##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062084_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	741587
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.294734130992047	32.0	32.0	32.0	32.0	32.0
2	31.49047245973837	32.0	32.0	32.0	32.0	32.0
3	31.596969741918347	32.0	32.0	32.0	32.0	32.0
4	31.669004445870815	32.0	32.0	32.0	32.0	32.0
5	31.652960475304987	32.0	32.0	32.0	32.0	32.0
6	35.29698740673717	36.0	36.0	36.0	36.0	36.0
7	35.299896033776214	36.0	36.0	36.0	36.0	36.0
8	35.26133279035366	36.0	36.0	36.0	36.0	36.0
9	35.33139469812713	36.0	36.0	36.0	36.0	36.0
10	35.22535319524209	36.0	36.0	36.0	36.0	36.0
11	35.32746259036364	36.0	36.0	36.0	36.0	36.0
12	35.262294241943295	36.0	36.0	36.0	36.0	36.0
13	35.29055795206766	36.0	36.0	36.0	36.0	36.0
14	35.25716470218599	36.0	36.0	36.0	36.0	36.0
15	35.237674069259576	36.0	36.0	36.0	36.0	36.0
16	35.24853051631164	36.0	36.0	36.0	36.0	36.0
17	35.23832537517513	36.0	36.0	36.0	36.0	36.0
18	35.22688099980178	36.0	36.0	36.0	36.0	36.0
19	35.21973281624408	36.0	36.0	36.0	36.0	36.0
20	35.20937529918944	36.0	36.0	36.0	36.0	36.0
21	35.20417968491896	36.0	36.0	36.0	36.0	36.0
22	35.17947455928974	36.0	36.0	36.0	36.0	36.0
23	35.1628291758081	36.0	36.0	36.0	36.0	36.0
24	35.144024908743006	36.0	36.0	36.0	36.0	36.0
25	35.125942067485	36.0	36.0	36.0	36.0	36.0
26	35.097219881146785	36.0	36.0	36.0	36.0	36.0
27	35.07174073979183	36.0	36.0	36.0	36.0	36.0
28	35.046680969326594	36.0	36.0	36.0	36.0	36.0
29	35.033854423014425	36.0	36.0	36.0	36.0	36.0
30	35.0030205491736	36.0	36.0	36.0	36.0	36.0
31	35.003073139092244	36.0	36.0	36.0	36.0	36.0
32	34.977728843682534	36.0	36.0	36.0	36.0	36.0
33	34.967097589359035	36.0	36.0	36.0	36.0	36.0
34	34.940319881551325	36.0	36.0	36.0	36.0	36.0
35	34.93188796459485	36.0	36.0	36.0	36.0	36.0
36	34.9103260979494	36.0	36.0	36.0	36.0	36.0
37	34.882503334065994	36.0	36.0	36.0	36.0	36.0
38	34.87364800084144	36.0	36.0	36.0	32.0	36.0
39	34.824689483499576	36.0	36.0	36.0	32.0	36.0
40	34.832688544971795	36.0	36.0	36.0	32.0	36.0
41	34.80437089646933	36.0	36.0	36.0	32.0	36.0
42	34.78320277998401	36.0	36.0	36.0	32.0	36.0
43	34.76741636517361	36.0	36.0	36.0	32.0	36.0
44	34.723240833509756	36.0	36.0	36.0	32.0	36.0
45	34.69122705764799	36.0	36.0	36.0	32.0	36.0
46	34.63691111090135	36.0	36.0	36.0	32.0	36.0
47	34.61341690186047	36.0	36.0	36.0	32.0	36.0
48	34.60119716230193	36.0	36.0	36.0	32.0	36.0
49	34.56044941456633	36.0	36.0	36.0	32.0	36.0
50	34.18573950190605	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	9.0
21	24.0
22	53.0
23	147.0
24	348.0
25	872.0
26	1862.0
27	3350.0
28	5874.0
29	9391.0
30	13796.0
31	20139.0
32	30120.0
33	50203.0
34	113792.0
35	491605.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.914747243112	17.722726199709978	12.078777863959802	26.28374869321822
2	15.852520517723592	19.990317630273722	37.959069737335376	26.198092114667304
3	18.890349723902183	23.723376281882725	28.410633979921386	28.975640014293706
4	12.692104904751567	16.021451292970347	35.47904696279735	35.80739683948074
