##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062084_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	741587
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.237377408180024	32.0	32.0	32.0	32.0	32.0
2	30.784529664085266	32.0	32.0	32.0	32.0	32.0
3	30.814028563068124	32.0	32.0	32.0	32.0	32.0
4	30.807701591317	32.0	32.0	32.0	32.0	32.0
5	30.737542594462955	32.0	32.0	32.0	32.0	32.0
6	34.38870422485831	36.0	36.0	36.0	32.0	36.0
7	34.322884570522405	36.0	36.0	36.0	32.0	36.0
8	34.284624730476665	36.0	36.0	36.0	32.0	36.0
9	34.40042907979778	36.0	36.0	36.0	32.0	36.0
10	34.10581900707537	36.0	36.0	36.0	32.0	36.0
11	34.40352514270072	36.0	36.0	36.0	32.0	36.0
12	34.23132147677885	36.0	36.0	36.0	32.0	36.0
13	34.301489912849064	36.0	36.0	36.0	32.0	36.0
14	34.23042340278349	36.0	36.0	36.0	32.0	36.0
15	34.17797507237856	36.0	36.0	36.0	32.0	36.0
16	34.175305122662614	36.0	36.0	36.0	32.0	36.0
17	34.11572883559178	36.0	36.0	36.0	32.0	36.0
18	34.111413765343784	36.0	36.0	36.0	32.0	36.0
19	34.119987270542765	36.0	36.0	36.0	32.0	36.0
20	34.084780342697485	36.0	36.0	36.0	32.0	36.0
21	34.07626886663331	36.0	36.0	36.0	32.0	36.0
22	34.05609186784558	36.0	36.0	36.0	32.0	36.0
23	34.0204278122459	36.0	36.0	36.0	32.0	36.0
24	34.00648339304761	36.0	36.0	36.0	32.0	36.0
25	33.64743853384701	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	12.0
5	59.0
6	167.0
7	44.0
8	127.0
9	120.0
10	59.0
11	15.0
12	36.0
13	39.0
14	171.0
15	200.0
16	290.0
17	458.0
18	533.0
19	720.0
20	1096.0
21	1514.0
22	2191.0
23	3216.0
24	4434.0
25	6219.0
26	8713.0
27	11040.0
28	14519.0
29	19189.0
30	25209.0
31	34468.0
32	48300.0
33	69399.0
34	152632.0
35	336398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24422113805351	17.228034121648044	11.732755110021548	25.794989630276895
2	16.41926897217585	19.525314663437552	38.172646193428676	25.88277017095792
3	19.319456730458047	23.412991650305294	28.030540033226174	29.237011586010485
4	13.04450438300742	15.643020903573838	35.394875252865816	35.917599460552935
5	14.813246047944014	36.335443524617475	33.455996913626045	15.395313513812466
6	34.04285750910462	34.85016333673815	16.977845304389245	14.129133849767983
7	30.301598444756454	30.17172480829463	20.348039745112864	19.17863700183605
8	27.580606620252805	33.748736039053526	19.24946707761429	19.42119026307938
9	27.08774109053595	15.008944906129118	18.72232986113455	39.18098414220038
10	16.438748323865056	26.954680551973347	31.39898695956886	25.20758416459274
11	35.95716378908703	21.64498072235464	22.904678627134555	19.493176861423777
12	24.77587391954282	23.467779723797268	28.995750415573436	22.76059594108647
13	29.410970427671955	20.460686127700715	25.052289320286096	25.076054124341233
14	23.145217907868446	19.924798833504347	25.58378787060539	31.34619538802182
15	24.58098823580248	27.934702762263825	22.407069321263055	25.077239680670633
16	24.86717837589701	26.25594912611135	24.431887991034962	24.44498450695668
17	23.45398303334058	26.529476971601166	25.640367849490097	24.37617214556816
18	23.780805568831394	25.708457203649548	26.774754487008128	23.735982740510927
19	25.186618035738874	25.210915501513192	25.604939404820076	23.997527057927858
20	25.47283908623138	25.117973554486504	25.65843821371301	23.75074914556911
21	25.387522608859975	24.74273142024134	25.24714520962125	24.622600761277436
22	25.40943551086968	25.046842770284133	25.367857489983475	24.175864228862707
23	24.154978621404506	25.371021205839163	25.73731860585644	24.736681566899886
24	24.46978950849322	25.140608866423264	25.876080633296667	24.513520991786848
