##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062081_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1978070
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25504051929406	32.0	32.0	32.0	32.0	32.0
2	30.791411325180604	32.0	32.0	32.0	32.0	32.0
3	30.82128337217591	32.0	32.0	32.0	32.0	32.0
4	30.81638617440232	32.0	32.0	32.0	32.0	32.0
5	30.738184695182678	32.0	32.0	32.0	32.0	32.0
6	34.39276365346019	36.0	36.0	36.0	32.0	36.0
7	34.33773577274818	36.0	36.0	36.0	32.0	36.0
8	34.292403201100065	36.0	36.0	36.0	32.0	36.0
9	34.42528323062379	36.0	36.0	36.0	32.0	36.0
10	34.11628051585637	36.0	36.0	36.0	32.0	36.0
11	34.40643859924067	36.0	36.0	36.0	32.0	36.0
12	34.23411962165141	36.0	36.0	36.0	32.0	36.0
13	34.304383565798986	36.0	36.0	36.0	32.0	36.0
14	34.22529182485958	36.0	36.0	36.0	32.0	36.0
15	34.17768582507191	36.0	36.0	36.0	32.0	36.0
16	34.17781676078197	36.0	36.0	36.0	32.0	36.0
17	34.125662893628636	36.0	36.0	36.0	32.0	36.0
18	34.11429676401745	36.0	36.0	36.0	32.0	36.0
19	34.13148220234876	36.0	36.0	36.0	32.0	36.0
20	34.11149200988843	36.0	36.0	36.0	32.0	36.0
21	34.10050908208506	36.0	36.0	36.0	32.0	36.0
22	34.06560030737032	36.0	36.0	36.0	32.0	36.0
23	34.02806978519466	36.0	36.0	36.0	32.0	36.0
24	34.009674076246036	36.0	36.0	36.0	32.0	36.0
25	33.65303452355074	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	31.0
5	166.0
6	437.0
7	77.0
8	377.0
9	272.0
10	138.0
11	46.0
12	91.0
13	80.0
14	282.0
15	414.0
16	698.0
17	947.0
18	1363.0
19	1844.0
20	2870.0
21	3960.0
22	5845.0
23	8816.0
24	12489.0
25	17317.0
26	23367.0
27	30085.0
28	39642.0
29	52339.0
30	67287.0
31	90531.0
32	127058.0
33	177761.0
34	392922.0
35	918518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.74465611151162	16.580993238809455	11.424410051495055	26.249940598183873
2	17.087085523392282	18.456705460431824	37.447518208432776	27.008690807743115
3	20.08896613644912	21.25864990677716	27.810891097117572	30.84149285965615
4	13.784839528094592	14.93219418622406	33.62331092183751	37.65965536384384
5	15.648097341664286	35.356497156569475	33.2153648921671	15.780040609599139
6	36.236277203400164	33.36390572506398	15.908250448088737	14.491566623447119
7	31.82796123403667	29.399141739508476	19.10396349533392	19.668933531120928
8	28.39066282484926	33.73138286699926	18.126370013984204	19.751584294167277
9	26.824752307862067	15.255730047711168	18.09248455435662	39.827033090070145
10	16.92734027866722	26.731773054743734	30.780410979223227	25.560475687365813
11	36.97058187707541	21.39082862539749	21.331442564870283	20.307146932656813
12	24.53481502705532	23.009906363125996	28.332330159456554	24.12294845036213
13	30.22956329821968	19.307564199261957	24.311841619041353	26.151030883477013
14	23.448664369122753	18.858570464733848	24.788470911808428	32.90429425433498
15	25.416907548833688	26.41600746793778	21.676863871117323	26.4902211121112
16	25.78075016804477	25.985693923663135	22.958256059734854	25.275299848557243
17	24.659795421689672	25.87260626774166	24.680143917520763	24.7874543930479
18	25.033694853936666	25.551555530587034	24.974023456578305	24.440726158897995
19	25.94996890325395	25.031741959251875	24.631912194327082	24.38637694316709
20	25.749069308614075	24.88534924520005	24.763955816637733	24.601625629548142
21	25.811012401754503	24.442356232149834	24.66004983371893	25.086581532376734
22	25.90198463948955	24.40267493127447	24.618821281230137	25.076519148005843
23	25.293589651477085	24.5599823517328	24.747292802360658	25.399135194429462
24	25.108149752428275	24.55916546697062	24.890104468435553	25.442580312165553
25	25.335644077873777	24.499323221717624	24.699649593300528	25.46538310710807
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	122.0
1	122.0
2	316.5
3	511.0
4	511.0
5	511.0
6	1060.0
7	1609.0