5	14.384151825746674	36.690502934921994	33.437883889550385	15.487461349780943
6	34.09323518346465	35.058462459563074	16.89821962898487	13.950082727987411
7	30.421245248366002	30.08574853658438	20.4019218244117	19.091084390637917
8	27.58975009000967	33.75288401765403	19.196938457659048	19.460427434677253
9	27.295785929364996	14.672047918855105	18.710279441252343	39.32188671052756
10	16.326203129235004	26.955839301390128	31.419240089160137	25.298717480214727
11	36.14855708096286	21.461541262185015	22.93540744376587	19.45449421308626
12	24.95448943210902	23.369373208232087	29.090220149787076	22.585917209871813
13	29.497820215295036	20.22406002262715	25.21700083739332	25.061118924684493
14	23.252025723212515	19.74009792512544	25.576634973374667	31.43124137828738
15	24.640804113340714	27.93333755850628	22.38253906824149	25.043319259911513
16	24.958635783182732	26.161602949366426	24.468063588296925	24.411697679153917
17	23.520638846150216	26.327996580306827	25.709727921336267	24.441636652206686
18	23.841771341113965	25.55715123687777	26.940809212699822	23.66026820930844
19	25.291705491061737	24.995448949347818	25.864665912428347	23.848179647162098
20	25.53331787093573	24.77183973764267	25.867872014498694	23.826970376922908
21	25.501660627815752	24.35762762831603	25.478871663068524	24.661840080799692
22	25.470780906353536	24.926677517270395	25.44165418217957	24.1608873941965
23	24.137559045668276	25.08242458403397	25.94301140661851	24.83700496367924
24	24.601086603279228	24.877524518694617	26.077669614888272	24.44371926313788
25	24.66986252418806	24.848533883506274	26.151284073976683	24.330319518328984
26	24.143589937215474	25.330373902349567	26.30895488575805	24.217081274676907
27	24.292865002481175	25.42665429674858	25.889177760037974	24.39130294073227
28	24.202942132311	25.248964697918336	25.674547683229253	24.87354548654141
29	24.340294586835423	25.2414773301905	25.670830293656387	24.747397789317688
30	24.42477470370655	25.15794001717956	25.957854793622797	24.459430485491094
31	24.95462403348517	24.70030286603504	25.632499170691847	24.71257392978794
32	24.543946708379405	25.345748267213246	25.686776881792927	24.42352814261442
33	24.16688579191866	25.236486714267414	26.098019781953518	24.498607711860405
34	24.60897836115445	25.099820249385434	26.09849470659387	24.19270668286625
35	24.63669033219388	25.275052758618365	25.982861034136345	24.10539587505141
36	24.35509839721893	25.333811587597392	25.924977008735333	24.386113006448344
37	24.68631038988534	25.00266324333144	25.48083341311826	24.83019295366496
38	24.470628530435405	25.545620405967203	25.57461228419592	24.409138779401474
39	25.024609550933008	25.111316078329843	25.324643802034032	24.539430568703125
40	25.121968321947826	25.101067172249746	25.73201220087758	24.04495230492485
41	24.84583612579071	24.989987607557367	25.928522979957684	24.23565328669424
42	24.732727047159784	25.434756811279946	25.678565841923284	24.153950299636982
43	24.441148112437887	25.202106356152193	25.887144080424505	24.469601450985415
