##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062081_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1978070
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.289142952473878	32.0	32.0	32.0	32.0	32.0
2	31.46483643147108	32.0	32.0	32.0	32.0	32.0
3	31.580582082534995	32.0	32.0	32.0	32.0	32.0
4	31.658897308993108	32.0	32.0	32.0	32.0	32.0
5	31.637688251679666	32.0	32.0	32.0	32.0	32.0
6	35.28806311202334	36.0	36.0	36.0	36.0	36.0
7	35.29644603072692	36.0	36.0	36.0	36.0	36.0
8	35.248372403403316	36.0	36.0	36.0	36.0	36.0
9	35.334504340089076	36.0	36.0	36.0	36.0	36.0
10	35.21633410344427	36.0	36.0	36.0	36.0	36.0
11	35.32736910220569	36.0	36.0	36.0	36.0	36.0
12	35.26333092357702	36.0	36.0	36.0	36.0	36.0
13	35.28649238904589	36.0	36.0	36.0	36.0	36.0
14	35.25174538818141	36.0	36.0	36.0	36.0	36.0
15	35.23687230482238	36.0	36.0	36.0	36.0	36.0
16	35.25218976072637	36.0	36.0	36.0	36.0	36.0
17	35.23152112918147	36.0	36.0	36.0	36.0	36.0
18	35.22287077808166	36.0	36.0	36.0	36.0	36.0
19	35.222880383404025	36.0	36.0	36.0	36.0	36.0
20	35.21762475544344	36.0	36.0	36.0	36.0	36.0
21	35.21379779279803	36.0	36.0	36.0	36.0	36.0
22	35.194071493930956	36.0	36.0	36.0	36.0	36.0
23	35.16453816093465	36.0	36.0	36.0	36.0	36.0
24	35.154860040342356	36.0	36.0	36.0	36.0	36.0
25	35.14362080209497	36.0	36.0	36.0	36.0	36.0
26	35.099321560915435	36.0	36.0	36.0	36.0	36.0
27	35.0872031829005	36.0	36.0	36.0	36.0	36.0
28	35.07444225937404	36.0	36.0	36.0	36.0	36.0
29	35.05812534440136	36.0	36.0	36.0	36.0	36.0
30	35.04029382175555	36.0	36.0	36.0	36.0	36.0
31	35.03412568817079	36.0	36.0	36.0	36.0	36.0
32	35.00911191211635	36.0	36.0	36.0	36.0	36.0
33	34.992531103550434	36.0	36.0	36.0	36.0	36.0
34	34.982714969642124	36.0	36.0	36.0	36.0	36.0
35	34.97642702229951	36.0	36.0	36.0	36.0	36.0
36	34.948413857952445	36.0	36.0	36.0	36.0	36.0
37	34.940828686548	36.0	36.0	36.0	36.0	36.0
38	34.93406552852022	36.0	36.0	36.0	36.0	36.0
39	34.91092226260951	36.0	36.0	36.0	36.0	36.0
40	34.90724089642935	36.0	36.0	36.0	36.0	36.0
41	34.88330443310904	36.0	36.0	36.0	36.0	36.0
42	34.86000647095401	36.0	36.0	36.0	32.0	36.0
43	34.84979854100209	36.0	36.0	36.0	32.0	36.0
44	34.82428832144464	36.0	36.0	36.0	32.0	36.0
45	34.806465898577905	36.0	36.0	36.0	32.0	36.0
46	34.78866268635589	36.0	36.0	36.0	32.0	36.0
47	34.759450878886994	36.0	36.0	36.0	32.0	36.0
48	34.74132411896444	36.0	36.0	36.0	32.0	36.0
49	34.72862133291542	36.0	36.0	36.0	32.0	36.0
50	34.32230456960573	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	3.0
20	14.0
21	52.0
22	102.0
23	291.0
24	849.0
25	2053.0
26	4433.0
27	8209.0
28	14493.0
29	23188.0
30	34401.0
31	51019.0
32	78558.0
33	132230.0
34	302976.0
35	1325195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.301700387168815	17.118919892062028	11.848599597862277	26.730780122906882
2	16.45371490567955	18.964697008227034	37.102595512315276	27.478992573778143
3	19.413325352465847	21.714262025900073	28.03756602415291	30.834846597481175
