FastQCFastQC Report
Thu 2 Feb 2017
SRR4062084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741587
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8230.11097821294062599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA407.0277246E-427.50122336
GATTTCG1950.024.82496541
CTAGCGG450.001397891624.44388429
GTATCAA11300.024.3439311
GTCCTAC3800.022.5860421
GTAGGAC4400.021.9980133
TAAACTA755.4727883E-520.5314774
ATTTCGT2050.020.393942
CTGTAGG4400.020.0062851
TGTAGGA5000.019.7995452
AATCGAG808.979536E-519.25085622
TAGGACC3000.019.0649434
AGTGCGG708.124218E-418.85543610
TCCTACA5200.018.6149582
TAGGACA2500.018.4783294
TAAGGCG600.007413332418.3316765
GATAGTA600.007413332418.3316767
TAGGACG4350.017.6995494
GGTATCA5150.017.5200671
AACGCAG16200.017.3811446
Produced by FastQC (version 0.11.5)