##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062083_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1519139
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.261690339067062	32.0	32.0	32.0	32.0	32.0
2	31.412960894296045	32.0	32.0	32.0	32.0	32.0
3	31.558844187398257	32.0	32.0	32.0	32.0	32.0
4	31.64080245454827	32.0	32.0	32.0	32.0	32.0
5	31.607193285143754	32.0	32.0	32.0	32.0	32.0
6	35.24863294273928	36.0	36.0	36.0	36.0	36.0
7	35.25582254158441	36.0	36.0	36.0	36.0	36.0
8	35.19925102311243	36.0	36.0	36.0	36.0	36.0
9	35.2956240344037	36.0	36.0	36.0	36.0	36.0
10	35.16991203569917	36.0	36.0	36.0	36.0	36.0
11	35.29700902945682	36.0	36.0	36.0	36.0	36.0
12	35.211132095219725	36.0	36.0	36.0	36.0	36.0
13	35.25195521937097	36.0	36.0	36.0	36.0	36.0
14	35.2159157259474	36.0	36.0	36.0	36.0	36.0
15	35.18942111288039	36.0	36.0	36.0	36.0	36.0
16	35.20188211875279	36.0	36.0	36.0	36.0	36.0
17	35.18134680236634	36.0	36.0	36.0	36.0	36.0
18	35.1822222982887	36.0	36.0	36.0	36.0	36.0
19	35.17462589006009	36.0	36.0	36.0	36.0	36.0
20	35.1708954875097	36.0	36.0	36.0	36.0	36.0
21	35.16403568073758	36.0	36.0	36.0	36.0	36.0
22	35.141201693854214	36.0	36.0	36.0	36.0	36.0
23	35.116875414297176	36.0	36.0	36.0	36.0	36.0
24	35.10152922148664	36.0	36.0	36.0	36.0	36.0
25	35.0789644660561	36.0	36.0	36.0	36.0	36.0
26	35.034615002313814	36.0	36.0	36.0	36.0	36.0
27	35.02390630482135	36.0	36.0	36.0	36.0	36.0
28	35.00185894773289	36.0	36.0	36.0	36.0	36.0
29	34.97787365079825	36.0	36.0	36.0	36.0	36.0
30	34.95604878816224	36.0	36.0	36.0	36.0	36.0
31	34.959202548285575	36.0	36.0	36.0	36.0	36.0
32	34.9378430808504	36.0	36.0	36.0	36.0	36.0
33	34.91249714476424	36.0	36.0	36.0	32.0	36.0
34	34.90638776306842	36.0	36.0	36.0	32.0	36.0
35	34.890037712151425	36.0	36.0	36.0	32.0	36.0
36	34.86808317079609	36.0	36.0	36.0	32.0	36.0
37	34.85889375494935	36.0	36.0	36.0	32.0	36.0
38	34.83390525817585	36.0	36.0	36.0	32.0	36.0
39	34.81445147547394	36.0	36.0	36.0	32.0	36.0
40	34.807513334856125	36.0	36.0	36.0	32.0	36.0
41	34.78265846640762	36.0	36.0	36.0	32.0	36.0
42	34.751512534402714	36.0	36.0	36.0	32.0	36.0
43	34.7523649909587	36.0	36.0	36.0	32.0	36.0
44	34.69958048605164	36.0	36.0	36.0	32.0	36.0
45	34.682912491878625	36.0	36.0	36.0	32.0	36.0
46	34.659436694074735	36.0	36.0	36.0	32.0	36.0
47	34.63667248355812	36.0	36.0	36.0	32.0	36.0
48	34.61150362145926	36.0	36.0	36.0	32.0	36.0
49	34.59990428788939	36.0	36.0	36.0	32.0	36.0
50	34.18543069462373	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	12.0
21	37.0
22	96.0
23	258.0
24	646.0
25	1557.0
26	3308.0
27	6415.0
28	11272.0
29	18670.0
30	28299.0
31	42873.0
32	65989.0
33	113405.0
34	259014.0
35	967284.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.91384053743318	18.034525946480567	12.167972891284622	25.883660624801628
2	16.068691938235975	19.815584778075035	37.50743875208022	26.608284531608774
3	18.890157885902177	23.192013876317773	28.40221573461213	29.515612503167922
4	12.622092265482134	15.975375509005765	35.32516466575124	36.077367559760866
5	14.641526971216571	36.78849452781775	33.01319564121232	15.55678285975336
