##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062082_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	960162
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.318454594120574	32.0	32.0	32.0	32.0	32.0
2	31.58618441471335	32.0	32.0	32.0	32.0	32.0
3	31.675650567300103	32.0	32.0	32.0	32.0	32.0
4	31.738777414644613	32.0	32.0	32.0	32.0	32.0
5	31.73248889249939	32.0	32.0	32.0	32.0	32.0
6	35.43028155665398	36.0	36.0	36.0	36.0	36.0
7	35.4233462686505	36.0	36.0	36.0	36.0	36.0
8	35.384011239769954	36.0	36.0	36.0	36.0	36.0
9	35.44055065707662	36.0	36.0	36.0	36.0	36.0
10	35.34593120744208	36.0	36.0	36.0	36.0	36.0
11	35.4277538582031	36.0	36.0	36.0	36.0	36.0
12	35.36105782149262	36.0	36.0	36.0	36.0	36.0
13	35.38740233418944	36.0	36.0	36.0	36.0	36.0
14	35.3696647024148	36.0	36.0	36.0	36.0	36.0
15	35.348186035273216	36.0	36.0	36.0	36.0	36.0
16	35.34387113841206	36.0	36.0	36.0	36.0	36.0
17	35.340949756395275	36.0	36.0	36.0	36.0	36.0
18	35.330334880988836	36.0	36.0	36.0	36.0	36.0
19	35.279178930222194	36.0	36.0	36.0	36.0	36.0
20	35.259834277965595	36.0	36.0	36.0	36.0	36.0
21	35.22653677191974	36.0	36.0	36.0	36.0	36.0
22	35.17387170081715	36.0	36.0	36.0	36.0	36.0
23	35.18470424782485	36.0	36.0	36.0	36.0	36.0
24	35.145158837779455	36.0	36.0	36.0	36.0	36.0
25	35.071484811937985	36.0	36.0	36.0	36.0	36.0
26	35.07624859138354	36.0	36.0	36.0	36.0	36.0
27	34.99572155532087	36.0	36.0	36.0	36.0	36.0
28	34.90957775875321	36.0	36.0	36.0	36.0	36.0
29	34.86542791737228	36.0	36.0	36.0	32.0	36.0
30	34.7841541323235	36.0	36.0	36.0	32.0	36.0
31	34.80771786427707	36.0	36.0	36.0	32.0	36.0
32	34.74895069790306	36.0	36.0	36.0	32.0	36.0
33	34.794520091401246	36.0	36.0	36.0	32.0	36.0
34	34.75898858734255	36.0	36.0	36.0	32.0	36.0
35	34.748646582555864	36.0	36.0	36.0	32.0	36.0
36	34.6520358022917	36.0	36.0	36.0	32.0	36.0
37	34.50735188436951	36.0	36.0	36.0	32.0	36.0
38	34.459841151805634	36.0	36.0	36.0	32.0	36.0
39	34.195564915087246	36.0	36.0	36.0	32.0	36.0
40	34.33765447913998	36.0	36.0	36.0	32.0	36.0
41	34.33956873944189	36.0	36.0	36.0	32.0	36.0
42	34.29316511172073	36.0	36.0	36.0	32.0	36.0
43	34.34976076953681	36.0	36.0	36.0	32.0	36.0
44	34.15262528614963	36.0	36.0	36.0	32.0	36.0
45	34.14405069144582	36.0	36.0	36.0	32.0	36.0
46	33.90408701864894	36.0	36.0	36.0	32.0	36.0
47	33.956924977243425	36.0	36.0	36.0	32.0	36.0
48	33.892285885090224	36.0	36.0	36.0	32.0	36.0
49	33.830597336699434	36.0	36.0	36.0	32.0	36.0
50	33.542946919374025	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	1.0
20	14.0
21	20.0
22	71.0
23	166.0
24	425.0
25	1130.0
26	2637.0
27	5384.0
28	9440.0
29	14971.0
30	21859.0
31	30687.0
32	45705.0
33	77276.0
34	168796.0
35	581578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.66970192638279	20.697623537501432	13.222341456299553	25.410333079816215
2	12.29622423770263	20.886677137536402	45.70722115060138	21.10987747415959
3	18.613375493017305	25.930809376363705	30.020548679958214	25.435266450660777
4	10.51010142038531	17.321347855882653	39.471568339509375	32.696982384222665
5	11.497538956967679	40.39547493027219	34.74757384691333	13.359412265846803
6	31.300961611566418	38.907514276281326	17.870894299798262	11.920629812353996
7	27.964135218848483	31.203067815639447	22.021492206523483	18.811304758988587
8	23.812960729543555	41.46841887098219	19.58075824704581	15.137862152428443
9	26.834325874175402	13.75965722451003	20.893453396406024	38.51256350490855
10	17.667643585145008	27.570243354767214	31.318256710846708	23.443856349241067
11	35.884257031625914	19.492960562905008	27.554933438315615	17.06784896715346
12	26.882859350817885	23.04246575057126	32.00209964568479	18.072575252926068
