FastQCFastQC Report
Thu 2 Feb 2017
SRR4062081_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062081_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978070
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30410.15373571208299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7250.013.622541
CGCTCGA550.0030658312.09255210
GTATAAA1750.011.9384351
GGTCTAT1052.734214E-711.757551
GTATCAA17250.011.4508311
GCTATAT700.001496327610.8531231
CCCTAAT803.7845783E-410.6835431
TAGTACA1002.3996992E-510.4498334
TGGACCG1752.3646862E-1010.3143825
CTTACAC2250.010.1318893
CTAGACT1606.175469E-910.0935894
ATTATAC1606.188202E-910.0923113
ACCGGGC856.5894634E-410.0596818
GTTCTAT1701.5697879E-910.05509951
AAGACCG1054.1053187E-59.9524745
GTGTAGC3950.09.8571081
TCTTAAG1451.6224476E-79.8254392
GTGTTAG1554.0870873E-89.8028211
GTATAAT1856.530172E-109.7531451
TATACAG2850.09.6667575
Produced by FastQC (version 0.11.5)