##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062082_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	960162
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.460018205261196	32.0	32.0	32.0	32.0	32.0
2	31.169481816610112	32.0	32.0	32.0	32.0	32.0
3	31.174785088349676	32.0	32.0	32.0	32.0	32.0
4	31.181264203332354	32.0	32.0	32.0	32.0	32.0
5	31.182420258248086	32.0	32.0	32.0	32.0	32.0
6	34.8861629600005	36.0	36.0	36.0	36.0	36.0
7	34.799735877903935	36.0	36.0	36.0	36.0	36.0
8	34.80639517081492	36.0	36.0	36.0	36.0	36.0
9	34.82499828153999	36.0	36.0	36.0	36.0	36.0
10	34.63150489188283	36.0	36.0	36.0	32.0	36.0
11	34.786744320229296	36.0	36.0	36.0	36.0	36.0
12	34.6476761213212	36.0	36.0	36.0	32.0	36.0
13	34.72255202767866	36.0	36.0	36.0	32.0	36.0
14	34.68854526631964	36.0	36.0	36.0	32.0	36.0
15	34.656309039516245	36.0	36.0	36.0	32.0	36.0
16	34.64355077580658	36.0	36.0	36.0	32.0	36.0
17	34.583995200809866	36.0	36.0	36.0	32.0	36.0
18	34.595035004509654	36.0	36.0	36.0	32.0	36.0
19	34.501105021860894	36.0	36.0	36.0	32.0	36.0
20	34.482092605206205	36.0	36.0	36.0	32.0	36.0
21	34.41792843291028	36.0	36.0	36.0	32.0	36.0
22	34.346204078061824	36.0	36.0	36.0	32.0	36.0
23	34.37441077651479	36.0	36.0	36.0	32.0	36.0
24	34.30811154784297	36.0	36.0	36.0	32.0	36.0
25	33.98073345956203	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	10.0
5	80.0
6	198.0
7	34.0
8	167.0
9	146.0
10	68.0
11	19.0
12	43.0
13	42.0
14	103.0
15	173.0
16	246.0
17	312.0
18	465.0
19	579.0
20	897.0
21	1188.0
22	1828.0
23	2508.0
24	3650.0
25	5249.0
26	7432.0
27	9705.0
28	13214.0
29	18218.0
30	24606.0
31	35004.0
32	51658.0
33	78090.0
34	193433.0
35	510796.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.96110820068927	20.031745006774976	12.842250854292711	25.16489593824305
2	12.770411505618352	20.413953134747757	45.98070940481609	20.834925954817805
3	19.0812742929274	25.624377705289557	29.659347022775467	25.63500097900758
4	10.72902811181396	16.939375600874527	39.11030467919159	33.22129160811992
5	11.820555349500118	40.044362114878915	35.027213209033285	13.107869326587682
6	31.20876257770691	38.681653092383286	18.10887565331124	12.000708676598562
7	28.018320846270463	31.063384789902475	21.910885535372827	19.00740882845424
8	23.768478299402148	41.40776020802879	19.718110140986635	15.105651351582425
9	26.975460359403076	13.867248396471666	20.951970793938134	38.20532045018712
10	17.592997218453117	27.352607739312635	31.608572629453523	23.44582241278072
11	36.0021694462697	19.540035670703087	27.40584290288599	17.051951980141222
12	26.87844814100224	22.9959847734265	32.00750899515044	18.118058090420817
13	30.687928351833545	21.679243472783625	25.810609568384034	21.822218606998796
14	20.072240336760093	25.92977630312579	25.176195307506298	28.821788052607815
15	21.050847669742527	38.629252949323366	20.768117272105325	19.55178210882878
16	20.08976241324079	26.52923535104111	33.37351508275494	20.00748715296316
17	19.28566214426496	31.25473183652613	29.378682489858488	20.08092352935042
18	20.487403579673064	24.988270033219266	36.58373319486759	17.94059319224008
19	25.884510369546383	24.117626821434044	25.874919073439095	24.122943735580478
20	25.505677831905317	27.139546544877962	27.46313031856948	19.89164530464724
21	24.15030414227708	25.561086468144552	24.481602910592073	25.807006478986295
22	24.73907264055047	30.886126097948758	26.077827299502175	18.296973961998592
23	19.473679273111614	30.305917277566586	29.40887680234152	20.81152664698028
24	24.834899980922934	26.344490115890572	30.079425227804148	18.74118467538235
25	21.628321277755713	28.451997118753436	29.833932203142478	20.085749400348377
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	592.0
1	592.0
2	471.5
3	351.0
4	351.0
5	351.0
6	721.0
7	1091.0
8	1091.0
9	1091.0
10	1243.0
11	1395.0
12	1395.0
13	1395.0
14	1985.5
15	2576.0
16	2576.0
17	2576.0
18	4975.5
19	7375.0
20	7375.0
21	7375.0
22	16262.5
23	25150.0
24	25150.0
25	25150.0
26	44054.5
27	62959.0
28	62959.0
29	62959.0
30	85401.5
31	107844.0
32	107844.0
33	107844.0
34	113841.5
35	119839.0
36	119839.0
37	119839.0
38	116218.0
39	112597.0
40	112597.0
41	112597.0
42	112899.0
43	113201.0
44	113201.0
45	113201.0
46	113358.0
47	113515.0