6	35.154781754664974	34.93650021492438	16.28791045454037	13.620807575870279
7	30.778881985124467	30.092769654389755	19.906341684335665	19.22200667615011
8	28.07596934842697	33.788020714365175	18.69249621002423	19.44351372718362
9	26.885360720776703	14.462731850080868	18.596652445892047	40.055254983250386
10	16.685833225267736	26.838162933082486	30.618988782461642	25.857015059188132
11	37.457204376952994	21.243809815954958	21.712496354843104	19.58648945224894
12	24.380191674362912	23.58105479485419	28.636220911977112	23.402532618805786
13	29.633101381769546	19.238660846703297	25.152932022678637	25.975305748848527
14	23.40088694977879	19.59557354527795	24.957229061988404	32.046310442954855
15	25.214348390766084	27.402759062863897	21.838422948788754	25.54446959758126
16	25.882872587767224	25.902554880023594	23.55384522214001	24.660727310069174
17	24.147033286618274	26.312733726143563	24.82913018492712	24.711102802311046
18	24.80243717321084	25.21444741323528	25.956052679909714	24.027062733644165
19	25.66486674359621	25.01904039064233	25.05122967681035	24.26486318895111
20	25.88079787563177	24.595394481329492	24.73619838671243	24.787609256326302
21	26.65788098250455	24.440439249100475	24.38698788391838	24.51469188447659
22	25.952332209231677	24.458986307375426	24.959664652148355	24.62901683124454
23	24.530358663926517	24.428129636675536	25.49300985163955	25.5485018477584
24	24.738089705468997	24.890084897444385	25.384052814544145	24.987772582542473
25	24.958166955647098	24.520022591818762	25.296451794245932	25.22535865828821
26	24.525401760353148	25.516365649481575	25.5710022927642	24.38723029740107
27	25.073496014166004	24.822694568601765	25.114045539223106	24.989763878009125
28	24.67084237100135	24.933299541506347	25.422929948884377	24.97292813860793
29	24.630315206315185	24.996576992651068	25.38778723639552	24.98532056463823
30	24.577221782639665	25.105949636565427	25.59662909662054	24.72019948417437
31	25.16453496409129	24.798338522707077	24.820917235522963	25.21620927767867
32	25.18821764456899	24.88442314262411	24.649155201139607	25.278204011667288
33	24.64035336014693	24.63587711387439	25.324165805428105	25.39960372055058
34	25.23497063443059	24.901686891014965	25.36576952800492	24.49757294654953
35	25.507558911853728	24.66207720977873	25.55396693115946	24.27639694720808
36	24.40156773323784	25.35013323362754	25.071553830598447	25.17674520253618
37	25.46336280669233	25.017773541384702	24.906129370316343	24.612734281606624
38	24.798915701591493	24.72630878412048	25.311837823925266	25.162937690362764
39	25.566195410402393	24.550285656734015	24.79088373550405	25.09263519735954
40	25.692135934984183	24.81117581154576	25.049600497124025	24.447087756346033
41	24.53792387971473	25.194158204541832	25.67160504554605	24.59631287019739
42	25.8041284551636	25.303163059957196	25.021279509067078	23.871428975812126
43	25.184340124563303	24.418936096959516	25.319554700352253	25.07716907812493
44	24.550334907756366	25.23134144133766	24.991326958840084	25.226996692065896
45	24.808028411465962	25.282979122575465	24.979905931453057	24.929086534505515
