAGGGT550.003064694612.0915114
TAATACT658.0113695E-411.6928894
GATACCG658.0113695E-411.6928895
CTAATAC855.3185235E-511.1755173
CGTGAAA1451.207809E-911.1399919
ATTATAC600.00587446311.0823883
TATTAAT600.005883329511.0801432
AGACTGC700.001486670110.8598846
TAGGGTG700.001489186310.8576835
TGAGAAC700.001490865810.8562163
TGTAACA1053.469564E-610.8540172
Produced by FastQC (version 0.11.5)