13	30.57431975020882	21.582920382185506	26.03508574594704	21.80767412165864
14	20.21304738158769	25.823142344729327	25.17418935554625	28.789620918136734
15	21.011662615267007	38.511417864901965	20.825027443285613	19.651892076545415
16	20.225650596725327	26.622104509991594	33.02699454360068	20.125250349682396
17	19.43526196620987	31.353563252867744	29.01020869394956	20.200966086972823
18	20.741772977183988	24.994401968840577	36.34398052822501	17.919844525750424
19	26.03896638167974	23.988581081714422	25.993557330489363	23.978895206116473
20	25.79882852369823	26.981865446864884	27.325142997596224	19.89416303184066
21	24.379740085527235	25.332808421912134	24.566583555691643	25.720867936868984
22	25.00796740550032	30.618999710465527	26.03362765866593	18.339405225368218
23	19.53909861044282	29.992959521414093	29.410245354429776	21.05769651371331
24	25.086262113929518	26.24722050085663	29.787065630028486	18.87945175518536
25	21.92382319215215	28.212351886818983	29.5825387617285	20.281286159300368
26	17.559303110340863	31.9503578600055	31.31577083628426	19.174568193369385
27	20.418452834747686	29.05236232445561	29.42678064814468	21.102404192652028
28	18.75160132563725	30.517881729994105	27.548633431304953	23.181883513063692
29	21.2485484114544	26.61861886882847	27.152699303758247	24.980133415958882
30	18.635356215916712	28.160450263917646	30.104733853530913	23.09945966663473
31	24.510755566294574	25.79711171332932	27.56016222397657	22.131970496399536
32	21.22674965395924	29.352484567467275	27.671075366584493	21.749690411988997
33	18.724105824249758	29.71719237875433	31.461384480470922	20.097317316524986
34	21.50861058891533	29.423478500867045	30.798360681348324	18.269550228869296
35	20.8985784675695	29.874989715266505	30.377197157560033	18.84923465960396
36	17.851693622215443	29.615970654921416	31.21538991898234	21.316945803880806
37	20.315679841691402	29.15325730354632	26.918918918918923	23.612143935843356
38	21.06477865193582	30.8387542935463	27.018461467960613	21.078005586557268
39	24.851352435070513	25.609770068853322	27.665163093119144	21.873714402957017
40	26.194356988046795	26.445877305993477	29.233743091002445	18.126022614957282
41	23.562899353743443	25.574782975488077	31.811028542563886	19.051289128204594
42	23.33072253769336	31.071453865991195	28.975561286344565	16.62226230997088
43	19.608886704001847	30.17317428559228	30.643318463834625	19.57462054657125
44	18.446463407140158	30.827845298343227	28.38304418514788	22.342647109368734
45	19.136028129026077	31.516319896849126	28.107571028186307	21.240080945938487
46	19.94098795700224	27.14845601123999	28.88955082732468	24.02100520443309
47	24.12506314084705	26.728392811577294	28.51341710453	20.633126943045653
48	21.194099493709647	28.79751614640238	26.671534108721712	23.336850251166258
49	22.759299473511536	28.383925177188623	27.136183974128635	21.7205913751712
50	20.592747508433675	32.65370951497648	25.39965567485541	21.353887301734435
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	297.0
1	161.5
2	26.0
3	76.0
4	126.0
5	133.5
6	141.0
7	112.0
8	83.0
9	119.5
10	156.0
11	262.5
12	369.0
13	704.5
14	1040.0
15	1392.0
16	1744.0
17	1916.5
18	2089.0
19	2141.0
20	2193.0
21	2657.0
22	3121.0
23	3864.0
24	4607.0
25	6950.0
26	9293.0
27	15595.0
28	21897.0
29	29295.5
30	36694.0
31	45818.5
32	54943.0
33	69053.0
34	83163.0
35	97256.5
36	111350.0
37	108676.5
38	106003.0
39	92325.0
40	78647.0
41	66661.0
42	54675.0
43	44920.0
44	35165.0
45	34803.0
46	34441.0
47	35762.5
48	37084.0
49	38883.0
50	40682.0
51	33148.5
52	25615.0
53	25307.0
54	24999.0
55	29939.5
56	34880.0
57	35499.0
58	36118.0
59	32734.5
60	29351.0
61	25557.0
62	21763.0
63	18657.0
64	15551.0
65	13189.0
66	10827.0
67	10549.0
68	10271.0
69	10635.5