8	1609.0
9	1609.0
10	2048.0
11	2487.0
12	2487.0
13	2487.0
14	3271.5
15	4056.0
16	4056.0
17	4056.0
18	6932.5
19	9809.0
20	9809.0
21	9809.0
22	15551.5
23	21294.0
24	21294.0
25	21294.0
26	31451.5
27	41609.0
28	41609.0
29	41609.0
30	55289.0
31	68969.0
32	68969.0
33	68969.0
34	87561.0
35	106153.0
36	106153.0
37	106153.0
38	130129.5
39	154106.0
40	154106.0
41	154106.0
42	180628.0
43	207150.0
44	207150.0
45	207150.0
46	229958.0
47	252766.0
48	252766.0
49	252766.0
50	264749.5
51	276733.0
52	276733.0
53	276733.0
54	269103.5
55	261474.0
56	261474.0
57	261474.0
58	238632.0
59	215790.0
60	215790.0
61	215790.0
62	186373.5
63	156957.0
64	156957.0
65	156957.0
66	126988.5
67	97020.0
68	97020.0
69	97020.0
70	74709.0
71	52398.0
72	52398.0
73	52398.0
74	38974.0
75	25550.0
76	25550.0
77	25550.0
78	19044.5
79	12539.0
80	12539.0
81	12539.0
82	8881.0
83	5223.0
84	5223.0
85	5223.0
86	3627.5
87	2032.0
88	2032.0
89	2032.0
90	1373.5
91	715.0
92	715.0
93	715.0
94	474.0
95	233.0
96	233.0
97	233.0
98	499.0
99	765.0
100	765.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.088692513409535E-4
2	1.5166298462642878E-4
3	7.077605949233343E-4
4	0.010919734893102873
5	0.03564080138721077
6	0.06779335412801367
7	0.11187672832609565
8	0.11470777070578897
9	0.12294812620382493
10	0.13846830496392948
11	0.14453482434898665
12	0.13513171930214807
13	0.13043016677872876
14	0.11814546502398804
15	0.13609225153811544
16	0.1216337136703959
17	0.12608249455277112
18	0.11369668414161278
19	0.10004701552523418
20	0.09357606151450656
21	0.09595211494032062
22	0.10586076326924729
23	0.08452683676512965
24	0.094789365391518
25	0.09074501913481323
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1978070.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.20180315023376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.58987235321099	35.28313211531748
2	16.290667797234295	17.00804467074154
3	6.732985284550877	10.544219173126368
4	3.290752889698809	6.871329382564806
5	1.837138877373629	4.795098101814981
6	1.1341600389943582	3.5523119457866965
7	0.7185179241312921	2.6255551864791418
8	0.5216598289841801	2.1785266963213425
9	0.35068080718084654	1.6475553418517672
>10	1.4761133201328724	12.350925635482035
>50	0.043723720894867216	1.5151493482105096
>100	0.012656930487382981	1.0488542530472595
>500	7.783470005218645E-4	0.2505924135042927
>1k	2.9188012519569686E-4	0.32870573575187906
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3041	0.15373571208299	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0110865641761919E-4
2	0.0	0.0	0.0	0.0	1.0110865641761919E-4
3	0.0	0.0	0.0	0.0	1.0110865641761919E-4
4	0.0	0.0	0.0	0.0	1.0110865641761919E-4
5	0.0	0.0	0.0	0.0	1.0110865641761919E-4
6	0.0	0.0	0.0	0.0	1.0110865641761919E-4
7	0.0	0.0	0.0	0.0	1.0110865641761919E-4
8	0.0	0.0	0.0	0.0	1.5166298462642878E-4
9	0.0	0.0	0.0	0.0	1.5166298462642878E-4
10	0.0	0.0	0.0	0.0	1.5166298462642878E-4
11	0.0	0.0	0.0	0.0	1.5166298462642878E-4
12	0.0	0.0	0.0	0.0	1.5166298462642878E-4
13	0.0	0.0	0.0	0.0	1.5166298462642878E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	725	0.0	13.62254	1
CGCTCGA	55	0.00306583	12.092552	10
GTATAAA	175	0.0	11.938435	1
GGTCTAT	105	2.734214E-7	11.75755	1
GTATCAA	1725	0.0	11.450831	1
GCTATAT	70	0.0014963276	10.853123	1
CCCTAAT	80	3.7845783E-4	10.683543	1
TAGTACA	100	2.3996992E-5	10.449833	4
TGGACCG	175	2.3646862E-10	10.314382	5
CTTACAC	225	0.0	10.131889	3
CTAGACT	160	6.175469E-9	10.093589	4
ATTATAC	160	6.188202E-9	10.092311	3
ACCGGGC	85	6.5894634E-4	10.059681	8
GTTCTAT	170	1.5697879E-9	10.0550995	1
AAGACCG	105	4.1053187E-5	9.952474	5
GTGTAGC	395	0.0	9.857108	1
TCTTAAG	145	1.6224476E-7	9.825439	2
GTGTTAG	155	4.0870873E-8	9.802821	1
GTATAAT	185	6.530172E-10	9.753145	1
TATACAG	285	0.0	9.666757	5
>>END_MODULE