4	13.517873482738224	15.303300692088753	33.78363758613194	37.39518823904108
5	15.297689161657576	35.65814152178638	33.06455282168983	15.979616494866208
6	36.35580041899554	33.49800613124752	15.794534453890268	14.351658995866664
7	31.903623228702727	29.372216352303006	19.083146703605028	19.641013715389242
8	28.36441581946038	33.920639815577815	18.0397053693752	19.675238995586607
9	26.88782500113747	14.997447006425455	18.03121224223612	40.08351575020095
10	16.799725793046548	26.818491578651493	30.883973655101848	25.497808973200115
11	37.310914173917	21.23660942231569	21.2452542124394	20.20722219132791
12	24.57745407263346	22.961736926264958	28.330103752700236	24.130705248401345
13	30.33588295661933	19.207358688014075	24.43998442926691	26.016773926099685
14	23.546790558473663	18.62355730585874	24.801144549990646	33.02850758567695
15	25.50051312643132	26.508819202556026	21.66945558043952	26.32121209057313
16	25.946599058165727	25.852415630505725	22.973375361150218	25.22760995017833
17	24.777839004686385	25.780482996051706	24.65059376058481	24.791084238677094
18	25.105633482401498	25.468108748646408	24.970905925282576	24.455351843669522
19	26.016736524357846	24.936996636618844	24.757326463333683	24.288940375689624
20	25.89921074503	24.63484571814518	24.83246278692138	24.63348074990344
21	25.955855960608066	24.3050549272776	24.6694505250067	25.069638587107633
22	26.021273261310267	24.145758239091638	24.696497090598413	25.136471408999682
23	25.418716223389463	24.3980243368536	24.85083945461991	25.33241998513703
24	25.26097658756018	24.414587894852055	24.87509169379354	25.44934382379423
25	25.498492969381143	24.218047765944647	24.84184014454552	25.44161912012869
26	25.269038720241692	24.687155755055077	24.893216134386147	25.150589390317084
27	25.422320994794383	24.77734463668135	24.68796399699301	25.112370371531256
28	25.49046174130475	24.541135288877744	24.69492435418198	25.273478615635526
29	25.354286523159054	24.788479621447276	24.84211803484222	25.015115820551447
30	25.519958100022045	24.683930185577267	24.637621812254935	25.15848990214576
31	25.50940191248474	24.446337437533337	24.643601922090134	25.400658727891795
32	25.381928593419893	24.71490905450966	24.664000408482483	25.239161943587963
33	25.287275550967458	24.64230212743154	24.754280190853088	25.316142130747917
34	25.56888010584145	24.380539854088845	24.899535047847554	25.15104499222215
35	25.45894552611211	24.604323311596623	24.83242478642028	25.104306375870987
36	25.33760282781548	24.671850860235057	24.861581829506022	25.12896448244344
37	25.660047319568868	24.492578512062448	24.654000930213744	25.193373238154944
38	25.427209350528546	24.676780902596978	24.804683352965267	25.091326393909213
39	25.4913925417532	24.638381599674833	24.705113920159835	25.16511193841213
40	25.676619812867905	24.46816792951506	24.570489993266158	25.284722264350872
41	25.273084741581226	24.767991086166216	24.775220431849768	25.18370374040279
42	25.3490828218238	24.68107437477534	24.798669746593603	25.171173056807262
