FastQCFastQC Report
Thu 2 Feb 2017
SRR4062083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519139
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17610.1159209262615205No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16560.10900911634814195No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16390.10789006140978542No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15580.10255809376232194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18800.025.1674231
GGTATCA9150.024.0512221
TAGGACG4050.022.2704894
GATATAC3900.020.8783071
CGCAATA3150.020.25428636
TATACAC5150.018.7951743
ATACGAA3450.018.49487140
GTAGGAC4300.018.4176833
CGAATGC3750.018.1887543
CCTATAC851.4316881E-418.116743
AGGACGT5050.017.860495
TACGAAT3700.017.8398841
CGAGCCG5200.017.76834115
CAATACG3650.017.48087738
CGGACCA6350.017.3219669
GCGAAAG6100.017.31060818
GGTATAG1408.346433E-817.2911071
GGCGTTA5100.017.25744642
TCAACGC26950.017.1419984
ATCAACG27150.017.0967483
Produced by FastQC (version 0.11.5)