48	113515.0
49	113515.0
50	100557.0
51	87599.0
52	87599.0
53	87599.0
54	67103.5
55	46608.0
56	46608.0
57	46608.0
58	45111.0
59	43614.0
60	43614.0
61	43614.0
62	42248.5
63	40883.0
64	40883.0
65	40883.0
66	35230.5
67	29578.0
68	29578.0
69	29578.0
70	22897.5
71	16217.0
72	16217.0
73	16217.0
74	13650.0
75	11083.0
76	11083.0
77	11083.0
78	10202.5
79	9322.0
80	9322.0
81	9322.0
82	6415.0
83	3508.0
84	3508.0
85	3508.0
86	2792.5
87	2077.0
88	2077.0
89	2077.0
90	1401.0
91	725.0
92	725.0
93	725.0
94	422.0
95	119.0
96	119.0
97	119.0
98	231.5
99	344.0
100	344.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011456400065822227
2	5.207454575373738E-4
3	6.248945490448487E-4
4	0.010727356425269903
5	0.03478579656349658
6	0.06530148037518668
7	0.10789845880174387
8	0.11081463336395317
9	0.12320837525334266
10	0.13987222989453862
11	0.1449755353784049
12	0.13664360805780693
13	0.13060296075037336
14	0.11883411341002872
15	0.13518552077670226
16	0.1235208225278651
17	0.12831168073720892
18	0.11248101882807275
19	0.09987897875566833
20	0.09425492781426467
21	0.09446322599727963
22	0.10279515331787761
23	0.0817570368333677
24	0.09321343689918993
25	0.08904747323889094
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	960162.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.218038730507143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41801419617386	19.982943539083827
2	13.991320733341118	7.616326192220522
3	4.599547233617309	3.755719642421787
4	2.1023917735576707	2.288919228775691
5	1.182997689385795	1.6099438463901514
6	0.7445830965022939	1.2159654935208224
7	0.5224909872063447	0.995482594828624
8	0.3914413412689624	0.8523412469904226
9	0.28449594569667336	0.696907950177988
>10	2.038092991489276	11.152361870159512
>50	0.30407299424428197	5.86673533286171
>100	0.34328341740602764	20.231034609499023
>500	0.04882082196031972	9.088098362854593
>1k	0.026909114466317954	11.669227532120416
>5k	0.0015376636837895975	2.977992558094929
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	7340	0.7644543316648649	No Hit
CTGTAGGACGTGGAATATGGCAAGA	7196	0.7494568624877885	No Hit
GTCCTACAGTGGACATTTCTAAATT	7183	0.7481029242981914	No Hit
CTTTAGGACGTGAAATATGGCGAGG	6743	0.7022773240349024	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4272	0.44492491891993224	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3483	0.36275128572053467	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2864	0.29828299807740777	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2811	0.2927630962275116	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2671	0.27818222341646515	No Hit
GATATACACTGTTCTACAAATCCCG	2575	0.26818391063174757	No Hit
TTGTAGAACAGTGTATATCAATGAG	2573	0.2679756124487326	No Hit
GAATATGGCAAGAAAACTGAAAATC	2256	0.2349603504408631	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2138	0.22267075764298105	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2128	0.22162926672790634	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2083	0.21694255761006997	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2072	0.21579691760348774	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1964	0.20454881572068045	No Hit
TCGTAGTTCCGACCATAAACGATGC	1943	0.2023616847990235	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1905	0.19840401932173946	No Hit
GTGTATATCAATGAGTTACAATGAG	1902	0.19809157204721703	No Hit
CTGTAGGACATGGAATATGGCAAGA	1846	0.19225922292279843	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1828	0.1903845392756639	No Hit
GTGTATATCAATGAGTTACAATGAA	1822	0.18975964472661905	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1790	0.18642687379837986	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1750	0.18226091013808088	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1738	0.18101112103999117	No Hit
GAACTACGACGGTATCTGATCGTCT	1704	0.17747005192873702	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1701	0.1771576046542146	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1690	0.17601196464763239	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1610	0.16768003732703438	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1597	0.16632609913743723	No Hit