46	24.66807758515705	25.05922895389061	24.93066641213083	25.342027048821507
47	24.980366935879367	24.970492804720624	25.258422469309554	24.79071779009045
48	25.68917075688315	25.314204988308653	24.322876071706936	24.673748183101264
49	24.9508415362851	25.441274150514758	24.6168865494412	24.990997763758937
50	25.023763945683914	25.627144350500426	24.524589430112933	24.824502273702727
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	143.0
1	92.0
2	41.0
3	100.5
4	160.0
5	164.5
6	169.0
7	152.5
8	136.0
9	175.5
10	215.0
11	370.0
12	525.0
13	1009.0
14	1493.0
15	1882.5
16	2272.0
17	2418.5
18	2565.0
19	2565.0
20	2565.0
21	3075.0
22	3585.0
23	4638.5
24	5692.0
25	7389.5
26	9087.0
27	12058.0
28	15029.0
29	17560.0
30	20091.0
31	22513.5
32	24936.0
33	28664.5
34	32393.0
35	37767.0
36	43141.0
37	49901.5
38	56662.0
39	61921.0
40	67180.0
41	71699.0
42	76218.0
43	79636.0
44	83054.0
45	90780.5
46	98507.0
47	104879.0
48	111251.0
49	115286.0
50	119321.0
51	116064.5
52	112808.0
53	112215.5
54	111623.0
55	112443.5
56	113264.0
57	108838.0
58	104412.0
59	96167.0
60	87922.0
61	76516.0
62	65110.0
63	56086.5
64	47063.0
65	39601.0
66	32139.0
67	27573.5
68	23008.0
69	20539.5
70	18071.0
71	13865.0
72	9659.0
73	8535.5
74	7412.0
75	5302.5
76	3193.0
77	2872.0
78	2551.0
79	2200.0
80	1849.0
81	1489.0
82	1129.0
83	960.0
84	791.0
85	615.5
86	440.0
87	294.0
88	148.0
89	96.5
90	45.0
91	32.0
92	19.0
93	15.5
94	12.0
95	16.5
96	21.0
97	16.0
98	11.0
99	9.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03376912843393527
2	0.004410392992346322
3	2.633070443191834E-4
4	6.582676107979585E-5
5	6.582676107979585E-5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.6078732755857097E-4
17	0.0
18	1.316535221595917E-4
19	0.0
20	3.291338053989793E-4
21	6.582676107979585E-5
22	0.0
23	6.582676107979585E-5
24	7.899211329575502E-4
25	3.291338053989793E-4
26	7.899211329575502E-4
27	5.924408497181627E-4
28	0.002238109876713059
29	4.6078732755857097E-4
30	0.0013823619826757129
31	5.924408497181627E-4
32	0.0013165352215959172
33	5.924408497181627E-4
34	8.557478940373461E-4
35	3.291338053989793E-4
36	1.9748028323938755E-4
37	0.0017773225491544882
38	0.0
39	5.266140886383668E-4
40	6.582676107979585E-5
41	0.0011190549383565296
42	0.004739526797745301
43	0.004739526797745301
44	0.004212912709106935
45	0.002238109876713059
46	0.002238109876713059
47	0.0011848816994363253
48	0.004410392992346322
49	0.004739526797745301
50	0.0020406295934736716
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1519139.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.72704072377188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00299396650298	40.29688911243123
2	14.738796240851551	15.837438117032146
3	4.614657546665461	7.437956818078694
4	1.9542666822732004	4.1998786249441125
5	1.0131851582732827	2.7217720129634957
6	0.6100423150723023	1.96654609830682
7	0.4063062535384846	1.5280742841129744
8	0.2793726192550187	1.2007891273456965
9	0.1948689703282111	0.9422759794160964
>10	1.0353179072820502	9.82406166883973
>50	0.07888911576127602	2.895594466441921
>100	0.06206717364924376	6.957667896487033
>500	0.007019398415743452	2.626423193716012
