FastQCFastQC Report
Thu 2 Feb 2017
SRR4062082_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062082_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960162
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT73400.7644543316648649No Hit
CTGTAGGACGTGGAATATGGCAAGA71960.7494568624877885No Hit
GTCCTACAGTGGACATTTCTAAATT71830.7481029242981914No Hit
CTTTAGGACGTGAAATATGGCGAGG67430.7022773240349024No Hit
GTCCTACAGTGTGCATTTCTCATTT42720.44492491891993224No Hit
CTGTAGGACCTGGAATATGGCGAGA34830.36275128572053467No Hit
CTGAAGGACCTGGAATATGGCGAGA28640.29828299807740777No Hit
ATTTAGAAATGTCCACTGTAGGACG28110.2927630962275116No Hit
GTCCTTCAGTGTGCATTTCTCATTT26710.27818222341646515No Hit
GATATACACTGTTCTACAAATCCCG25750.26818391063174757No Hit
TTGTAGAACAGTGTATATCAATGAG25730.2679756124487326No Hit
GAATATGGCAAGAAAACTGAAAATC22560.2349603504408631No Hit
GCGCAAGACGGACCAGAGCGAAAGC21380.22267075764298105No Hit
TTTCTAAATTTTCCACCTTTTTCAG21280.22162926672790634No Hit
GATTAAGAGGGACGGCCGGGGGCAT20830.21694255761006997No Hit
GAATAGGACCGCGGTTCTATTTTGT20720.21579691760348774No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19640.20454881572068045No Hit
TCGTAGTTCCGACCATAAACGATGC19430.2023616847990235No Hit
ATCAGATACCGTCGTAGTTCCGACC19050.19840401932173946No Hit
GTGTATATCAATGAGTTACAATGAG19020.19809157204721703No Hit
CTGTAGGACATGGAATATGGCAAGA18460.19225922292279843No Hit
GTATCTGATCGTCTTCGAACCTCCG18280.1903845392756639No Hit
GTGTATATCAATGAGTTACAATGAA18220.18975964472661905No Hit
GGAATATGGCGAGAAAACTGAAAAT17900.18642687379837986No Hit
GGGTAGGCACACGCTGAGCCAGTCA17500.18226091013808088No Hit
GTATCAACGCAGAGTACTTTTTTTT17380.18101112103999117No Hit
GAACTACGACGGTATCTGATCGTCT17040.17747005192873702No Hit
ATTCCAGGTCCTTCAGTGTGCATTT17010.1771576046542146No Hit
GAATAACGCCGCCGCATCGCCAGTC16900.17601196464763239No Hit
GTCTTGCGCCGGTCCAAGAATTTCA16100.16768003732703438No Hit
GAAATATGGCGAGGAAAACTGAAAA15970.16632609913743723No Hit
ACCATACTCCCCCCGGAACCCAAAG15500.1614310918365859No Hit
TTCCAGGTCCTTCAGTGTGCATTTC15480.16122279365357095No Hit
GTTCTACAGTGTGGTTTTTATCATT15360.15997300455548127No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT15300.15934811000643642No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC14880.15497384816312246No Hit
GTACATGGGGAATAATTGCAATCCC14270.1486207535811665No Hit
GTGCATGGCCGTTCTTAGTTGGTGG14210.14799585903212165No Hit
GTCCTATTCCATTATTCCTAGCTGC14110.14695436811704693No Hit
GCTTTGAACACTCTAATTTTTTCAA13890.14466308810388248No Hit
ACCTGGAATATGGCGAGAAAACTGA13440.1399763789860461No Hit
CTATTGGAGCTGGAATTACCGCGGC13410.13966393171152366No Hit
GTTCTACAAATCCCGTTTCCAACGA13340.13893488807097135No Hit
GAATAATGGAATAGGACCGCGGTTC13330.13883073897946388No Hit