44	24.244827065397974	25.526867974194662	25.605621723088873	24.622683237318487
45	24.327852228625986	25.567775743077597	25.511409453894263	24.59296257440215
46	24.611200185550523	25.044466050052794	25.446851776686707	24.897481987709973
47	24.89373924581834	25.365035357322014	25.510671923966928	24.23055347289272
48	24.554384147969547	25.669603726208873	25.124669951642353	24.651342174179227
49	24.85985719390748	25.289758820872077	25.15221187623472	24.698172108985723
50	24.437483312611754	25.976633611029452	25.148264916165257	24.437618160193534
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	166.0
1	106.5
2	47.0
3	131.5
4	216.0
5	227.0
6	238.0
7	187.5
8	137.0
9	202.5
10	268.0
11	434.5
12	601.0
13	1011.5
14	1422.0
15	1838.0
16	2254.0
17	2172.0
18	2090.0
19	2018.5
20	1947.0
21	2119.5
22	2292.0
23	2702.5
24	3113.0
25	3842.5
26	4572.0
27	5813.5
28	7055.0
29	8634.5
30	10214.0
31	12098.0
32	13982.0
33	16728.0
34	19474.0
35	22353.0
36	25232.0
37	27535.0
38	29838.0
39	31694.0
40	33550.0
41	35688.5
42	37827.0
43	39697.5
44	41568.0
45	44495.0
46	47422.0
47	49698.5
48	51975.0
49	53519.5
50	55064.0
51	56316.5
52	57569.0
53	57073.5
54	56578.0
55	54447.0
56	52316.0
57	49856.5
58	47397.0
59	43798.5
60	40200.0
61	35310.5
62	30421.0
63	26270.5
64	22120.0
65	18497.0
66	14874.0
67	12344.0
68	9814.0
69	8219.0
70	6624.0
71	5295.5
72	3967.0
73	3414.0
74	2861.0
75	2240.0
76	1619.0
77	1352.0
78	1085.0
79	908.5
80	732.0
81	575.5
82	419.0
83	326.0
84	233.0
85	175.0
86	117.0
87	78.5
88	40.0
89	25.5
90	11.0
91	8.0
92	5.0
93	6.0
94	7.0
95	7.0
96	7.0
97	4.5
98	2.0
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035329637655460516
2	0.0044499161932450276
3	2.6969189049969863E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.3484594524984931E-4
13	0.0
14	0.0
15	0.0
16	5.393837809993973E-4
17	0.0
18	2.6969189049969863E-4
19	0.0
20	4.045378357495479E-4
21	0.0
22	0.0
23	0.0
24	0.001348459452498493
25	2.6969189049969863E-4
26	4.045378357495479E-4
27	4.045378357495479E-4
28	0.002157535123997589
29	8.090756714990958E-4
30	0.0010787675619987945
31	6.742297262492465E-4
32	9.439216167489452E-4
33	2.6969189049969863E-4
34	5.393837809993973E-4
35	2.6969189049969863E-4
36	1.3484594524984931E-4
37	0.001348459452498493
38	0.0
39	6.742297262492465E-4
40	1.3484594524984931E-4
41	8.090756714990958E-4
42	0.0031014567407465343
43	0.0029666107954966848
44	0.004719608083744726
45	0.0012136135072486438
46	0.001348459452498493
47	0.0020226891787477395
48	0.0039105324122456295
49	0.0029666107954966848
50	0.0012136135072486438
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	741587.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.49988503454601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.96747987698473	53.97795698198842
2	12.628462475776606	17.048395305559982
3	3.445692179482284	6.977514779384654
4	1.3634756294772459	3.681377929484775
5	0.7304260048855371	2.4651835678008247
6	0.42057058108377554	1.7033079523240235
7	0.31322837654731195	1.4800015584550732
8	0.21111301326502502	1.1400083299748622