25	24.52512103963837	25.111591325368853	25.906599376681292	24.45668825831148
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	364.0
1	364.0
2	369.5
3	375.0
4	375.0
5	375.0
6	778.5
7	1182.0
8	1182.0
9	1182.0
10	1288.0
11	1394.0
12	1394.0
13	1394.0
14	1832.5
15	2271.0
16	2271.0
17	2271.0
18	3781.0
19	5291.0
20	5291.0
21	5291.0
22	8221.0
23	11151.0
24	11151.0
25	11151.0
26	16261.5
27	21372.0
28	21372.0
29	21372.0
30	27836.5
31	34301.0
32	34301.0
33	34301.0
34	40668.5
35	47036.0
36	47036.0
37	47036.0
38	55002.5
39	62969.0
40	62969.0
41	62969.0
42	71465.5
43	79962.0
44	79962.0
45	79962.0
46	88029.0
47	96096.0
48	96096.0
49	96096.0
50	99357.0
51	102618.0
52	102618.0
53	102618.0
54	96170.5
55	89723.0
56	89723.0
57	89723.0
58	81022.5
59	72322.0
60	72322.0
61	72322.0
62	61901.0
63	51480.0
64	51480.0
65	51480.0
66	41562.0
67	31644.0
68	31644.0
69	31644.0
70	23776.5
71	15909.0
72	15909.0
73	15909.0
74	11759.0
75	7609.0
76	7609.0
77	7609.0
78	5689.5
79	3770.0
80	3770.0
81	3770.0
82	2654.5
83	1539.0
84	1539.0
85	1539.0
86	1063.5
87	588.0
88	588.0
89	588.0
90	399.5
91	211.0
92	211.0
93	211.0
94	143.5
95	76.0
96	76.0
97	76.0
98	205.0
99	334.0
100	334.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.742297262492465E-4
2	1.3484594524984931E-4
3	4.045378357495479E-4
4	0.01173159723673689
5	0.03559932954596022
6	0.06472605371992766
7	0.11691143453161935
8	0.11596751291487041
9	0.12702488042535806
10	0.14077916684084268
11	0.14725177221283545
12	0.14077916684084268
13	0.13430656146884992
14	0.12230527234161333
15	0.13956555333359402
16	0.12567642097285955
17	0.12918241554935564
18	0.12041742910811543
19	0.10369653189713413
20	0.10019053732063803
21	0.09803300219664046
22	0.10936006159762779
23	0.08886347791965069
24	0.09466185356539422
25	0.0962800049083924
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	741587.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.03815947015158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.69212291139917	51.830289984265
2	12.558828498970925	16.336061813487113
3	3.5106162494940154	6.849720584192917
4	1.4362930074968858	3.7365541466978436
5	0.7460236798152103	2.4260003528165477
6	0.4765417078181301	1.859603735235236
7	0.312908660947042	1.424570237318572
8	0.22722749307572446	1.1822766344529387
9	0.16693675301721678	0.9771533247746749
>10	0.8162533986043559	9.07260641371696
>50	0.038540414466740155	1.6955344893933644
>100	0.016040662555771183	1.6945444662054763
>500	0.0012499217541866172	0.490600903607095
>1k	4.166405847288724E-4	0.4244829138362409
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1934	0.26079205811320855	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1199	0.16168028835456932	No Hit
GGTATCAACGCAGAGTACTTTTTTT	747	0.10072992110163745	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.3484594524984931E-4
13	0.0	0.0	0.0	0.0	1.3484594524984931E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACTC	40	0.005273906	14.250709	5
TTATACT	80	1.9942563E-6	13.063149	4
GGTATCA	345	0.0	12.93743	1
TAGGACA	135	2.7284841E-11	12.667297	4
TCTAATA	60	4.09747E-4	12.663021	2
GTGTAAC	60	4.099805E-4	12.662166	1
ATTTCTA	200	0.0	12.348947	15
GTATCAA	1005	0.0	12.189696	1
AAGACGG	55	0.0030646946	12.091511	5
CTAGGGT	55	0.0030646946	12.091511	4
TAATACT	65	8.0113695E-4	11.692889	4
GATACCG	65	8.0113695E-4	11.692889	5
CTAATAC	85	5.3185235E-5	11.175517	3
CGTGAAA	145	1.207809E-9	11.139991	9
ATTATAC	60	0.005874463	11.082388	3
TATTAAT	60	0.0058833295	11.080143	2
AGACTGC	70	0.0014866701	10.859884	6
TAGGGTG	70	0.0014891863	10.857683	5
TGAGAAC	70	0.0014908658	10.856216	3
TGTAACA	105	3.469564E-6	10.854017	2
>>END_MODULE