GACCTGGAATATGGCGAGAAAACTG13220.13768509897288167No Hit
GAGTATGGTTGCAAAGCTGAAACTT12950.13487307350217984No Hit
CCATATTTCACGTCCTAAAGTGTGT12810.1334149862210752No Hit
GCCATATTCCACGTCCTACAGTGGA12700.132269346214493No Hit
TATCAACGCAGAGTACTTTTTTTTT12600.13122785529941822No Hit
CCATATTCCAGGTCCTTCAGTGTGC12370.1288324261947463No Hit
GTCCACTGTAGGACGTGGAATATGG12200.12706189163911924No Hit
ACAGTGGACATTTCTAAATTTTCCA12160.12664529527308932No Hit
GTTATTGCTCAATCTCGGGTGGCTG12120.12622869890705943No Hit
GCCATGCACCACCACCCACGGAATC11800.12289592797882024No Hit
GATATACACTGTTCTACAATGCCGG11790.12279177888731277No Hit
GTATCAACGCAGAGTACATGGGGAA11790.12279177888731277No Hit
CATCTAAGGGCATCACAGACCTGTT11770.12258348070429781No Hit
ATATTAAAGTTGCTGCAGTTAAAAA11760.12247933161279034No Hit
ATACACACTTTAGGACGTGAAATAT11720.12206273524676044No Hit
GGATTGACAGATTGATAGCTCTTTC11370.11841751704399883No Hit
CCTAAAGTGTGTATTTCTCATTTTC11190.11654283339686428No Hit
CACTTTAGGACGTGAAATATGGCGA10820.11268931701108771No Hit
CTCTAGATAACCTCGGGCCGATCGC10710.11154367700450549No Hit
GTCCTGTATTGTTATTTTTCGTCAC10610.11050218608943073No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG10600.11039803699792326No Hit
TCACAGACCTGTTATTGCTCAATCT10560.10998144063189337No Hit
GTTCTATTTTGTTGGTTTTCGGAAC10430.1086275024422962No Hit
CCACTGTAGGACGTGGAATATGGCA10340.10769016061872892No Hit
GTAGGACGTGGAATATGGCAAGAAA10330.10758601152722144No Hit
CTTTAATATACGCTATTGGAGCTGG10310.10737771334420651No Hit
CTCCAATAGCGTATATTAAAGTTGC10200.10623207333762429No Hit
ATACAGGACTCTTTCGAGGCCCTGT10170.10591962606310186No Hit
CTCGGGGGTCGCGTAACTAGTTAGC10110.105294731514057No Hit
GTGTATTTCTCATTTTCCGTGATTT10090.10508643333104206No Hit
ATCTCGGGTGGCTGAACGCCACTTG9790.10196196058581782No Hit
CTCTTAATCATGGCCTCAGTTCCGA9780.10185781149431035No Hit
TCTAAGGGCATCACAGACCTGTTAT9750.1015453642197879No Hit
GTCCTACAGTGGACATTTCTAAATA9740.10144121512828043No Hit
GAAATACACACTTTAGGACGTGAAA9730.10133706603677296No Hit
TCCTAAAGTGTGTATTTCTCATTTT9670.10071217148772812No Hit
GCTAAGAGCATCGAGGGGGCGCCGA9650.10050387330471316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTC651.0000804E-817.5401488
TAAGACC607.6812285E-817.4156154
CGAACGA508.716754E-515.19828916
GGTATCA7150.015.0060341
GACCTAG704.4260014E-714.9300067
CGTCTTA456.7567313E-414.77842715
GTAAGAC851.7174898E-814.5270193
GTGTAGG851.7258571E-814.5217151
GGACTGT602.563908E-514.2513696
GACCGGG400.00527378414.2513697
ACCTGCA400.00527378414.2513698
TTGTAGA3350.014.1715931
CCAACGA2100.014.02076719
CACCTTT6650.014.00134614
CGAGACT759.668147E-713.9324924
AGCCCCG2250.013.93176616
TGGTCAT551.9538583E-413.82239510
CCACCTT6700.013.75577113
CGTCACT903.6308847E-813.72282515
AAACGGG903.643254E-813.71924619
Produced by FastQC (version 0.11.5)