FastQCFastQC Report
Thu 2 Feb 2017
SRR4062080_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062080_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767735
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30570.1729331602304644No Hit
GCGCAAGACGGACCAGAGCGAAAGC27060.15307724291254063No Hit
GAATAGGACCGCGGTTCTATTTTGT23770.1344658560248001No Hit
TCGTAGTTCCGACCATAAACGATGC23200.1312413908193253No Hit
GGGTAGGCACACGCTGAGCCAGTCA22370.12654611692363393No Hit
ATCAGATACCGTCGTAGTTCCGACC21390.12100229955281758No Hit
GATTAAGAGGGACGGCCGGGGGCAT21270.12032346477271762No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG21230.1200971865126843No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC20170.11410081262180134No Hit
GAACTACGACGGTATCTGATCGTCT19670.11127233437138485No Hit
GTCTTGCGCCGGTCCAAGAATTTCA19470.11014094307121826No Hit
GAATAACGCCGCCGCATCGCCAGTC19080.10793473003589339No Hit
GTATCTGATCGTCTTCGAACCTCCG18400.10408799961532697No Hit
ACCATACTCCCCCCGGAACCCAAAG18240.10318288657519367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG804.311005E-1016.6263395
CGTTACA456.7777414E-414.7735272
CGAACGA1250.014.43993616
GGTATCA7500.014.181381
CGCATCG3150.013.57213713
GCGTTAT2250.013.5060771
GCATCGC3300.013.24311514
ATAACGA1403.6379788E-1212.89389612
CGCGTAA1850.012.83850910
CGCCAGT3600.012.66661118
GTATCAA21700.012.5160711
TCGCGTA1900.012.5013619
ATCGCCA3500.012.48565916
TCGCCAG3700.012.32426917
CGTTATT2400.012.26730252
GCGTAAC1800.012.13952311
CCGCGCG550.003066227812.0922269
ACGCCCC2600.012.05763817
TACTATA1501.4551915E-1112.0298722
ACCGTCG3800.011.751288
Produced by FastQC (version 0.11.5)