9	0.14753396382132383	0.8962673036967208
>10	0.7354992023255412	8.327263541525403
>50	0.027890911015045858	1.291151757286837
>100	0.008427139166052379	0.9001072190265776
>500	2.006461695449702E-4	0.11146377349194887
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	823	0.11097821294062599	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3484594524984931E-4
2	0.0	0.0	0.0	0.0	1.3484594524984931E-4
3	0.0	0.0	0.0	0.0	1.3484594524984931E-4
4	0.0	0.0	0.0	0.0	1.3484594524984931E-4
5	0.0	0.0	0.0	0.0	1.3484594524984931E-4
6	0.0	0.0	0.0	0.0	2.6969189049969863E-4
7	0.0	0.0	0.0	0.0	2.6969189049969863E-4
8	0.0	0.0	0.0	0.0	2.6969189049969863E-4
9	0.0	0.0	0.0	0.0	2.6969189049969863E-4
10	0.0	0.0	0.0	0.0	4.045378357495479E-4
11	0.0	0.0	0.0	1.3484594524984931E-4	4.045378357495479E-4
12	0.0	0.0	0.0	2.6969189049969863E-4	6.742297262492465E-4
13	0.0	0.0	0.0	2.6969189049969863E-4	0.0012136135072486438
14	0.0	0.0	0.0	2.6969189049969863E-4	0.0012136135072486438
15	0.0	0.0	0.0	2.6969189049969863E-4	0.0014833053977483424
16	0.0	0.0	0.0	4.045378357495479E-4	0.0014833053977483424
17	0.0	0.0	0.0	4.045378357495479E-4	0.0014833053977483424
18	0.0	0.0	0.0	4.045378357495479E-4	0.0014833053977483424
19	0.0	0.0	0.0	4.045378357495479E-4	0.0014833053977483424
20	0.0	0.0	0.0	5.393837809993973E-4	0.0018878432334978902
21	0.0	0.0	0.0	5.393837809993973E-4	0.0020226891787477395
22	0.0	0.0	0.0	6.742297262492465E-4	0.0020226891787477395
23	0.0	0.0	0.0	0.0012136135072486438	0.0020226891787477395
24	0.0	0.0	0.0	0.002157535123997589	0.0020226891787477395
25	0.0	0.0	0.0	0.0029666107954966848	0.0020226891787477395
26	0.0	0.0	0.0	0.003505994576496082	0.0020226891787477395
27	0.0	0.0	0.0	0.004584762138494876	0.0020226891787477395
28	0.0	0.0	0.0	0.005258991864744123	0.0020226891787477395
29	0.0	0.0	0.0	0.007551372933991561	0.0020226891787477395
30	0.0	0.0	0.0	0.010248291838988547	0.0020226891787477395
31	0.0	0.0	0.0	0.01564212964898252	0.0020226891787477395
32	0.0	0.0	0.0	0.02305865663772423	0.0020226891787477395
33	0.0	0.0	0.0	0.030340337681216096	0.0020226891787477395
34	0.0	0.0	0.0	0.0388356322319566	0.0020226891787477395
35	0.0	0.0	0.0	0.050297537578193795	0.0020226891787477395
36	0.0	0.0	0.0	0.061624596979181136	0.0020226891787477395
37	0.0	0.0	0.0	0.0806378752594099	0.0020226891787477395
38	0.0	0.0	0.0	0.10558437513063201	0.0020226891787477395
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	40	7.0277246E-4	27.501223	36
GATTTCG	195	0.0	24.824965	41
CTAGCGG	45	0.0013978916	24.443884	29
GTATCAA	1130	0.0	24.343931	1
GTCCTAC	380	0.0	22.586042	1
GTAGGAC	440	0.0	21.998013	3
TAAACTA	75	5.4727883E-5	20.531477	4
ATTTCGT	205	0.0	20.3939	42
CTGTAGG	440	0.0	20.006285	1
TGTAGGA	500	0.0	19.799545	2
AATCGAG	80	8.979536E-5	19.250856	22
TAGGACC	300	0.0	19.064943	4
AGTGCGG	70	8.124218E-4	18.855436	10
TCCTACA	520	0.0	18.614958	2
TAGGACA	250	0.0	18.478329	4
TAAGGCG	60	0.0074133324	18.331676	5
GATAGTA	60	0.0074133324	18.331676	7
TAGGACG	435	0.0	17.699549	4
GGTATCA	515	0.0	17.520067	1
AACGCAG	1620	0.0	17.381144	6
>>END_MODULE