43	25.49911880063863	24.36867601691822	24.7046743505768	25.427530831866353
44	25.276479744872702	24.740431336207962	24.862272010062725	25.120816908856604
45	25.4182199829527	24.578140024448615	24.685570361562487	25.318069631036195
46	25.36575624676129	24.49357313481882	24.784166024190796	25.35650459422909
47	25.212924452326508	24.85989983059065	24.706162486369145	25.221013230713694
48	25.33889046455534	24.932520188331743	24.531559419393034	25.197029927719882
49	25.510213367468893	24.66637950733975	24.54484235559627	25.278564769595086
50	25.179773917615417	24.979373521263472	24.63681219793331	25.2040403631878
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	27.0
2	45.0
3	105.0
4	165.0
5	222.5
6	280.0
7	228.5
8	177.0
9	235.0
10	293.0
11	473.5
12	654.0
13	1260.5
14	1867.0
15	2365.5
16	2864.0
17	2895.0
18	2926.0
19	2855.5
20	2785.0
21	3226.5
22	3668.0
23	4576.0
24	5484.0
25	7058.0
26	8632.0
27	11055.0
28	13478.0
29	15835.0
30	18192.0
31	22151.5
32	26111.0
33	31425.5
34	36740.0
35	42993.5
36	49247.0
37	56302.5
38	63358.0
39	71102.0
40	78846.0
41	87077.0
42	95308.0
43	103880.5
44	112453.0
45	121044.5
46	129636.0
47	136614.5
48	143593.0
49	148787.0
50	153981.0
51	157043.0
52	160105.0
53	159318.5
54	158532.0
55	155791.0
56	153050.0
57	145697.5
58	138345.0
59	127682.5
60	117020.0
61	103852.0
62	90684.0
63	78713.0
64	66742.0
65	56357.0
66	45972.0
67	38901.0
68	31830.0
69	27121.5
70	22413.0
71	18523.0
72	14633.0
73	12334.0
74	10035.0
75	8513.0
76	6991.0
77	5824.0
78	4657.0
79	3811.5
80	2966.0
81	2316.0
82	1666.0
83	1249.0
84	832.0
85	641.5
86	451.0
87	315.0
88	179.0
89	121.0
90	63.0
91	46.0
92	29.0
93	27.5
94	26.0
95	28.5
96	31.0
97	26.0
98	21.0
99	13.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03225366139722052
2	0.003993791928495958
3	3.0332596925285757E-4
4	0.0
5	0.0
6	3.0332596925285757E-4
7	0.0
8	0.0
9	0.0
10	1.0110865641761919E-4
11	0.0
12	5.0554328208809595E-5
13	0.0
14	0.0
15	0.0
16	7.583149231321439E-4
17	0.0
18	2.0221731283523838E-4
19	5.0554328208809595E-5
20	1.0110865641761919E-4
21	0.0
22	0.0
23	0.0
24	9.605322359673823E-4
25	4.0443462567047676E-4
26	4.5498895387928635E-4
27	6.572062667145248E-4
28	0.0018199558155171454
29	5.055432820880959E-4
30	0.0013144125334290497
31	7.583149231321439E-4
32	8.594235795497632E-4
33	2.0221731283523838E-4
34	8.594235795497632E-4
35	1.5166298462642878E-4
36	3.0332596925285757E-4
37	0.0015166298462642878
38	0.0
39	9.099779077585727E-4
40	1.0110865641761919E-4
41	0.0010616408923850016
42	0.005105987149089769
43	0.004246563569540006
44	0.0038926832720783387
45	0.002224390441187622
46	0.002274944769396432
47	0.0010616408923850016
48	0.0035893573028254813
49	0.00374102028745191
50	0.0015166298462642878
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1978070.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.65850668759536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.84189640694304	34.01294045862028
2	17.162347036544404	17.731624383243233
3	7.106018372201264	11.012588928076038
