FastQCFastQC Report
Thu 2 Feb 2017
SRR4062081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978070
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18850.025.33911
GGTATCA9150.025.2587011
TATCAAC25850.018.2974322
CAACGCA25650.018.0965735
TCAACGC25850.017.956564
ATCAACG26500.017.765163
ACCGTTA750.00129169217.599138
TACACCG1903.274181E-1117.3675635
AACGCAG26900.017.3374336
GGACCGT1651.6625563E-917.3324786
GTCTTAG2950.015.6689571
TACGCCG850.002981818315.52864555
TTAGACT2152.1645974E-1015.3480794
CTTAGAC2355.0931703E-1114.9779833
TAGCGTG1201.182118E-414.6659425
ACGCAGA32200.014.5520757
GTGTATA3050.014.4335421
GACCGTT2153.5506673E-914.3248737
TAGGACG2153.5506673E-914.3248734
TATACAG4150.014.3125475
Produced by FastQC (version 0.11.5)