FastQCFastQC Report
Thu 2 Feb 2017
SRR4062083_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062083_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519139
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17850.1175007685274356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.014.0877071
TCTATAC802.0019233E-613.0609813
TATTAGG957.4038326E-812.9976322
GTAGGAC4500.012.6651943
CTACACG701.09070774E-412.2144774
CTATTAG701.0937329E-412.2108541
ATCGACG550.0030710612.0895043
TTTAAAC951.0411659E-611.9986063
GTATCAA14300.011.88826751
CTAACCC658.0221734E-411.6924914
ACTATAC907.46978E-611.6097613
GATATAC2400.011.4759411
TGTAGGA4400.011.4410972
AACCGCG1251.8280844E-811.4013067
GTTATTC1354.7348294E-911.257953
CGCAAGA2300.011.1501432
CGAACCT2150.011.0463216
CTCTTAC951.3642668E-510.9969091
GTCCTAT3050.010.8985761
CCGTGTA700.001490351610.8580289
Produced by FastQC (version 0.11.5)