4	3.5146517943934983	7.2624665290098225
5	1.9515485951660558	5.040704307727652
6	1.1941769944684528	3.701364015295266
7	0.778316675915998	2.8144674045511504
8	0.5169420440882546	2.1363563233305856
9	0.3860026953370754	1.7946290536650127
>10	1.499918172691074	12.407550162234775
>50	0.03872467654040429	1.3181697106478154
>100	0.009161268754870075	0.6568083285633418
>500	2.952669556844045E-4	0.11033039503507248
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	5.0554328208809595E-5
5	0.0	0.0	0.0	0.0	5.0554328208809595E-5
6	0.0	0.0	0.0	0.0	5.0554328208809595E-5
7	0.0	0.0	0.0	0.0	5.0554328208809595E-5
8	0.0	0.0	0.0	0.0	1.0110865641761919E-4
9	0.0	0.0	0.0	0.0	1.0110865641761919E-4
10	0.0	0.0	0.0	0.0	1.0110865641761919E-4
11	0.0	0.0	0.0	0.0	1.0110865641761919E-4
12	0.0	0.0	0.0	0.0	1.0110865641761919E-4
13	0.0	0.0	0.0	0.0	1.0110865641761919E-4
14	0.0	0.0	0.0	0.0	3.0332596925285757E-4
15	0.0	0.0	0.0	5.0554328208809595E-5	3.0332596925285757E-4
16	0.0	0.0	0.0	1.0110865641761919E-4	3.5388029746166716E-4
17	0.0	0.0	0.0	1.5166298462642878E-4	3.5388029746166716E-4
18	0.0	0.0	0.0	2.5277164104404797E-4	4.0443462567047676E-4
19	0.0	0.0	0.0	2.5277164104404797E-4	4.0443462567047676E-4
20	0.0	0.0	0.0	3.5388029746166716E-4	5.055432820880959E-4
21	0.0	0.0	0.0	3.5388029746166716E-4	5.560976102969055E-4
22	0.0	0.0	0.0	6.066519385057151E-4	5.560976102969055E-4
23	0.0	0.0	0.0	7.583149231321439E-4	7.077605949233343E-4
24	0.0	0.0	0.0	9.099779077585727E-4	7.077605949233343E-4
25	0.0	0.0	0.0	0.0011627495488026208	7.077605949233343E-4
26	0.0	0.0	0.0	0.0014660755180554784	7.077605949233343E-4
27	0.0	0.0	0.0	0.002224390441187622	7.077605949233343E-4
28	0.0	0.0	0.0	0.003235477005363814	7.077605949233343E-4
29	0.0	0.0	0.0	0.004600443867001674	7.077605949233343E-4
30	0.0	0.0	0.0	0.00748204057490382	7.077605949233343E-4
31	0.0	0.0	0.0	0.01364966861637859	7.077605949233343E-4
32	0.0	0.0	0.0	0.020676720237403124	7.583149231321439E-4
33	0.0	0.0	0.0	0.0279059891712629	7.583149231321439E-4
34	0.0	0.0	0.0	0.03513525810512267	7.583149231321439E-4
35	0.0	0.0	0.0	0.04509446076225816	7.583149231321439E-4
36	0.0	0.0	0.0	0.05768248848625175	7.583149231321439E-4
37	0.0	0.0	0.0	0.07739867648768749	7.583149231321439E-4
38	0.0	0.0	0.0	0.10297916656134515	8.088692513409535E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1885	0.0	25.3391	1
GGTATCA	915	0.0	25.258701	1
TATCAAC	2585	0.0	18.297432	2
CAACGCA	2565	0.0	18.096573	5
TCAACGC	2585	0.0	17.95656	4
ATCAACG	2650	0.0	17.76516	3
ACCGTTA	75	0.001291692	17.59913	8
TACACCG	190	3.274181E-11	17.367563	5
AACGCAG	2690	0.0	17.337433	6
GGACCGT	165	1.6625563E-9	17.332478	6
GTCTTAG	295	0.0	15.668957	1
TACGCCG	85	0.0029818183	15.5286455	5
TTAGACT	215	2.1645974E-10	15.348079	4
CTTAGAC	235	5.0931703E-11	14.977983	3
TAGCGTG	120	1.182118E-4	14.665942	5
ACGCAGA	3220	0.0	14.552075	7
GTGTATA	305	0.0	14.433542	1
GACCGTT	215	3.5506673E-9	14.324873	7
TAGGACG	215	3.5506673E-9	14.324873	4
TATACAG	415	0.0	14.312547	5
>>END_MODULE