70	11000.0
71	8495.0
72	5990.0
73	5768.0
74	5546.0
75	3684.0
76	1822.0
77	1948.5
78	2075.0
79	1832.5
80	1590.0
81	1299.5
82	1009.0
83	933.5
84	858.0
85	697.5
86	537.0
87	374.5
88	212.0
89	135.0
90	58.0
91	39.5
92	21.0
93	17.0
94	13.0
95	9.5
96	6.0
97	10.5
98	15.0
99	10.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03457749838048163
2	0.003957665477284041
3	3.1244727452242436E-4
4	0.0
5	0.0
6	3.1244727452242436E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.207454575373738E-4
17	0.0
18	3.1244727452242436E-4
19	1.0414909150747478E-4
20	6.248945490448487E-4
21	0.0
22	0.0
23	0.0
24	7.290436405523235E-4
25	1.0414909150747478E-4
26	0.0010414909150747477
27	6.248945490448487E-4
28	0.0020829818301494954
29	7.290436405523235E-4
30	0.001458087281104647
31	8.331927320597983E-4
32	0.0015622363726121218
33	4.1659636602989913E-4
34	7.290436405523235E-4
35	1.0414909150747478E-4
36	1.0414909150747478E-4
37	0.0012497890980896975
38	0.0
39	5.207454575373738E-4
40	1.0414909150747478E-4
41	0.001770534555627071
42	0.00479085820934384
43	0.0032286218367317183
44	0.004478410934821415
45	0.0015622363726121218
46	0.0019788327386420206
47	7.290436405523235E-4
48	0.004270112751806466
49	0.002812025470701819
50	0.00239542910467192
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	960162.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.58175825157465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.69944980177446	30.35899080082622
2	13.217615306319269	10.463529074340844
3	4.219702356865416	5.010697157491402
4	1.791377226255752	2.8362344122812604
5	0.9410479091609384	1.8624165421779022
6	0.5917757427705975	1.405411463369509
7	0.3746183554592229	1.0379637227672596
8	0.29024000753891355	0.9190567850672383
9	0.20233534872329037	0.7207909973021999
>10	1.2659870040516743	9.837454260740625
>50	0.17808844734417395	5.002629508829144
>100	0.18680752096506872	15.627217760228874
>500	0.030121721909163957	8.173434090073666
>1k	0.01083325086206774	6.744173424503851
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	3491	0.36358447845259445	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	2804	0.29203405258695925	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	2554	0.26599677971009056	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	2526	0.2630806051478813	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2273	0.23673088499649017	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2157	0.22464959038162308	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2078	0.21642181215253262	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2073	0.2159010666949952	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2028	0.21121435757715887	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1984	0.20663179755082997	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1685	0.175491219190095	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1655	0.17236674644487077	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1638	0.17059621188924368	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	1617	0.16840908096758672	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1608	0.16747173914401944	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1578	0.1643472663987952	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1481	0.15424480452257014	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	1467	0.1527867172414655	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1415	0.14737096448307682	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	1402	0.14601702629347962	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1385	0.14424649173785256	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC	