##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062083_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1519139
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.465978425937323	32.0	32.0	32.0	14.0	32.0
2	25.841234409754474	32.0	14.0	32.0	14.0	32.0
3	25.817777043443687	32.0	14.0	32.0	14.0	32.0
4	25.654921636532272	32.0	14.0	32.0	14.0	32.0
5	24.968267551553872	32.0	14.0	32.0	14.0	32.0
6	28.909897645969195	36.0	14.0	36.0	14.0	36.0
7	28.662932095088074	36.0	14.0	36.0	14.0	36.0
8	28.189798300221376	36.0	14.0	36.0	14.0	36.0
9	28.959409244315367	36.0	14.0	36.0	14.0	36.0
10	26.955127213507126	32.0	14.0	36.0	14.0	36.0
11	29.484241402531303	36.0	21.0	36.0	14.0	36.0
12	27.941780837698197	36.0	14.0	36.0	14.0	36.0
13	28.7219938399317	36.0	14.0	36.0	14.0	36.0
14	28.011399220216187	32.0	14.0	36.0	14.0	36.0
15	27.776651774459086	32.0	14.0	36.0	14.0	36.0
16	27.897413600730413	36.0	14.0	36.0	14.0	36.0
17	27.65024267035472	32.0	14.0	36.0	14.0	36.0
18	27.742994551518986	32.0	14.0	36.0	14.0	36.0
19	27.807695674984316	32.0	14.0	36.0	14.0	36.0
20	27.826797284514452	32.0	14.0	36.0	14.0	36.0
21	27.86600633648402	36.0	14.0	36.0	14.0	36.0
22	27.757939859354543	32.0	14.0	36.0	14.0	36.0
23	27.587809937076198	32.0	14.0	36.0	14.0	36.0
24	27.530489968330745	32.0	14.0	36.0	14.0	36.0
25	26.977101502890783	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	108.0
5	191.0
6	329.0
7	194.0
8	205.0
9	92.0
10	86.0
11	59.0
12	62.0
13	65.0
14	384.0
15	1210.0
16	3576.0
17	8920.0
18	18550.0
19	29034.0
20	43854.0
21	58994.0
22	75141.0
23	90423.0
24	100984.0
25	111429.0
26	117746.0
27	116918.0
28	118366.0
29	116921.0
30	113108.0
31	108030.0
32	98414.0
33	79427.0
34	71063.0
35	35245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.81228519763628	16.944248974247746	11.461238643986126	24.782227184129855
2	18.810009360571588	18.39411561030005	36.86031157143064	25.935563457697718
3	21.480532594664442	21.730083488630655	27.419791209304805	29.369592707400095
4	14.197159435784473	14.53791127548891	34.85365972479154	36.41126956393508
5	16.92312554983696	35.16895469080067	32.45572330887273	15.452196450489652
6	36.92444394091889	33.73734672477117	15.969737528520916	13.368471805789023
7	32.827484407073406	28.9549032464259	19.278623275552466	18.938989070948228
8	30.121225061086992	32.52542984946373	18.260859250824378	19.092485838624903
9	28.39571914095478	14.20046277776057	18.36809878216032	39.03571929912433
10	18.634923731796412	25.66424062021717	29.840503155266536	25.860332492719884
11	38.974049335858375	20.681761104127624	21.109163644944765	19.235025915069233
12	26.40465138965605	22.67677693130091	27.75423198153365	23.16433969750939
13	31.38892568857709	18.610909536699616	24.441166525949054	25.55899824877424
14	25.275107650819884	18.89573390378053	24.31807967017397	31.511078775225617
15	27.315697021029774	26.27274435037093	21.24497819903964	25.166580429559666
16	27.77699423458668	24.944771189277965	22.936037392145643	24.342197183989708
17	26.083990701825034	25.22166208502413	24.36737413554588	24.32697307760496
18	26.740834366857293	24.299784772205168	25.254800449698244	23.70458041123929
19	27.7249486057667	24.060342101101682	24.258539349533496	23.956169943598123
20	27.77518255677659	23.699931543267752	23.991020901375528	24.533864998580132
21	28.635367244518033	23.378305635516806	23.721643038473424	24.264684081491737
22	28.085973947927485	23.4421867504494	24.20241226798526	24.269427033637854
23	26.62037052287061	23.545284994027966	24.651741309920425	25.182603173181
24	26.89973480044145	23.934243687097464	24.62066579914016	24.545355713320923
25	28.061552571103288	22.828556218506886	24.27315468786237	24.83673652252746
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	346.0
1	346.0
2	371.5
3	397.0
4	397.0
5	397.0
6	865.0
7	1333.0
8	1333.0
9	1333.0
10	1668.0
11	2003.0