ACCATACTCCCCCCGGAACCCAAAG	1550	0.1614310918365859	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1548	0.16122279365357095	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1536	0.15997300455548127	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	1530	0.15934811000643642	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1488	0.15497384816312246	No Hit
GTACATGGGGAATAATTGCAATCCC	1427	0.1486207535811665	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	1421	0.14799585903212165	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1411	0.14695436811704693	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1389	0.14466308810388248	No Hit
ACCTGGAATATGGCGAGAAAACTGA	1344	0.1399763789860461	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1341	0.13966393171152366	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1334	0.13893488807097135	No Hit
GAATAATGGAATAGGACCGCGGTTC	1333	0.13883073897946388	No Hit
GACCTGGAATATGGCGAGAAAACTG	1322	0.13768509897288167	No Hit
GAGTATGGTTGCAAAGCTGAAACTT	1295	0.13487307350217984	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1281	0.1334149862210752	No Hit
GCCATATTCCACGTCCTACAGTGGA	1270	0.132269346214493	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1260	0.13122785529941822	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1237	0.1288324261947463	No Hit
GTCCACTGTAGGACGTGGAATATGG	1220	0.12706189163911924	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1216	0.12664529527308932	No Hit
GTTATTGCTCAATCTCGGGTGGCTG	1212	0.12622869890705943	No Hit
GCCATGCACCACCACCCACGGAATC	1180	0.12289592797882024	No Hit
GATATACACTGTTCTACAATGCCGG	1179	0.12279177888731277	No Hit
GTATCAACGCAGAGTACATGGGGAA	1179	0.12279177888731277	No Hit
CATCTAAGGGCATCACAGACCTGTT	1177	0.12258348070429781	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	1176	0.12247933161279034	No Hit
ATACACACTTTAGGACGTGAAATAT	1172	0.12206273524676044	No Hit
GGATTGACAGATTGATAGCTCTTTC	1137	0.11841751704399883	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	1119	0.11654283339686428	No Hit
CACTTTAGGACGTGAAATATGGCGA	1082	0.11268931701108771	No Hit
CTCTAGATAACCTCGGGCCGATCGC	1071	0.11154367700450549	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1061	0.11050218608943073	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1060	0.11039803699792326	No Hit
TCACAGACCTGTTATTGCTCAATCT	1056	0.10998144063189337	No Hit
GTTCTATTTTGTTGGTTTTCGGAAC	1043	0.1086275024422962	No Hit
CCACTGTAGGACGTGGAATATGGCA	1034	0.10769016061872892	No Hit
GTAGGACGTGGAATATGGCAAGAAA	1033	0.10758601152722144	No Hit
CTTTAATATACGCTATTGGAGCTGG	1031	0.10737771334420651	No Hit
CTCCAATAGCGTATATTAAAGTTGC	1020	0.10623207333762429	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	1017	0.10591962606310186	No Hit
CTCGGGGGTCGCGTAACTAGTTAGC	1011	0.105294731514057	No Hit
GTGTATTTCTCATTTTCCGTGATTT	1009	0.10508643333104206	No Hit
ATCTCGGGTGGCTGAACGCCACTTG	979	0.10196196058581782	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	978	0.10185781149431035	No Hit
TCTAAGGGCATCACAGACCTGTTAT	975	0.1015453642197879	No Hit
GTCCTACAGTGGACATTTCTAAATA	974	0.10144121512828043	No Hit
GAAATACACACTTTAGGACGTGAAA	973	0.10133706603677296	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	967	0.10071217148772812	No Hit
GCTAAGAGCATCGAGGGGGCGCCGA	965	0.10050387330471316	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	1.0414909150747478E-4	0.0	0.0	0.0	0.0
11	1.0414909150747478E-4	0.0	0.0	0.0	0.0
12	1.0414909150747478E-4	0.0	0.0	0.0	0.0
13	2.0829818301494957E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGGTC	65	1.0000804E-8	17.540148	8
TAAGACC	60	7.6812285E-8	17.415615	4
CGAACGA	50	8.716754E-5	15.198289	16
GGTATCA	715	0.0	15.006034	1
GACCTAG	70	4.4260014E-7	14.930006	7
CGTCTTA	45	6.7567313E-4	14.778427	15
GTAAGAC	85	1.7174898E-8	14.527019	3
GTGTAGG	85	1.7258571E-8	14.521715	1
GGACTGT	60	2.563908E-5	14.251369	6
GACCGGG	40	0.005273784	14.251369	7
ACCTGCA	40	0.005273784	14.251369	8
TTGTAGA	335	0.0	14.171593	1
CCAACGA	210	0.0	14.020767	19
CACCTTT	665	0.0	14.001346	14
CGAGACT	75	9.668147E-7	13.932492	4
AGCCCCG	225	0.0	13.931766	16
TGGTCAT	55	1.9538583E-4	13.822395	10
CCACCTT	670	0.0	13.755771	13
CGTCACT	90	3.6308847E-8	13.722825	15
AAACGGG	90	3.643254E-8	13.719246	19
>>END_MODULE