1366	0.14226765899921054	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	1312	0.13664360805780693	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC	1311	0.13653945896629943	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT	1310	0.13643530987479197	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	1297	0.1350813716851948	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1286	0.13393573167861259	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT	1273	0.1325817934890154	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	1243	0.12945732074379115	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	1242	0.1293531716522837	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	1227	0.12779093527967156	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1170	0.12185443706374549	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	1153	0.12008390250811843	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG	1083	0.11279346610259518	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC	1072	0.11164782609601295	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC	1069	0.11133537882149053	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	1061	0.11050218608943073	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC	1049	0.10925239699134104	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA	1028	0.10706526606968408	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC	1021	0.10633622242913175	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	1013	0.10550302969707195	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	996	0.10373249514144488	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	979	0.10196196058581782	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	977	0.10175366240280286	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	973	0.10133706603677296	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	1.0414909150747478E-4	0.0
21	1.0414909150747478E-4	0.0	0.0	2.0829818301494957E-4	0.0
22	1.0414909150747478E-4	0.0	0.0	4.1659636602989913E-4	0.0
23	1.0414909150747478E-4	0.0	0.0	6.248945490448487E-4	0.0
24	1.0414909150747478E-4	0.0	0.0	7.290436405523235E-4	0.0
25	1.0414909150747478E-4	0.0	0.0	0.0011456400065822227	0.0
26	1.0414909150747478E-4	0.0	0.0	0.0016663854641195965	0.0
27	1.0414909150747478E-4	0.0	0.0	0.0021871309216569706	0.0
28	1.0414909150747478E-4	0.0	0.0	0.003436920019746668	0.0
29	1.0414909150747478E-4	0.0	0.0	0.003957665477284041	0.0
30	1.0414909150747478E-4	0.0	0.0	0.005832349124418588	0.0
31	2.0829818301494957E-4	0.0	0.0	0.010935654608284853	0.0
32	2.0829818301494957E-4	0.0	0.0	0.01614310918365859	0.0
33	2.0829818301494957E-4	0.0	0.0	0.023329396497674352	0.0
34	2.0829818301494957E-4	0.0	0.0	0.027703658340988292	0.0
35	2.0829818301494957E-4	0.0	0.0	0.03364015655691435	0.0
36	2.0829818301494957E-4	0.0	0.0	0.04072229477942264	0.0
37	2.0829818301494957E-4	0.0	0.0	0.052595291211274764	0.0
38	2.0829818301494957E-4	0.0	0.0	0.06759276038835113	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAACA	35	3.2166147E-4	31.429834	39
ACGGAAC	35	3.2166147E-4	31.429834	38
TGGCGTG	35	3.21858E-4	31.426561	18
CGAGAAC	30	0.0057447646	29.332987	21
GGTATCA	775	0.0	29.245977	1
CTGTGCA	130	0.0	27.075193	9
GTATCAA	1875	0.0	26.876013	1
ACTGTGC	115	0.0	26.780897	8
GTGTAGG	155	0.0	25.554739	1
CTCGTCT	80	1.3100544E-7	24.748417	13
CACGTTA	45	0.0013972528	24.446701	28
TAGAACG	45	0.0013985252	24.44288	4
CTTCTCG	90	1.5128535E-8	24.44288	10
GACGTGT	55	1.593976E-4	23.998466	7
ACTGTTC	895	0.0	23.84205	8
AGTGAAC	65	1.813784E-5	23.690794	8
AGGACCT	2475	0.0	23.55405	5
TAAGACG	75	2.0646785E-6	23.465166	4
CTATAGG	200	0.0	23.105742	1
GGACCTG	2490	0.0	22.88207	6
>>END_MODULE