12	2003.0
13	2003.0
14	2831.5
15	3660.0
16	3660.0
17	3660.0
18	5938.0
19	8216.0
20	8216.0
21	8216.0
22	13251.5
23	18287.0
24	18287.0
25	18287.0
26	27295.0
27	36303.0
28	36303.0
29	36303.0
30	47168.0
31	58033.0
32	58033.0
33	58033.0
34	71340.0
35	84647.0
36	84647.0
37	84647.0
38	98166.0
39	111685.0
40	111685.0
41	111685.0
42	128215.5
43	144746.0
44	144746.0
45	144746.0
46	161379.0
47	178012.0
48	178012.0
49	178012.0
50	187026.5
51	196041.0
52	196041.0
53	196041.0
54	188322.5
55	180604.0
56	180604.0
57	180604.0
58	168731.0
59	156858.0
60	156858.0
61	156858.0
62	142108.0
63	127358.0
64	127358.0
65	127358.0
66	108088.0
67	88818.0
68	88818.0
69	88818.0
70	70721.5
71	52625.0
72	52625.0
73	52625.0
74	41529.5
75	30434.0
76	30434.0
77	30434.0
78	24470.5
79	18507.0
80	18507.0
81	18507.0
82	13909.0
83	9311.0
84	9311.0
85	9311.0
86	7315.5
87	5320.0
88	5320.0
89	5320.0
90	4095.5
91	2871.0
92	2871.0
93	2871.0
94	2153.5
95	1436.0
96	1436.0
97	1436.0
98	1362.0
99	1288.0
100	1288.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.582676107979586E-4
2	6.582676107979585E-5
3	6.582676107979586E-4
4	0.009544880356570399
5	0.0333741678674565
6	0.06286455683120505
7	0.10690265999358847
8	0.10736344732114704
9	0.11717163472203664
10	0.13119273483203314
11	0.13612974191301783
12	0.13171934892067153
13	0.12612407422888885
14	0.11407777695128622
15	0.1319168292039109
16	0.11947557135982949
17	0.12191116151978193
18	0.11039147833081765
19	0.09604124441542215
20	0.09163085142307584
21	0.09149919790091625
22	0.10288722756772092
23	0.08096691612814891
24	0.09307904016683134
25	0.08866864717448503
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1519139.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.349747836251744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.28238488161693	52.75918081452098
2	10.075039686557256	12.76502447167262
3	2.9972012821399363	5.696158363141661
4	1.2062979852481033	3.056746927233835
5	0.6634052665100807	2.1013278173327494
6	0.39474910848101114	1.5004353888514343
7	0.2674909183474415	1.186183755805848
8	0.17315072133229956	0.8775243627253019
9	0.13345824348946578	0.7609091464554069
>10	0.6988273179289564	7.903329189050372
>50	0.054872885007700496	2.412766521346583
>100	0.04700863166894937	6.036891623906818
>500	0.004848298222546521	1.986317899797813
>1k	0.001264773449359962	0.9572037181586421
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1785	0.1175007685274356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9748028323938758E-4
2	0.0	0.0	0.0	0.0	2.633070443191834E-4
3	0.0	0.0	0.0	0.0	2.633070443191834E-4
4	0.0	0.0	0.0	0.0	2.633070443191834E-4
5	0.0	0.0	0.0	0.0	2.633070443191834E-4
6	0.0	0.0	0.0	0.0	2.633070443191834E-4
7	0.0	0.0	0.0	6.582676107979585E-5	2.633070443191834E-4
8	0.0	0.0	0.0	6.582676107979585E-5	2.633070443191834E-4
9	0.0	0.0	0.0	6.582676107979585E-5	2.633070443191834E-4
10	0.0	0.0	0.0	6.582676107979585E-5	3.291338053989793E-4
11	0.0	0.0	0.0	6.582676107979585E-5	3.291338053989793E-4
12	0.0	0.0	0.0	6.582676107979585E-5	8.557478940373461E-4
13	0.0	0.0	0.0	6.582676107979585E-5	9.87401416196938E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	600	0.0	14.087707	1
TCTATAC	80	2.0019233E-6	13.060981	3
TATTAGG	95	7.4038326E-8	12.997632	2
GTAGGAC	450	0.0	12.665194	3
CTACACG	70	1.09070774E-4	12.214477	4
CTATTAG	70	1.0937329E-4	12.210854	1
ATCGACG	55	0.00307106	12.089504	3
TTTAAAC	95	1.0411659E-6	11.998606	3
GTATCAA	1430	0.0	11.8882675	1
CTAACCC	65	8.0221734E-4	11.692491	4
ACTATAC	90	7.46978E-6	11.609761	3
GATATAC	240	0.0	11.475941	1
TGTAGGA	440	0.0	11.441097	2
AACCGCG	125	1.8280844E-8	11.401306	7
GTTATTC	135	4.7348294E-9	11.25795	3
CGCAAGA	230	0.0	11.150143	2
CGAACCT	215	0.0	11.04632	16
CTCTTAC	95	1.3642668E-5	10.996909	1
GTCCTAT	305	0.0	10.898576	1
CCGTGTA	70	0.0014903516	10.858028	9
>>END_MODULE