>1k	0.002216652131287406	1.564632599884025
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1761	0.1159209262615205	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1656	0.10900911634814195	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1639	0.10789006140978542	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1558	0.10255809376232194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9748028323938758E-4
2	0.0	0.0	0.0	0.0	1.9748028323938758E-4
3	0.0	0.0	0.0	0.0	1.9748028323938758E-4
4	0.0	0.0	0.0	0.0	1.9748028323938758E-4
5	0.0	0.0	0.0	0.0	1.9748028323938758E-4
6	0.0	0.0	0.0	0.0	4.60787327558571E-4
7	0.0	0.0	0.0	0.0	4.60787327558571E-4
8	0.0	0.0	0.0	6.582676107979585E-5	5.266140886383668E-4
9	0.0	0.0	0.0	6.582676107979585E-5	5.266140886383668E-4
10	0.0	0.0	0.0	6.582676107979585E-5	5.266140886383668E-4
11	0.0	0.0	0.0	6.582676107979585E-5	5.266140886383668E-4
12	0.0	0.0	0.0	6.582676107979585E-5	0.0011848816994363255
13	0.0	0.0	0.0	6.582676107979585E-5	0.001382361982675713
14	0.0	0.0	0.0	6.582676107979585E-5	0.001382361982675713
15	0.0	0.0	0.0	6.582676107979585E-5	0.0014481887437555088
16	0.0	0.0	0.0	6.582676107979585E-5	0.0015140155048353047
17	0.0	0.0	0.0	1.316535221595917E-4	0.0015140155048353047
18	0.0	0.0	0.0	2.633070443191834E-4	0.0016456690269948965
19	0.0	0.0	0.0	2.633070443191834E-4	0.0016456690269948965
20	0.0	0.0	0.0	2.633070443191834E-4	0.0019089760713140798
21	0.0	0.0	0.0	2.633070443191834E-4	0.0020406295934736716
22	0.0	0.0	0.0	3.9496056647877516E-4	0.0021722831156332635
23	0.0	0.0	0.0	4.60787327558571E-4	0.0021722831156332635
24	0.0	0.0	0.0	4.60787327558571E-4	0.0021722831156332635
25	0.0	0.0	0.0	7.240943718777544E-4	0.0021722831156332635
26	0.0	0.0	0.0	8.557478940373461E-4	0.002238109876713059
27	0.0	0.0	0.0	0.0010532281772767337	0.002238109876713059
28	0.0	0.0	0.0	0.0011190549383565296	0.002238109876713059
29	0.0	0.0	0.0	0.0011848816994363255	0.002238109876713059
30	0.0	0.0	0.0	0.0021722831156332635	0.002238109876713059
31	0.0	0.0	0.0	0.0034229915761493845	0.0025014169210322425
32	0.0	0.0	0.0	0.005002833842064485	0.0025014169210322425
33	0.0	0.0	0.0	0.006385195824740199	0.0025672436821120386
34	0.0	0.0	0.0	0.008228345134974483	0.0029622042485908137
35	0.0	0.0	0.0	0.009808187400889583	0.0030280310096706094
36	0.0	0.0	0.0	0.013297005738118763	0.0030280310096706094
37	0.0	0.0	0.0	0.017970705774784268	0.0030280310096706094
38	0.0	0.0	0.0	0.023302673422247733	0.0031596845318302012
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1880	0.0	25.167423	1
GGTATCA	915	0.0	24.051222	1
TAGGACG	405	0.0	22.270489	4
GATATAC	390	0.0	20.878307	1
CGCAATA	315	0.0	20.254286	36
TATACAC	515	0.0	18.795174	3
ATACGAA	345	0.0	18.494871	40
GTAGGAC	430	0.0	18.417683	3
CGAATGC	375	0.0	18.18875	43
CCTATAC	85	1.4316881E-4	18.11674	3
AGGACGT	505	0.0	17.86049	5
TACGAAT	370	0.0	17.83988	41
CGAGCCG	520	0.0	17.768341	15
CAATACG	365	0.0	17.480877	38
CGGACCA	635	0.0	17.321966	9
GCGAAAG	610	0.0	17.310608	18
GGTATAG	140	8.346433E-8	17.291107	1
GGCGTTA	510	0.0	17.257446	42
TCAACGC	2695	0.0	17.141998	4
ATCAACG	2715	0.0	17.096748	3
>>END_MODULE